ISSN:
1573-5044
Keywords:
Nicotiana tabacum
;
oxidative burst
;
oxygen electrode
;
Pseudomonas solanacearum
;
Pseudomonas syringae
;
respiratory burst
;
Solanumtuberosum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A method is described that allows the rate of oxygen consumption to be monitored in plant cell suspensions. The method utilized oxygen electrodes placed in beakers of plant cells subjected to various treatments. The voltage readings from calibrated electrodes were converted to % oxygen (100% equals air equilibration) and the rate of oxygen consumption was estimated by calibration graphs made with no cells present. This system simultaneously monitors one to sixteen or more samples, allowing comparison of treatments on identically treated cells. We have used this method to study the respiratory burst of plant cells produced in response to viable or heat-killed bacteria. Because the system was computer-monitored and open to the atmosphere, data could be collected over several hours. Various factors that affected the measurement of dissolved oxygen concentration with this technique were explored and considered.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005997126293
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