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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 23 (2000), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Plant acclimation to freezing temperatures is very complex. Many temperate plants increase in freezing tolerance upon exposure to a period of low but non-freezing temperatures, an adaptive process known as cold acclimation. This acclimation phenomenon has encouraged investigations of physiological, biochemical, and molecular changes that are associated with the development of freezing tolerance. Although many biochemical and gene-expression changes occur during cold acclimation, few have been unequivocally demonstrated to contribute to the development of freezing tolerance. However, in the last few years, exciting new progress has been made through the use of mutational analysis and molecular genetic approaches. We now recognize that several interacting signal pathways are activated to bring about cold acclimation and ensure the winter survival of plants. The challenge for the future is to understand these pathways at a mechanistic level. Facile map-based cloning in Arabidopsis and techniques (such as DNA micro-arrays) for transcript profiling will provide the tools needed for this task.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Planta 166 (1985), S. 74-80 
    ISSN: 1432-2048
    Keywords: Carthamus ; Fatty acid synthesis ; Linum ; Oilseed ; Plastid (fatty acid synthesis)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plastids isolated from maturing, nongreen safflower (Carthamus tinctorius L.) cotyledons yielded unesterified fatty acids as the predominant product of fatty-acid synthesis from [1-14C]acetate. Exogenous reduced pyridine nucleotides were not required for this synthesis, but [1-14C]acetate incorporation was absolutely dependent on addition of ATP. Linseed (Linum usitatissimum L.) cotyledons are green during development and plastids isolated from them resembled leaf chloroplasts with developed grana. In contrast to the safflower plastids, those from linseed were able to carry out fatty-acid synthesis at low irradiances without the addition of either pyridine nucleotides or ATP. Intact linseed cotyledons were capable of net photosynthesis at rates up to 95 μmol·mg-1 chlorophyll·h-1. However, the low-light environment inside the linseed capsule (approx. 15% of external) means that photosynthesis will not contribute appreciably to the carbon economy of the developing seed and its main role may be to supply cofactors for fatty-acid synthesis.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 80 (1990), S. 234-240 
    ISSN: 1432-2242
    Keywords: Erucic acid ; Fatty acid desaturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A diverse collection of mutants of Arabidopsis with altered seed lipid compositions was isolated by determining the fatty acid composition of samples of seed from 3,000 mutagenized lines. A series of mutations was identified that caused deficiencies in the elongation of 18∶1 to 20∶1, desaturation of 18∶1 to 18∶2, and desaturation of 18∶2 to 18∶3. In each of these cases the wild type exhibited incomplete dominance over the mutant allele. These results, along with results from earlier studies, point to a major influence of gene dosage in determining the fatty acid composition of seed lipids. A mutation was also isolated that resulted in increased accumulation of 18∶3. On the basis of the effects on fatty acid composition, the nature of the biochemical lesion in three of the mutants could be tentatively attributed to deficiencies in activities of specific enzymes. The other mutant classes had relatively less pronounced changes in fatty acid composition. These mutants may represent alterations in genes that regulate lipid metabolism or seed development. The availability of the mutants should provide new opportunities to investigate the mechanisms that control seed lipid fatty acid composition.
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  • 4
    Publication Date: 1985-01-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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