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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of metamorphic geology 13 (1995), S. 0 
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Abstract Five whole-rock 40Ar/39Ar plateau ages from low-grade sectors of the Sambagawa belt (Besshi nappe complex) range between 87 and 97 Ma. Two whole-rock phyllite samples from the Mikabu greenstone belt record well-defined 40Ar/39Ar plateau ages of 96 and 98 Ma. Together these ages suggest that a high-pressure metamorphism occurred in both the Sambagawa and Mikabu belts at c. 90–100 Ma. The northern Chichibu sub-belt may consist of several distinct geochronological units because metamorphic ages increase systematically from north (c. 110 Ma) to south (c. 215 Ma). The northern Chichibu sub-belt is correlated with the Kuma nappe complex (Sambagawa belt). Two whole-rock phyllite samples from the Kurosegawa terrane display markedly older metamorphic ages than either the Sambagawa or the Chichibu belts.Accretion of Sambagawa-Chichibu protoliths began prior to the middle Jurrasic. Depositional ages decrease from middle Jurassic (Kuma-Chichibu nappe complex) to c. 100 Ma (Oboke nappe complex) toward lower tectonostratigraphic units. The ages of metamorphic culmination also decrease from upper to lower tectonostratigraphic units. The Kurosegawa belt and the geological units to the south belong to distinctly different terrances than the Sambagawa-Chichibu belts. These have been juxtaposed as a result of transcurrent faulting during the Cretaceous.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: This study reports the occurrence of spontaneous hybridization between Odontesthes bonariensis (Valenciennes, 1835) and Patagonina hatcheri (Eigenmann, 1909), two South American freshwater atherinids, while adults of both species were being held in a communal laboratory tank, and describes suitable genetic markers for distinction of both species and their hybrids. Combined allozyme and mitochondrial DNA restriction length polymorphism analyses of offspring from three spawnings in the communal tanks confirmed the presence of hybrids along with purebreds in each spawning. Hybrids of an O. bonariensis mother X a P. hatcheri father were produced along purebred O. bonariensis in one occasion, whereas hybrids of P. hatcheri mothers X O. bonariensis fathers were produced along purebred P. hatcheri in two occasions. Three enzyme systems (coded by loci LDH-B*, PGM*, and CK*) out of 15 (20 loci) screened allowed distinction of species and their hybrids, but the usefulness of LDH-B* for field work may be limited by intraspecific polymorphism. Eight restriction enzymes (Apa I, Bgl II, Eco RI, Eco RV, Eco T22I, Hinc II, Hind III, and Pvu II) among 11 produced diagnostic restriction fragments applicable for species (motherhood) distinction. The relevance of these findings for the management of natural genetic resources and the aquaculture of these two species is discussed.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 51 (1997), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Probes were cloned, characterized, and developed for all regions of the mitochondrial DNA (mtDNA) of pejerrey Odontesthes bonariensis to provide the basis for the study of genetic diversity of South American atherinopsinii and to enable species identification from small amounts of tissue. The mtDNA was extracted from liver and cleaved with Eco RI, producing four fragments (7.4, 3.4, 3.1 and 2.9 kb) which were cloned using pUC118 plasmid vectors. Sequence analysis from both ends of the fragments showed that they encode tRNA (Asp, Phe, and Ser-TGA), 12 S rRNA, cytochrome oxidase (CO) II, NADH 4, 5, and 6, and the D-loop, and that the relative positions of these genes are identical to those in the mtDNA of other teleosts. A comparison of homology with carp mtDNA nucleotide sequences revealed that tRNA (Phe and Ser-TGA) and CO II were relatively conserved, whereas the D-loop region was highly divergent. The cloned mtDNA probes detected mtDNA fragments from about 800 ng of total DNA extracted from liver, muscle, and single embryos of O. bonariensis, and were effective for restriction length fragment polymorphism (RFLP) analysis of Patagonina hatcheri, the most distant atherinopsine relative of pejerrey. The cloned mtDNA probes may be useful for the analysis of genetic diversity and non-destructive species identification, including the examination of eggs, larvae and juveniles. The mtDNA sequences reported here provide the basis for the design of primers for PCR-based RFLP analysis.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 46 (1981), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A synthetic extract, prepared from 44 pure chemicals on the basis of the analytical data on the leg meat extract of the boiled snow crab, Chionoecetes opilio, satisfactorily reproduced the taste of the natural extract except for a slight lack of body and mildness. A series of taste panel assessments on synthetic extracts prepared by omitting or adding extractive component(s) were carried out by a triangle difference test and changes in taste profile were assessed. Only 12 components, Ala, Arg, Glu, Gly, glycine betaine, AMP, CMP, GMP, Na+, K+, Cl−, and PO43-, were found to contribute more or less toward producing the characteristic taste of the snow crab.
    Type of Medium: Electronic Resource
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