ALBERT

Notes: Seven pairs of near-isogenic lines (glandless vs. glanded) and the recurrent parents were used to determine the effects of the dominant glandless gene from ‘Hai 1’(Gossypium barbadense) on agronomic, fibre, and seed characters in Upland cotton, Gossypium hirsutum, backgrounds. The results showed that there were no apparent linkage associations of the glandless gene on most agronomic, fibre and seed characters of Upland cotton, except for seed quality. The glandless line derived from ‘Liaomian 7’had significantly more protein (489.6 g/kg), and that from H237 had significantly more oil (362.4 g/kg) and had the largest oil index (2.70 g) and protein index (3.03 g). The gossypol content of seed in dominant glandless lines in Upland cotton was very low (〈0.04 g/kg). Therefore, it is suggested that the glandless gene can play an important role in breeding glandless or low seed-gossypol Upland cotton cultivars.

Notes: The acidic ether fraction of methanol extracts from Cyperus rotundus L. (purple nutsedge) tubers contained inhibitory substances described hereafter as inhibitor β. Inhibitor β inhibited sprouting of excised C. rotundus buds and also the elongation of wheat coleoptiles. The inhibition of bud sprouting by inhibitor β was reversed by the application of N-6-benzyl adenine (BA). Applications of abscisic acid (ABA) also inhibited sprouting of the excised buds and this was similarly reversed by BA applications. Chromatographic evidence suggested that inhibitor β mainly consisted of phenolic materials and possibly ABA as a minor component. The role of BA in enhancing sprouting of C rotundus tubers is discussed.Inhibition des bourgeons de bulbes de Cyperus rotundus L. par I'inhibiteur β ou l'acide abscissique et réversion de ces effects par la N-6-benzyl adénineLa fraction éther acide d'extraits, par le méthanol. de bulbes de Cyperus rotundus L. contient des substances inhibitrices decrites ci-après comme I'inhibiteur β. Cet inhibiteur β a inhibé la germination de bulbes isolés de C. rotundus ainsi que l'élongation de coléoptiles de blé. L'inhibition de la germination des bourgeons par I'inhibiteur βété renversée par l'application de la N-6-benzyl adénine (B.A.). Des applications d'acide abscissique (ABA) ont également inhibé la germination de bourgeons isolés et cet effet a été lui aussi renversé par des applications de B.A. Les chromatogrammes suggèrent que l'inhibiteur β est principalement constitué par des composés phénoliques et peut-être par I'ABA comme composant mineur. Le rôle de la B.A. dans l'inhibition de la germination des bulbes de C. rotundus est discuté.Knospenhemmung der Knollen von Cyperus rotundus L. durch Hemmstoff β oder Abscisinsäure und die Umkehrung dieser Effekte durch N-6-BenzylmleninDie saure Fraktion aus methanolischen Extrakten von Cyperus rotundus L.-Knollen enthielten Hemmstoffe, die im folgenden als Hemmstoff β bezeichnet werden. Hemmstoff β hemmte das Sprossen isolierter C. rotundus-Knospen und die Streckung von Weizenkoleoptilen. Die Hemmung der Knospensprossung durch Hemmstoff β wurde durch An-wendung von N-6-Benzyladenin (BA) aufgehoben. Abscisinsäure (ABA) hemmte auch das Sprossen isolierter Knospen; durch BA wurde es auf ähnliche Weise aufgehoben. Der chromatographische Befund deutet darauf hin. Daß Hemmstoff β sich hauptsächlich aus phenolischen Verbindungen zusammensetzt und möglicherweise in gering-em Unfang ABA enthält. Die Rolle von BA auf die Förderung der Keimung von C. rotundus-Knollen wird diskutiert.

Notes: Brucella melitensis 16M is a Gram-negative α2-proteobacterium responsible for abortion in goats and for Malta fever in humans. This facultative intracellular pathogen invades into and survives within both professional and non-professional phagocytes. Signature-tagged mutagenesis (STM) was used to identify genes required for the in vivo pathogenesis of Brucella. A library of transposon mutants was screened in a murine infection model. Out of 672 mutants screened, 20 were not recovered after a 5 day passage in BALB/c mice. The attenuation of 18 mutants was confirmed using an in vivo competition assay against the wild-type strain. The 18 mutants were characterized further for their ability to replicate in murine macrophages and in HeLa cells. The sequences disrupted by the transposon in the mutants have homology to genes coding for proteins of different functional classes: transport, amino acid and DNA metabolism, transcriptional regulation, peptidoglycan synthesis, a chaperone-like protein and proteins of unknown function. The mutants selected in this study provide new insights into the molecular basis of Brucella virulence.

Notes: Invasive pulmonary aspergillosis, usually caused by Aspergillus fumigatus, is a life-threatening condition of immunosuppressed patients. We have created a mutant strain of this fungus that lacks an extracellular alkaline protease (AFAlp). This was accomplished by transformation of A. fumigatus with a plasmid containing a selectable marker for hygromycin B resistance, and a 504 bp segment of the AFAlp gene, obtained by polymerase-chain-reaction-based amplification of A. fumigatus genomic DNA. Approximately 25% of transformants resulted from disruption of the AFAlp gene. SDS-polyacrylamide gel etectrophoresis of proteins from the culture filtrate of a strain carrying the AFAlp gene disruption showed that it lacked a major protein of 33 kDa. Furthermore, in contrast to the culture filtrate from wild-type cells, the mutant had undetectable activity on azocollagen and elastin-Congo red, over a broad pH range. This shows that AFAlp accounts for most, if not all, of the extracellular elastinolytic activity of A. fumigatus, and that the mutant strain will be useful in assessing the role of AFAlp in pathogenicity.

Notes: Papaya seeds, which constitute 22% of the waste product from the papaya puree industry in Hawaii, were characterized in a preliminary study to evaluate their possible utilization. Oil was extracted from the seeds, and its fatty acid composition determined. Crude protein and crude fiber contents of the defatted meal were especially high, 40.0% and 48.9%. Benzylisothiocyanate content in the seed oil was 0.56% (w/w), and the benzylglucosinolate content in the seed meal was 1.0 (w/w).

Notes: The effects of frozen storage and packaging methods on the oxidation of fatty acids and rancidity development in fresh-water prawn Macrobrachium rosenbergii were studied. The lipids of these fresh prawns contained 23% saturated, 46% monounsaturated, and 31% polyunsaturated fatty acids. The fatty acids, especially the unsaturated ones decreased during frozen storage for 6 months at -18°C, regardless of the packaging procedure employed. No objectionable rancid flavor was detected in these prawns during the 6-month frozen storage study.