ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Ion exchange whey protein concentrate (IEWPC) was produced from acid whey using Spherosil-S, a microporous silica bead cation exchange medium. About 80% of the total whey proteins were recovered by the ion exchange process. The composition of IEWPC was 63 - 66% protein, 1 - 2% lactose, 7 - 9% milkfat and 20 - 22% ash. Solubility, PAGE, reverse phase HPLC and Sephadex gel frltration results confirmed that the proteins in IEWPC had undergone substantial denaturation during their isolation. Several major problems encountered during the study were: generation of large volumes of column effluent, the need for concentration of column protein elutate fractions by ultrafiltration, column fouling and loss of protein solubility and functionality.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1985.tb13756.x
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