ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
The effect of salt stress on polypeptide and translatable mRNA levels was examined in roots of barley (Hordeum vulgare L. cv. CM 72). A salt-shock time course (200 mM NaCl added to the nutrient solution of 5-day old seedlings for up to 24 h) and a 6-day salt treatment (seedlings grown in nutrient solution containing 200 mM NaCl for 6 days) were compared. Roots of intact seedlings were labeled in vivo with [35S]-methionine. Poly(A)+ RNA was isolated and assayed in an in vitro translation system. The changes in the levels of the labeled polypeptides and translation products were analyzed following separation on two-dimensional polyacrylamide gels. Over the salt-shock time course, the majority of the changes in polypeptides and translation products were quantitative, as were the changes at 6 days. Qualitative changes occurred during the salt-shock time course, but were not observed at 6 days. The levels of polypeptides and translation products differed at each point of the salt-shock time course and at 6 days. In addition, changes were observed at the shortest time points examined, indicating a rapid response to salt.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1990.tb08726.x
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