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  • 1
    Electronic Resource
    Electronic Resource
    Microsporidiosis in HIV-Positive Children in Madrid (Spain). (1997)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 44 (1997), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A prospective study was carried out to determine the prevalence rates of microsporidiosis and other enteroparasites in HIV-positive children in the Madrid area. HIV-positive pediatric patients from three hospitals were entrolled in the study. A total of 293 samples (158 stool and 127 urine) were collected from 83 children whose mean age was 6.3 years and hed a mean CD4 count of 504.7/mm3 (range 1-2,220/mm3), 48 of whom suffered diarrhea at the time of the study. Microsporidia indentification was investigated in stool and unrine samples using Weber's chromotrope-based strain, IIF and PCR species-specific tests. Enteric parasites were identified in 32.5% of the children. Cryptosporidium sp. was the most common parasite encuntered (14.4%), followed by Blastocytis sp. (9.6%) and Giardia duodenalis (8.4%). Microsoridia was only found in the stools of one child (1.2% of total and 2% of those with diarrhea) and Enterocytozoon bieneusi was demonstrated by PCR. The patient was 10 years old, Presented non-chronic diarrhea and his CD4 count was 298/mm3. These date differ from those previously reported by us in HIV-positive adults (13.9%) in the same area, although this group showed more severely depressed CD4 lymphocyte counts than children. New epidemiological studies should be carried out to elucidate whether additional risk factors exist betwen these groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1997.tb05798.x
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  • 2
    Electronic Resource
    Electronic Resource
    Improvement of the Sensory Properties of Dry Fermented Sausages by the Superficial Inoculation and/or the Addition of Intracellular Extracts of Mucor racemosus (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 65 (2000), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : The effect of inoculation of the “salchichón” (dry fermented sausage) surface with an atoxigenic, proteolytic, and lipolytic strain of Mucor racemosus and/or the addition of a intracellular extract of the same mold on the ripening process was studied. Four batches of salchichón were manufactured: control, superficially inoculated, added with intracellular extract, and a combination of both treatments. Superficial mold modified the amino and free fatty acid contents, volatile compounds, and sensory properties. Intracellular extract degraded free amino acids, producing a rise in ammonia and volatile compounds. Both treatments combined improved significantly the sensory properties, which were given scores higher than control sausages at the end of ripening.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2000.tb16081.x
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  • 3
    Electronic Resource
    Electronic Resource
    Innocuous Alterations in Preserved Onions, Allium cepa L. (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 46 (1981), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In the outer layers of pickled or canned onions there appears, at times, yellow colorations or deposits of yellow substances. The pigment has been identified as quercetin, a normal component of onion skins; this alteration is furthermore found to be totally innocuous.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1981.tb04536.x
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  • 4
    Electronic Resource
    Electronic Resource
    Two-dimensional geoid modelling: some remarks on Chapman's algorithm (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 127 (1996), S. 0 
    Details
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: An analytical solution and its application to compute 2-D geoid anomalies, based on Brun's formula (Heiskanen & Moritz 1967), was developed by Chapman (1979). In this paper we present the derived solution from a thorough review of Chapman's algorithm. Apart from some typographical errors in the mathematical expressions, we have found that some of the precautions given by the author are not necessary when the algorithm is applied, in particular those regarding the unit system, topography and density contrast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-246X.1996.tb04739.x
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  • 5
    Electronic Resource
    Electronic Resource
    Occurrence of Bacillus species on evergreen oak leaves (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 14 (1994), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Thirty-five spore-forming bacilli were isolated from evergreen oak leaves at four stages (one-year-old and two-year-old leaves, upper layer and underlayer little leaves) and over the four seasons within one year. These isolates, plus five reference strains, were characterized morphologically and physiologically by a total of 100 tests and genetically by DNA/DNA hybridization. Phenotypic similarities of all strains were determined by numerical taxonomy, with the unweighted average linkage (UPGMA) algorithm and simple matching (SSM) and Jaccard (SJ) coefficients used as measures for similarity. Three groups (A to C) were defined at a similarity level of 71% (SSM) or 57% (SJ). They contained leaf isolates phenotypically related to Bacillus cereus, B. pumilus and B. circulans species respectively. The majority of the leaf isolates were asigned to B. cereus (34%) and B. pumilus (63%). DNA/DNA hybridization also discriminated three groups (genomic groups 1, 2 and unclassified strains) which presented a good correlation with the numerical analysis. Yet, DNA/DNA hybridization grouping revealed a higher degree of discrimination by defining four subgroups (1a, 1b, 2a, and 2b). Genomic subgroup 1a contained leaf isolates belonging to the B. cereus species; isolates from genomic subgroup 1b belonged to B. mycoides species and isolates from genomic subgroup 2a belonged to B. pumilus species. Subgroup 2b consisted of a new genomic subspecies of B. pumilus which exhibited a degree of homology ranging from 53 to 64% with the B. pumilus type strain and a coefficient of divergence (ΔTm) ranging from 5.5 to 7°C. The different genomic groups presented different substrate metabolism capacities and a different spatial distribution on evergreen oak leaves. B. cereus strains (group 1) were predominantly located on litter leaves whereas B. pumilus strain (group 2) were found on the phyllosphere. In contrast with group 1, group 2 was able to metabolize some sugars and pectin, while group 1 isolates were able to hydrolyze starch and glycogen. Thus, our hypothesis is that group 1 succeeds group 2 when the leaves are littered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1994.tb00118.x
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  • 6
    Electronic Resource
    Electronic Resource
    Marine Amoebae from Waters of Northwest Spain, with Comments on a Potentially Pathogenic Euryhaline Species (1989)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 36 (1989), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Of 17 species of free-living amoebae identified in various samples of salt water, only 1. Acanthamoeba polyphaga, is known to be a potential pathogen. While no deaths occurred when laboratory animals were inoculated with A. polyphaga to test for pathogenicity, the protozoa were present in the brain, liver and lungs of some but not all of the animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1989.tb05356.x
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular cloning of a Salmonella typhi LT-like enterotoxin gene (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 2 (1988), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Diarrhoea is a common event during typhoid fever; nevertheless, the possible participation of a diarrhoea-inducing enterotoxin has not been described (Roy et al., 1985). Recombinant bacteriophage λ FDC1 was isolated from a genomic library of Salmonella typhi, the causal agent of typhoid fever, by screening with a probe for the B subunit gene of the heat-labile, cholera-like, Escherichia coli enterotoxin (LT). λ FDC1 codes for an enterotoxin that causes secretion in rat ileal loops, that elongates Chinese hamster ovary (CHO) cells, that is recognized by antibodies against LT, and does not bind in vitroto ganglioside GM1. These results should allow further studies towards elucidating a possible role for the S. typhi enterotoxin in the pathogenesis of typhoid fever.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1988.tb00094.x
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  • 8
    Electronic Resource
    Electronic Resource
    Partial deletion of the Rhizobium phaseoli CFN23 symbiotic plasmid implies a concomitant amplification of plasmid DNA sequences (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Rhizobium leguminosarum biovar phaseol CFN23 loses its ability to nodulate beans at a high frequency because of a deletion of part of its symbiotic (pSym) plasmid (Soberón-Chávez et al., 1986). We report here that at least 80kb of pSym are deleted upon loss of the symbiotic phenotype; the deletion removes the nitrogenase structural nifHDK and the common nod ABC genes. The size of the deleted pSym is not reduced, since it is accompanied by an amplification of other pSym plasmid sequences. This genetic rearrangement is similar to the deletion and amplification of yeast mitochondrial DNA leading to‘petite’mutations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb01829.x
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  • 9
    Electronic Resource
    Electronic Resource
    Distribution and remobilization of symbiotically fixed nitrogen in soybean (Glycine max) (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 65 (1985), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Partitioning of nitrogen by soybeans (Glycine max L. Merr. cv. Hodgson) grown in natural conditions was studied by successive exposures of root systems to 15N2 and periodical measurements of 15N distribution. Nitrogen derived from the atmosphere was mainly found in the aerial parts of the plants, and the stage of development exerted a strong influence on the initial 15N distribution (measured one week after incorporation). Until day 69 after sowing, leaf blades contained 47 to 57% of the fixed N. After that, reproductive structures attracted increasing proportions, 10 to 60% between days 69 and 92. Around day 82, stems and petioles stored up to 30% of the newly fixed N. During pod development and pod filling and until maturity, fixed N was remobilized from vegetative tissues and pod walls to seeds. These transfers first concerned the newly incorporated N, but at maturity 80 to 90% of the total was recovered in the seeds. The high mobility of N originating from the atmosphere as compared to that coming from the soil (vegetative tissues exported only 50% of their total N) seems to indicate that fixed N was at least partially integrated in a special pool. This was certainly the case at the later stage of N2 fixation, when a large portion of fixed N accumulated in the stems and petioles, probably in the form of storage compounds such as ureides for later transfer to the developing seeds. Further research is needed in order to investigate the nature and role of this pool in the nitrogen nutrition of soybeans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1985.tb02396.x
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  • 10
    Electronic Resource
    Electronic Resource
    Methods to Detect the Occurrence of Various Indicator Bacteria on the Surface of Retail Poultry in Spain (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Contamination levels (cfu/g and cfu/cm2) of indicator microorganisms in retail broiler chicken carcasses in León (Spain) were investigated. Counts (log10 cfu/g) were 5.19, 3.04, 2.73, 3.38, and 3.16 for total aerobic counts (TAC), Enterobacteriaceae, coliforms determined by the standard VRBA method (coliforms-VRBA), coliforms determined by the Hydrophobic Grid Membrane Filter method (coliforms-HGMF), and Escherichia coli (HGMF method), respectively. These values fit into the microbiological criteria for poultry meat consulted. A low correlation coefficient was found between TAC and Enterobacteriaceae counts (r = 0.308; P = 0.053) and between coliforms-VRBA and coliforms-HGMF counts (r = 0.398; P= 0.048). The determination method had a significant influence on the coliform counts obtained. All broiler chicken carcasses harbored E. coli biotype I. E. coli biotype II was detected in 20% of the samples. The HGMF method was not completely specific for detecting E. coli since 11.25% of false positive colonies were found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2002.tb10673.x
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