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  • Blackwell Publishing Ltd  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 43 (1978), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An oleoresin-cellulose model was used for studying the interacting processes which influence carotene bleaching in powdered paprika. Special attention was given to the effects of water activity and the addition of aqueous pepper extracts, copper and ascorbic acid. The stability of carotenoids in the model system increased slightly with increasing water activity, but the effect of moisture become considerable when the system also included ascorbic acid and copper sulfate. When aw in powdered paprika exceeded 0.32, carotenoid bleaching proceeded in three stages. After an initial bleaching, there was a prolonged period of stability, after which oxidation resumed. The intermediate period of stability was absent in the dry product. Results obtained with the oleoresin-cellulose model showed that the three-stage carotenoid bleaching at high aw values was characteristic of the combined action of copper and ascorbic acid. It is concluded that these two materials, by becoming soluble at high water activities, play an important part in the stabilization of carotenoids in powdered paprika. Under conditions of high water activity, the prooxidant effect of a water-soluble protein is overcome by the marked antioxidant action of the ascorbic acid-copper system. However, in the dry product the decisive factor in carotenoid bleaching is the proteinous fraction which was previously shown to have peroxidase activity.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 54 (1982), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Factors relating to photo-oxidative damage in tomatoes were investigated during maturation of the fruit and upon induction of sunscald. Superoxide dismutase (SOD) activity passed through a minimum at the mature-green and breaker stages of ripening and availability of zinc and copper did not appear to be a limiting factor in the synthesis of the enzyme. Iron levels were maximal and total carotenoid concentrations were lowest during the same mature-green and breaker stages of maturation, while chlorophyll was starting to decrease but was still present in large amounts. Peroxidase activity decreased steadily during ripening. Artificial induction of tolerance to photodynamic damage by controlled heat treatment was accompanied by an increase in SOD activity, while carotenoid levels and peroxidase activity did not change. These findings support the thesis that the previously reported susceptibility of tomatoes to photodynamic damage, i.e. sunscald, during the mature-green and breaker stages of maturation is related to enhanced formation of superoxide ions, at a time when chloroplast structure begins to break down. SOD, by scavenging the superoxide, appears to supplement the protective action of carotenoids against photo-oxidative injury.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 42 (1977), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The activity of aqueous extracts from red pepper fruits (Capsicum annuum L.) toward the oxidation of carotene presence of linoleate was measured by a colorimetric method. Highest activities were achieved by using as extractant distilled water or Tris buffer at pH 7.0, whereas solubilization of carotene-bleaching factors by Triton X-100 or preparation of acetone powder from pepper fruit extracts resulted in losses in carotene-oxidizing activity. After dialysis, ammonium sulfate precipitation and chromatographic purification on Ecteola and Sepharose 6-B, a proteinous carotene-oxidizing fraction was obtained which exhibited properties similar to those of a peroxidase, i.e., high specific activity toward guaiacol oxidation in the presence of hydrogen peroxide, precipitation with ammonium sulfate between 60 and 95% saturation, change in spectral absorbance upon addition of cyanide, inhibition of carotene bleaching activity by KCN, and typical changes in carotene-bleaching activity with pH and protein concentration.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 42 (1977), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The interacting effects of ascorbic acid and metal ions on carotene oxidation were studied in an aqueous carotene-linoleate solution at pH 7. Ascorbic acid at concentrations up to 10—3 M was a prooxidant. Fe3+ and, to a lesser extent Co2+, acted synergistically with ascorbic acid, the prooxidant effect increasing with metal concentration. Cu2+ formed a prooxidant system with ascorbic acid only at low metal concentration, but as the copper concentration was raised, inversion of activity occurred and the copper-ascorbic acid system exerted a stabilizing action on carotene. Prooxidant effects were enhanced and antioxidant effects weakened in the presence of added lmoleate hydroperoxides. The latter were unstable in the presence of ascorbic acid and especially ascorbic acid + Cu2+. Ascorbic acid itself became unstable in the presence of Cu2+. Oxygen depletion, brought about by the rapid oxidation of ascorbic acid, may be partly responsible for the carotene-stabilizing effect of the Cu*+-ascorbic acid couple. It is postulated that additional stabilization results from the radical-scavenging properties of copper or of a copper chelate formed by ascorbic and/or dehydro-ascorbic acid.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 43 (1978), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The carotene-bleaching activities of ascorbic acid and copper were investigated in a solid model consisting of carotene and linoleic acid adsorbed on powdered cellulose. The effects depended on the water activity (aw) of the system. Ascorbic acid in the presence of Cu++ had very little activity in the dry model but displayed increasing bleaching effects as the aw value of the system was raised. However this bleaching activity in the moist model reached a maximum as the ascorbic acid concentration rose to about 1 μmol/g cellulose, or 1.4 μmol ascorbic acid/mmol linoleic acid. At higher concentrations of ascorbic acid, the bleaching activity decreased and was eventually converted into an antioxidant effect. A peroxidase-like protein extracted from pepper was also tested in the present model and found to exhibit prooxidant activity, which was little affected by changes in aw. The antioxidant system composed of copper and ascorbic acid at high concentrations was able to overcome the pro-oxidant effect of peroxidase.
    Type of Medium: Electronic Resource
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