ISSN:
1749-6632
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Natural Sciences in General
Notes:
Ferritin-conjugated antibodies and ferritin-conjugated plant agglutinins can be used as specific stains to localize macromolecular antigens and specific saccharide residues, respectively, on membranes. In conjunction with a new procedure for preparing membrane specimens by cytolysis at an air-water interface, specific ferritin-conjugated antibodies have been used to determine the distribution of the Rh0(D) antigen on human erythrocyte membranes, and of the H-2 histocompatibility alloantigens on murine erythrocytes. Over a period of time these distributions are random, lending support to the lipid-globular protein mosaic model of membrane structure. With ferritin-conjugated plant agglutinins, it has been found, for several types of saccharide residues and several varieties of plasma membranes, that the membrane-bound oligosaccharides are exclusively located on the outer surfaces of the membranes; none is found on the inner cytoplasmic surface. These results strongly suggest that the glycoproteins of membranes do not rotate from the outer to the inner surface at a significant rate under physiological conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1749-6632.1972.tb54817.x
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