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  • Articles  (3)
  • Blackwell Publishing Ltd  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Relationships in pineapple by random amplified polymorphic DNA (RAPD) analysis (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Random amplified polymorphic DNA (RAPD) analysis was applied to eight commercial cultivars of pineapple, two intergroup hybrids and two wild species. Morphologically, pineapple is divided into the Cayenne, Queen, Spanish, Maipure and Abacaxi groups. Members of the first three groups have been analysed in this study. The cultivars ‘Tradsithong’, ‘Phuket’, ‘Sawee’ and ‘Tainan’, with spiny leaves, form the Queen group. In ‘Pattavia’, ‘Nanglae’ and ‘Petburi no. 2’ (Cayenne group), spines are confined to the leaf tips. ‘Intrachitdang’ is normally placed in the Spanish group, which is morphologically similar to the Queen group, but with inferior quality fruit. DNA amplification products were compared from 16 arbitrary 10-mer primers from which a dendrogram was constructed. The results confirmed morphological classifications for seven of the eight commercial cultivars, with the Queen and Cayenne groups as separate clusters. However, the cv. ‘Intrachitdang’ was more closely related to the Cayenne group. Two hybrids from reciprocal Cayenne × Queen group crosses, were more closely allied to the Queen group. The two wild species were outside the groups. RAPD analysis can be exploited to investigate relationships within pineapple germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00606.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic diversity in mung bean germplasm revealed by RAPD markers (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 117 (1998), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Knowledge of the genetic relationships among landraces is useful to gene bank managers because it permits a better organization of the crop's gene pool management, more efficient sampling of the available germplasm resources and better access to useful genetic variation for breeders. Genetic diversity of 19 landraces of the cultivated mung bean, Vigna radiate, and three weedy and wild relatives including Vigna mungo, Vigna luteola and Vigna radiate var. sublobata, was investigated at the DNA level with the random amplified polymorphic DNA (RAPD) procedure. Sixty random decamer primers were employed in amplification reactions; 28 of these were informative and yielded 246 bands, of which 229 were polymorphic with a mean of 8.2 bands per primer. A genetic distance matrix based on Nei and Li coefficient was converted to a dendrogram and a two-dimensional plot using multidimensional scaling (MDS). The accessions studied were separated into three main clusters, which included V. radiate landraces, V. mungo and V. luteola, respectively. The variation of this cluster supports the view that the genetic distance of V. mungo and V. luteola varies considerably from the accession VO2955 (V. radiata). The multidimensional scaling plot confirmed that V. mungo, V. luteola and most of the accessions of V. radiata formed distinct clusters with no overlap, and two mung bean accessions (PI177493 and VO4134–1 from Turkey and India, respectively) were genetically distant from other V. radiata landraces. V. radiata and V. mungo are positioned in separate botanical species and V. radiata var. sublobata is classified within other V. radiata landraces. Based on the limited range of accessions tested, the approach holds promise for the classification of mung bean germplasm, identification of mung bean landraces and applications of molecular markers to mung bean breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1998.tb01976.x
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  • 3
    Electronic Resource
    Electronic Resource
    Hybridization, Through Culture of Embryos and Immature Seeds, of a Range of Tomato Cultivars with a Tomato Somatic Hybrid [Lycopersicon esculentum (+) L. peruvianum]: Emergence of a Possible New Marker Gene for Tomato Breeding (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 111 (1993), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two hexaploid somatic hybrids [Sh; L. esculentum (+) L. Peruvianum] accessions 6 and 18 were back-crossed with two diploid L. Esculentum cultivars ‘Moneymaker’ (mm) and ‘pusa Ruby’ (pr). Twenty-two plants of the bc2 generation were produced by backcrossing 7 bcl plants (mm x sh, 6, 18) with five tomato cultivars. Fourteen of the bc2 plants were self-fertile, five produced anther cones with anthocyanin pigmentation not present in the parents. A bc3 generation was developed by crossing the four cultivars as female parent with three bc2 generation plants. The bc3 progeny derived from one pollen parent plant were produced without the need to culture immature seeds. They segregated with respect to pigmented anther cones and were self-fertile. The anther cone pigmentation of the pollen parent plant was associated with increased seed set, greater fruit size and an orange-red fruit colour. These features were transmitted to the fertile bc3 generation. Conversely, bc3 offspring involving the other two parent plants were only recovered by culture of immature seeds. The recovery of diploid plants in BCl and self-fertility in BC2 resulted in almost total recovery of the tomato cultivar characteristics (fruit size, colour and number of seeds) by BC3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1993.tb00642.x
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