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  • photosynthesis  (31)
  • Springer  (31)
  • Blackwell Publishing Ltd
  • Molecular Diversity Preservation International (MDPI)
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  • Springer  (31)
  • Blackwell Publishing Ltd
  • Molecular Diversity Preservation International (MDPI)
  • Wiley-Blackwell  (1)
  • 1
    ISSN: 1573-5036
    Keywords: atmospheric CO2 ; belowground production ; labile soil C ; microbial biomass ; N mineralization ; photosynthesis ; positive feedback
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We tested a conceptual model describing the influence of elevated atmospheric CO2 on plant production, soil microorganisms, and the cycling of C and N in the plant-soil system. Our model is based on the observation that in nutrient-poor soils, plants (C3) grown in an elevated CO2 atmosphere often increase production and allocation to belowground structures. We predicted that greater belowground C inputs at elevated CO2 should elicit an increase in soil microbial biomass and increased rates of organic matter turnover and nitrogen availability. We measured photosynthesis, biomass production, and C allocation of Populus grandidentata Michx. grown in nutrient-poor soil for one field season at ambient and twice-ambient (i.e., elevated) atmospheric CO2 concentrations. Plants were grown in a sandy subsurface soil i) at ambient CO2 with no open top chamber, ii) at ambient CO2 in an open top chamber, and iii) at twice-ambient CO2 in an open top chamber. Plants were fertilized with 4.5 g N m−2 over a 47 d period midway through the growing season. Following 152 d of growth, we quantified microbial biomass and the availabilities of C and N in rhizosphere and bulk soil. We tested for a significant CO2 effect on plant growth and soil C and N dynamics by comparing the means of the chambered ambient and chambered elevated CO2 treatments. Rates of photosynthesis in plants grown at elevated CO2 were significantly greater than those measured under ambient conditions. The number of roots, root length, and root length increment were also substantially greater at elevated CO2. Total and belowground biomass were significantly greater at elevated CO2. Under N-limited conditions, plants allocated 50–70% of their biomass to roots. Labile C in the rhizosphere of elevated-grown plants was significantly greater than that measured in the ambient treatments; there were no significant differences between labile C pools in the bulk soil of ambient and elevated-grown plants. Microbial biomass C was significantly greater in the rhizosphere and bulk soil of plants grown at elevated CO2 compared to that in the ambient treatment. Moreover, a short-term laboratory assay of N mineralization indicated that N availability was significantly greater in the bulk soil of the elevated-grown plants. Our results suggest that elevated atmospheric CO2 concentrations can have a positive feedback effect on soil C and N dynamics producing greater N availability. Experiments conducted for longer periods of time will be necessary to test the potential for negative feedback due to altered leaf litter chemistry. ei]{gnH}{fnLambers} ei]{gnA C}{fnBorstlap}
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  • 2
    ISSN: 1573-5079
    Keywords: energy transfer ; fluorescence kinetics ; photosynthesis ; Porphyridium cruentum ; spillover ; state transitions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Excitation energy distribution in Porphyridium cruentum in state 1 and state 2 was investigated by time resolved 77 K fluorescence emission spectroscopy. The fluorescence rise times of phycoerythrin, phycocyanin and allophycocyanin (in cells in state 1 and state 2) were very similar in contrast to the emission from chlorophyll a (Chl a) associated with the two photosystems. In state 2 photosystem II (PSII) Chl a fluorescence emission rose faster than the PSI Chl a emission and decayed more rapidly, and the converse was observed in state 1. These kinetic data support the concept of increased energy transfer from PSII Chl a to PSI Chl a in state 2 in P. cruentum.
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  • 3
    ISSN: 1573-5079
    Keywords: Chondrus crispus ; mitochondrial respiration ; Mehler reaction ; photorespiration ; photosynthesis ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Pour identifier la nature des réactions responsables de la prise d'oxygène sous lumière chez une macroalgue rouge Chondrus crispus Stackhouse (Rhodophyta, Gigartinales), les effets de quelques inhibiteurs, de l'intensité de la lumière et de la température ont été étudiés en combinaison avec des concentrations de CO2 élevées ou limitantes. Dans ce dernier cas, INH (2, 5 mM) ne modifie pas la prise d'oxygène sous lumière et ne provoque pas l'excrétion ni l'accumulation de glycolate. KCN réduit la vitesse de consommation d'O2 de 76% à CO2 limitant, de 43% à CO2 saturant et supprime pratiquement (à 95%) la production d'oxygène. Avec DCMU (5 μM) qui bloque totalement la chaîne des transporteurs d'électrons, une prise d'oxygène résiduelle est observée de 3,0±0,6 μmol O2 h−1 g−1 P.F. quelle que soit la concentration de CO2. Ajoutés à l'analyse de l'effet de la température ces résultats suggèrent: 1) La respiration mitochondriale et la réaction de Mehler sont actives aux deux concentrations de CO2. 2) La RuBP oxygénase contribue pour 45%, au plus, et la réaction de Mehler pour 20%, au moins, à la prise d'oxygène sous lumière à CO2 limitant.
    Notes: Abstract The nature of the different processes of O2 uptake involved in the light in the red macroalga Chondrus crispus Stackhouse (Rhodophyta, Gigartinales) was investigated. At limiting CO2, INH (2.5 mM) did not alter the O2 uptake rate. Glycolate was not excreted and did not accumulate within the cells. KCN reduced the rate of O2 uptake in the light by 76% at limiting CO2 and by 43% at saturating CO2, but caused 〉 95% inhibition of O2 evolution. DCMU (5 μM) totally blocked the photosynthetic electron transport chain, but allowed a residual O2 uptake of 3.0±0.6 μmol O2 .h−1.g−1 FW, irrespective of the CO2 concentration. In saturating CO2, a high light intensity pretreatment significantly stimulated the rate of O2 uptake compared to net O2 evolution, suggesting the persistence, in the light, of mitochondrial respiration. Irrespective of the CO2 concentration, the optimum temperature for O2 evolution was 17°C whereas dark O2 uptake increased linearly with temperature. In contrast, O2 uptake in the light showed an optimum at 17°C in limiting CO2, and 21–25° C in saturating CO2; its Q10 was 2.4 at limiting CO2, a value close to that of RuBP oxygenase, and 3.1 at saturating CO2, a value close to that of dark respiration. It is concluded that: 1) mitochondrial respiration and Mehler reaction are both involved at all CO2 concentrations, 2) RuBP oxygenase activity cannot account for more than 45%, and Mehler reaction for less than 20%, of the total O2 uptake observed in the light at limiting CO2.
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  • 4
    ISSN: 1573-5079
    Keywords: cytochrome c ; photosynthesis ; photosynthetic bacteria ; electron transport ; Chloroflexus aurantiacus ; green bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The membrane-bound photooxidizable cytochrome c-554 from Chloroflexus aurantiacus has been purified. The purified protein runs as a single heme staining band on SDS-PAGE with an apparent molecular mass of 43 000 daltons. An extinction coefficient of 28 ± 1 mM−1 cm−1 per heme at 554 nm was found for the dithionite-reduced protein. The potentiometric titration of the hemes takes place over an extended range, showing clearly that the protein does not contain a single heme in a well-defined site. The titration can be fit to a Nernst curve with midpoint potentials at 0, +120, +220 and +300 mV vs the standard hydrogen electrode. Pyridine hemochrome analysis combined with a Lowry protein assay and the SDS-PAGE molecular weight indicates that there are a minimum of three, and probably four hemes per peptide. Amino acid analysis shows 5 histidine residues and 29% hydrophobic residues in the protein. This cytochrome appears to be functionally similar to the bound cytochrome from Rhodopseudomonas viridis. Both cytochrome c-554 from C. aurantiacus and the four-heme cytochrome c-558-553 from R. viridis appear to act as direct electron donors to the special bacteriochlorophyll pair of the photosynthetic reaction center. They have a similar content of hydrophobic amino acids, but differ in isoelectric point, thermodynamic characteristics, spectral properties, and in their ability to be photooxidized at low temperature.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 23 (1990), S. 313-318 
    ISSN: 1573-5079
    Keywords: thylakoid membranes ; electron transfer ; photoacoustic spectroscopy ; energy storage ; photosynthesis ; plastoquinone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The photosynthetic energy storage yield of uncoupled thylakoid membranes was monitored by photoacoustic spectroscopy at various measuring beam intensities. The energy storage rate as evaluated by the half-saturation measuring beam intensity (i50) was inhibited by 3-(3,4-dichlorophenyl)-1,1 dimethylurea, by heat inactivation or by artificial electron acceptors specific for photosystem I or photosystem II; and was activated by electron donors to photosystem I. The reactions involving both photosystems were all characterized by a similar maximal energy storage yield of 16±2 percent. The data could be interpreted if we assumed that the energy storage elicited by the photosystems at 35 Hz is detected at the level of the plastoquinone pool.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 41 (1994), S. 175-180 
    ISSN: 1573-5079
    Keywords: Chlorobium ; Chloroflexus ; chlorophyll ; light-harvesting ; photosynthesis ; resonance Raman
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Resonance Raman experiments were performed on different green bacteria. With blue excitation, i.e. under Soret resonance or preresonance conditions, resonance Raman contributions were essentially arising from the chlorosome pigments. By comparing these spectra and those of isolated chlorosomes, it is possible to evaluate how the latter retain their native structure during the isolation procedures. The structure of bacteriochlorophyll oligomers in chlorosomes was interspecifically compared, in bacteriochlorophyllc- and bacteriochlorophylle- synthesising bacteria. It appears that interactions assumed by the 9-keto carbonyl group are identical inChlorobium limicola, Chlorobium tepidum, andChlorobium phaeobacteroides. In the latter strain, the 3-formyl carbonyl group of bacteriochlorophylle is kept free from intermolecular interactions. By contrast, resonance Raman spectra unambiguously indicate that the structure of bacteriochlorophyll oligomers is slightly different in chlorosomes fromChloroflexus auranticus, either isolated or in the whole bacteria.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 2 (1981), S. 213-223 
    ISSN: 1573-5079
    Keywords: photosynthesis ; photorespiration ; leaf development ; apple (Malus domestica Borkh.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A comprehensive developmental survey of leaf area, chlorophyll, photosynthetic rate, leaf resistance, transpiration ratio, CO2 compensation point and photorespiration was conducted in apple. The largest changes in each of the photosynthetic characteristics studied took place during the earliest stages of leaf development, coinciding with the period of greatest leaf expansion and chlorophyll synthesis. During early development, photosynthesis increased 5-fold, reaching a maximum rate of 40 mg CO2 dm-2 hr-1 at a leaf plastochron index (LPI) of 10. During this same period, leaf resistance, transpiration ratio, CO2 compensation point and mesophyll resistance decreased, while carboxylation efficiency increased. Two especially interesting aspects of the data discussed are simultaneous changes that occur at a LPI of 10 and 12 in all of the photosynthetic characteristics examined and an apparent decrease in photorespiration as leaves age. From our results it is clear that stage of leaf development is an important factor affecting the rate of photosynthesis and photorespiration.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 21 (1989), S. 151-159 
    ISSN: 1573-5079
    Keywords: photosynthesis ; stomatal conductance ; Theobroma cacao ; transpiration ; water stress ; water-use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of leaf water potential (Ψ) on net CO2 assimilation rate (A), stomatal conductance (g), transpiration (E) and water-use efficiency (WUE) was measured for three cultivars of cacao (Theobroma cacao L.) seedlings during three recurrent drought cycles. Net assimilation varied greatly at high water potentials, but as Ψ dropped below approximately -0.8 and -1.0 MPa, A was reduced to less than 1.5 μmol CO2 m-2 s-1. The relation between g and A was highly significant and conformed to an asymptotic exponential model, with A approaching maximal values at stomatal conductances of 55–65 mmol H2O m-2 s-1. Net assimilation varied linearly (r=0.95) with transpiration, and the slope of the A-E relation (WUE) was approximately 3.0 μmol CO2 mmol-1 H2O throughout the range of stomatal conductances observed. C i was insensitive to water stress, even though both g and A were strongly affected. Under the experimental conditions used here, mesophyll photosynthesis did not appear to control g through changes in C i. As stress intensified within each drying cycle, WUE of nonirrigated seedlings did not decline relative to that of controls even though CO2 and water vapor exchange rates underwent large displacements. The effect of seed source was highly significant for WUE, and the basis for observed differences among genotypes is discussed.
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  • 9
    ISSN: 1573-5079
    Keywords: chloroplast coupling factor ; induction ; thioredoxin ; regulation ; ATP synthase ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Simultaneous, non-invasive measurements were made of the rate of photosynthetic CO2 fixation and the state of activation of the chloroplast CF1CF0-ATP synthase (CF) in field-grown sunflower (Helianthus annuus L.) during the dark-to-light transition at sunrise. CO2 fixation showed a linear response with light intensity from zero to about 500–700 μE m-2 s-1. However, at light intensities of only 5–22 μE m-2 s-1, the energetic threshold for activation of the CF was found to be significantly lowered (as compared to the pre-dawn state), presumably through reduction of the regulatory sulfhdryl groups of the γ-subunit of the CF. When these studies were extended to chamber-grown plants, it was found that as little as 5 seconds of illumination at 4 μE m-2 s-1 caused apparently full CF reduction. It is clear, therefore, that the catalytic activation of CF is not rate limiting to the induction of carbon assimilation under field conditions during a natural dark-to-light transition at sunrise. A model, based on the redox properties of the regulatory sulfhydryls, was developed to examine the significance of sulfhydryl midpoint potential in explaining the differences in light sensitivity and oxidation and reduction kinetics, between the CF and other thioredoxin-modulated chloroplast enzymes. Computer simulations of the light-induced regulation of three representative thioredoxin-modulated enzymes are presented.
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  • 10
    ISSN: 1573-5079
    Keywords: chemiosmotic theory ; chloroplasts ; photosynthesis ; proton transport ; quantam requirement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Initial and steady state rates of proton transport at low light intensity have been measured and compared with steady state rates of electron transport in the pH range of 6.0–7.6 in envelope-free spinach chloroplasts. At pH 6–7, the H+/e- values computed using the initial rate of proton transport varied with light intensity, from a value of 2 at low light to almost 5 at high light. In contrast, the H+/e- values computed using the steady state rate of proton transport did not show a dependence on light intensity, having a constant value of 1.7±0.2. Likewise, at pH 7.6, the H+/e- ratio, computed using either the initial or steady state rates of proton transport did not vary with light intensity but was constant at H+/e-=1.7±0.1. Analysis of the light dependence of electron and proton transport allowed determination of (a) the quantam requirements of transport, (b) the rates of transport at light saturation, and (c) H+/e- ratios for initial and steady state proton transport. Extrapolating the initial proton transport to zero light, we found that both H+/photon and H+/e- values were not strongly dependent on pH, approaching a near constant value of 2.0. Using the initial rate of proton transport extrapolated to saturating light intensity we found the H+/e- ratio to be strongly pH-dependent. We suggest that internal pH controls electron transport at high light intensities. The true stoichiometry is reflected only in measurements taken at low light using the initial proton transport data. Our findings and interpretation reconcile some conflicting data in the literature regarding the pH-dependence of the H+/e- ratio and support the concept that internal pH controls noncyclic electron transport.
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