ALBERT

Notes: The main objective of this study is to analyse the strategic and organizational evolution of large firms in a current time period and within a specific geographical context (Spain). In order to achieve this purpose, the paper combines the Chandlerian programme with processual analysis. We draw on documentary sources (annual reports, company histories, business directories and so on) and interviews. We have found that in the model of corporate development some characteristics of new organizational structures will coexist with some features of old ones. In line with previous studies our findings highlight the existence of the ‘multidivisional network’ but, most importantly, our findings also reflect the emergence of a new kind of organizational form that we call ‘holding network’. The ‘holding network’ is more decentralized, both strategically and operationally, than the multidivisional form. However, its level of decentralization is less than in the N-form. Furthermore, whereas the multidivisional form is a hierarchy, the ‘holding network’ emphasizes the communication among people of different levels. This multilevel communication differs from that in the N-form. Apart from the horizontal communication in the same level there is vertical and horizontal communication between different levels. We conclude by emphasizing the need to explore in future empirical studies to what extent these patterns and new organizational forms should be considered as a transitory or consolidated phenomenon.

Notes: A histopathological survey of wild and cultured shrimp from the Gulf of Mexico was carried out to determine the prevalence and severity of infectious diseases and parasites, affecting four species of penaeid shrimp (Litopenaeus setiferus, Farfantepenaeus aztecus, F. duorarum, and L. vannamei). Around 60 shrimp were obtained from each of 10 sampling stations during different months in 1999 and 2000. One station was a shrimp culture farm from Tamaulipas State, and nine were wild stations from Tamaulipas (five), Veracruz (one), and Campeche (three) States. Thirty shrimp from each station were used for histological analysis. The remaining shrimp were frozen and kept at −20 C to carry out PCR techniques to determine the presence of white spot syndrome virus (WSSV). Some in situ hybridization analyses were applied to ascertain cases of WSS viral disease. Fresh analysis was carried out in shrimp from only one station for parasite identification. The histopathological survey performed on 688 shrimp showed no evidence of viral diseases. Two shrimp from one station were histologically diagnosed as having presumptive WSSV. Tissues from the same shrimp were processed for in situ hybridization and another 20 samples from the same station were processed to run PCR techniques. In each case, the results were negative. The same PCR procedure was applied to the rest of the frozen samples and none of them showed the presence of the WSSV. No important bacterial infections were observed in any of the wild or cultured shrimp analyzed. Fresh analysis demonstrated the presence of the cestode Prochristianella hispida and the gregarines Cephalolobus penaeus and Nematopsis penaeus. Histological analysis showed that the abundance of P. hispida varied from 7 to 90%, and G1 to G4 grades of severity were observed, with G1 being the most common. Gregarines were commonly observed in the histological slides, with a prevalence varying from 3 to 56% and G1 severity in most cases. Haplosporidan infection was observed in one sample of F. duorarum, which is the first report of this parasite in this host. Two invasive epibionts, the filamentous bacteria Leucothrix sp. and the ciliate Ascophrys sp., were found in four sampled stations. The study showed that in ten stations sampled from 1999 to 2000 in the Gulf of Mexico, shrimp populations had no serious infectious diseases.

Notes: The apoplastic fluid of pine (Pinus pinaster Aiton) hypocotyls contains ascorbic acid (AA) and dehydroascorbic acid (DHA). The amounts of ascorbic and dehydroascorbic acids were in the nmol (g fresh weight)−1 range and decreased with the hypocotyl age as well as along the hypocotyl axis. The ratio AA/(AA+DHA) also decreased with the hypocotyl age and along the hypocotyl. Both ascorbic oxidase and peroxidase activity against ascorbic acid showed very low activity not only in the apoplastic fluid but also in the fractions ionically and covalently bound to the cell walls. However, the peroxidase activity in the three abovementioned fractions was strongly increased in the presence of ferulic acid. That stimulation effect increased with the hypocotyl age and from the apical towards the basal region of the hypocotyls of 10-day-old seedlings. Furthermore, the oxidation of ferulic acid by apoplastic and ionically- and covalently-bound peroxidases was inhibited by ascorbic acid as long as ascorbate was available. A regulatory role of apoplastic ascorbic acid levels in the formation of dehydrodiferulic bridges between wall polysaccharides catalysed by cell wall peroxidases and thus in the cell wall stiffening during plant growth is proposed.

Notes: Phosphorus-deficient Gracilaria tenuistipitata Zhang et Xia was cultured for 15 days at two different inorganic phosphate (Pi) concentrations: 3 μM (low Pi treatmenl) or 30 μM phosphate (high Pi treatment). The amount of ribulose-l,5-bisphosphate carboxy-lase/oxygenase (Rubisco), phycobiliproteins, Chl a and total soluble proteins were higher in the high Pi than in the low Pi treatment. The total N content of the low Pi plants was lower than in plants grown at high Pi concentrations whereas the amount of total C was highest in low Pi plants. The increase of Rubisco content in the high Pi treatment (3-fold) was parallel to the enhancement of the maximum photosynthetic rate which increased 5-fold. This correspondence was also found in the low Pi treatment in which Rubisco content and maximum photosynthesis did not change significantly from the initial values. The photosynthetic efficiency was also higher at high than at low Pi. The high Pi plants also showed higher dark respiration and growth rates. The data presented here suggest that marine macroalgae submitted to Pi deficiency exhibit a decrease in growth caused not only by Pi implication on energy transfer in photosynthesis and respiration, but also by the diminution of the amount of photosynthetic pigments and Rubisco.

Notes: The influence of nitrogen on ribulose-1.5-bisphosphate carboxylase/oxygenase (Rubisco. EC 4.1.1.39) content is poorly understood in macroalgae. N-deficient Gracilaria tenuistipitata Zhang et Xia var. liui was cultivated in the laboratory under constant light intensity and temperature. Biochemical and physiological variables were monitored after a high (1 mM) or low (o. 1 mM) nitrate pulse. Rubisco content in crude extracts was estimated by SDS-PAGE with the Coomassie Blue Staining procedure. Nitrate was consumed immediately in the low-N treatment, but there was always an external nitrate source in the high-N treatment. Total soluble proteins and phycobiliproteins decreased as internal nitrogen diminished in the low-N treatment, but kept fairly constant in N-sufficient conditions. However, Rubisco content increased until the 7th day and then started to decrease in both cases. Fresh weight increment showed a better correlation with Rubisco than with pigment content.

Notes: The rates of phosphate uptake and photosynthesis were simultaneously determined in order to investigate the relationship between phosphate use and photosynthesis. Porphyra umbilicalis (L.) Kützing exhibited two different phosphate uptake kinetics. The first one followed a saturation model and was observed in light (maximum phosphate uptake rate. Vmax= 94 ± 30 nmol P1 m−2 s−1: semisaturation constant. S15= 4.0 ± 3.4 μM: phosphate compensation point. PCP = 0.3 ± 0.4 μMγ: the seeraid one was linear and worked at high external phosphate concentrations in the dark. Inhibition of photosynthesis by removing the inorganic carbon from the medium produced the same effect aa darkness on phosphate uptake. Successive bicarbonate additions produced increments of photosynthesis rate and the recovering of the phosphate uptake pattern observed in light. The results showed that Porphyra umbilicalis, at the typical phosphate concentrations in its natural habitat, takes up phosphate in the light through the operation of a photosynthetically controlled active system.