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  • Springer Nature  (209)
  • Wiley  (141)
  • Molecular Diversity Preservation International  (48)
  • Blackwell Publishing Ltd  (37)
  • 1
    Electronic Resource
    Electronic Resource
    Analysis of Antigens from Membrane and Soluble Fractions of Entamoeba histolytica (1986)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 33 (1986), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Axenic cultures of Entamoeba histolytica strains HK-9, HM-1, and Rahman were fractionated to provide plasma membranes, internal, vesiculated membranes, and a soluble cytosol. Each particulate fraction was solubilized and all fractions were analyzed by techniques designed to demonstrate molecular complexity and serologic reactivity. The cytosol contained more antigenic moieties than either membrane fraction; however, the antigens associated with the membranes had very high reactivity and lower nonspecific activity than the cytosol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1986.tb05619.x
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  • 2
    Electronic Resource
    Electronic Resource
    Isoenzyme Analysis of Babesia microti Infections in Humans (1986)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 33 (1986), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . We used high-resolution polyacrylamide gradient gel electrophoresis (PGGE) to separate four babesial enzymes to aid in the identification of two Babesia microti isolates established in hamsters. The isolates were compared to two different hamster passages of the “Gray” strain. All isoenzyme patterns from the two isolates and the “Gray” strain were similar except glucose phosphate isomerase (GPI) from one of the “Gray” strain passages. It showed a polymorphic GPI pattern as opposed to a monomorphic GPI pattern seen in the other “Gray” strain passage and the two isolates. The observed differences suggested (i) that some populations of B. microti are capable of having polymorphic GPI, (ii) that the “Gray” strain originally contained (and may still contain) a heterogenous population of B. microti, and (iii) that the population possessing polymorphic GPI was selected over that with monomorphic GPI. This information was obtained by a PGGE method that eliminated hemoglobin from gels and allowed, for the first time, detection of babesial leucine amino peptidase (LAP) and isocitrate dehydrogenase (IDH). In addition, this method provided molecular weight estimations on babesial GPI, LAP, IDH, and glutamate dehydrogenase (GDH), and it proved useful in the identification and characterization of the B. microti isolates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1986.tb05592.x
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  • 3
    Electronic Resource
    Electronic Resource
    Isoenzyme Comparison of Axenic Giardia lamblia Strains (1992)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 39 (1992), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We obtained isoenzyme patterns by polyacrylamide gradient gel electrophoresis (PGGE) of water-soluble protein fractions prepared from trophozoites of 11 axenic G. lamblia strains. The strains were isolated from animals and humans (both symptomatic and asymptomatic) from various geographic locations. Isoenzymes were also separated by isoelectric focusing. Of 12 enzymes attempted, eight exhibited well-defined and reproducible isoenzyme patterns by PGGE, based on which the strains were grouped into four zymodemes. Although the 11 strains were grouped into four zymodemes based on PGGE, no correlation between zymodeme and the known characteristics of the strains existed. Thus, a high degree of characteristic sharing appears to occur among genetically different G. lamblia strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1992.tb04851.x
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  • 4
    Electronic Resource
    Electronic Resource
    High-Resolution Polyacrylamide Gradient Gel Electrophoresis (PGGE) of Isoenzymes from Five Naegleria Species (1988)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 35 (1988), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . High-resolution polyacrylamide gradient gel electrophoresis (PGGE) was used to separate isoenzymes of 12 Naegleria strains: one N. australiensis, two N. lovaniensis, one N. jadini, two N. gruberi isolated from environmental samples, and six N. fowleri strains isolated from patients with primary amoebic meningoencephalitis. Of the eight enzymes studied, seven showed zymograms with interspecific variation that identified all the species tested. Although the six N. fowleri strains were biochemically the most homogeneous, they showed intraspecific isoenzyme variation that allowed them to be grouped into four zymodemes. The PGGE technique, which separates isoenzymes by their molecular shape, is both sensitive and economical. It offers an addition or an attractive alternative to isoelectric focusing which has commonly been used to aid species identification of Naegleria by separating isoenzymes by their isoelectric point.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1988.tb04070.x
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  • 5
    Electronic Resource
    Electronic Resource
    A Fast Electrophoretic Isoenzyme Technique for the Identification of Invasive and Non-Invasive Entamoeba histolytica and “E. hitolytica-like” Organisms (1987)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 34 (1987), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cellulose acetate electrophoresis (CAE) was used to separate glucosephosphate isomerase, hexokinase, malic enzyme, and phosphoglucomutase extracted from invasive and non-invasive Entamoeba histolytica and “E. histolytica-like” organisms. Each of these morphologically similar organisms possessed a unique CAE isoenzyme profile that can be used as an aid in their identification. The CAE technique used to obtain these isoenzyme profiles is rapid, simple, and economical, and it requires neither specialized training nor claborate equipment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1987.tb03169.x
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  • 6
    Electronic Resource
    Electronic Resource
    Limestone Influences on Physical and Chemical Features of a Mountain Stream (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 24 (1986), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Influences of limestone on physical and chemical characteristics of Abrams Creek in Cades Cove, Great Smoky Mountains National Park, were investigated from February to July 1977. Infiltration of stream water into subsurface permeable limestone conduits caused reduced and intermittent surface flow within the middle reach of the Cove. At the downstream (west) edge of the Cove, inflowing waters characteristically warmed the stream in winter and cooled it in summer and increased stream conductivity, total hardness, and pH. Management activities in the Cove did not appear to appreciably affect the chemistry of the aquatic system downstream relative to the large effects of limestone substrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1986.tb00992.x
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  • 7
    Electronic Resource
    Electronic Resource
    THE PHYSIOLOGY OF SECRETION. (1898)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 11 (1898), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1898.tb54973.x
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  • 8
    Electronic Resource
    Electronic Resource
    The Australian Register: Current Research and Future Role (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 541 (1988), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1988.tb22237.x
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  • 9
    Electronic Resource
    Electronic Resource
    Some observations on the ecology and the population dynamics of Merluccius angustimanus in the South Gulf ofCalifornia (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 7 (1975), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A population of Merluccius angustimanus Garman has been discovered in the south-east Gulf of California, where it is found at 80–120 m in the south of its range (Mazatlan to Altata) and down to 500 m at the northern end of its range (Isla San Pedro Nolasco, north of Guaymas). Studies of the otoliths and catch curves of M. angustimanus show that it lives up to seven years, and may reach 39 cm long (one fish) although it does not usually exceed 32 cm. Using the notation of Beverton & Holt (1959) the following parameters were estimated: L∞= 32.7 cm, K= 0.35, t∞= 0.016, t∞= 7years, Lm= 22.0 cm, M for 3–7 years of age = 0.79, M for 2–3 years of age = 0.79-0.89. The von Bertalanffy equation for M. angustimanus was:〈displayedItem type="mathematics" xml:id="mu1" numbered="no"〉〈mediaResource alt="image" href="urn:x-wiley:00221112:JFB83:JFB_83_mu1"/〉 and was shown to give a reasonably close fit to the real growth curve.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1975.tb04578.x
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  • 10
    Electronic Resource
    Electronic Resource
    Some observations on the ecology of Galeus piperatus Springer and Wagner, a little known shark endemic to the Northern Gulf of California (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 7 (1975), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Galeus piperatus Springer & Wagner, the peppered shark, is a dwarf shark found in the Gulf of California north of the City of Guaymas, Sonora. It has been taken in June and August 1971 and in February and March 1972, and is found from 130–550 m with maximum abundances from 170–420 m deep. Numbers are higher in June and August (up to 110 individuals/ha) than in February and March. In June and August 1971 the area of distribution included all the waters north of Isla Tiburón and Isla Angel de la Guarda, and below the 130 m isobath. During these months Galeus piperatus was found at 12 and 33 stations respectively, but was taken at only one station in the same area during February and March 1972. The fishing effort expended during these periods was 16, 42 and 60 units respectively. It seems likely that a strong migratory movement causes these differences in abundance and distribution. Galeus piperatus has been taken from 50–100 m off the bottom in mid-water trawls at night and is clearly a mid-water and demersal form. Sexually mature females carrying olive green egg cases were taken in large numbers in June 1971 and were also present in reduced numbers in August 1971. Only females 18 cm or more in total length carried egg cases. The migration of Galeus piperatus into the North Gulf of California appears to coincide with sexual maturity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1975.tb04577.x
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