ALBERT

Description: Discharge in mountain streams may be a mixture of snowmelt, water from surface runoff, and deep return flow through valley bottom alluvia. We used δ 18 O and δ 2 H, solute concentrations, and 222 Rn to determine water sources of a headwater stream located at the McDonald Creek watershed, Glacier National Park, USA during summer recession flow period. We analyzed minimal water isotope ranges of -17.6 to -16.5‰ and -133 to -121‰ for δ 18 O and δ 2 H, respectively, potentially due to dominance of snow-derived water in the stream. Likewise, solute concentrations measured in the stream through the watershed showed minimal variation with little indication of sub-surface water input into the stream. However, we observed 222 Rn activities in the stream that ranged from 39 to 2,646 Bq/m 3 with the highest value measured in middle of the watershed associated with channel constriction corresponding to changes in local orientation of underlying rocks. Downstream from this point, 222 Rn activity decreased from 581 to 117 Bq/m 3 in a series of punctuated steps associated with small rapids and waterfalls that we hypothesized to cause radon degassing with a maximum predicted loss of 427 Bq/m 3 along a 400 m distance. Based on mass balance calculations using 222 Rn activity values, streamflow, and channel characteristics, we estimated that groundwater contributed between 0.3 and 29% of total flow. Overall, we estimated a 5.9% of groundwater contribution integrated for stream reach measured at McDonald Creek during recession flow period. Finally, a lower mean hyporheic flux of 14 m 3 /day was estimated compared to the groundwater flux of 70,710 m 3 /day. These assessments highlight the potential for radon as a conservative tracer that can be used to estimate sub-surface water contribution in mountain streams within a complex geologic setting. This article is protected by copyright. All rights reserved.

Description: [1]  A single scoop of the Rocknest aeolian deposit was sieved (〈 150 µm), and four separate sample portions, each with a mass of ~50 mg, were delivered to individual cups inside the Sample Analysis at Mars (SAM) instrument by the Mars Science Laboratory rover's sample acquisition system. The samples were analyzed separately by the SAM pyrolysis evolved gas and gas chromatograph mass spectrometer analysis modes. Several chlorinated hydrocarbons including chloromethane, dichloromethane, trichloromethane, a chloromethylpropene, and chlorobenzene were identified by SAM above background levels with abundances of ~0.01 to 2.3 nmol. The evolution of the chloromethanes observed during pyrolysis is coincident with the increase in O 2 released from the Rocknest sample and the decomposition of a product of N -methyl- N -( tert -butyldimethylsilyl)-trifluoroacetamide (MTBSTFA), a chemical whose vapors were released from a derivatization cup inside SAM. The best candidate for the oxychlorine compounds in Rocknest is a hydrated calcium perchlorate (Ca(ClO 4 ) 2 ·nH 2 O), based on the temperature release of O 2 that correlates with the release of the chlorinated hydrocarbons measured by SAM, although other chlorine-bearing phases are being considered. Laboratory analog experiments suggest that the reaction of Martian chlorine from perchlorate decomposition with terrestrial organic carbon from MTBSTFA during pyrolysis can explain the presence of three chloromethanes and a chloromethylpropene detected by SAM. Chlorobenzene may be attributed to reactions of Martian chlorine released during pyrolysis with terrestrial benzene or toluene derived from 2,6-diphenylphenylene oxide (Tenax) on the SAM hydrocarbon trap. At this time we do not have definitive evidence to support a nonterrestrial carbon source for these chlorinated hydrocarbons, nor do we exclude the possibility that future SAM analyses will reveal the presence of organic compounds native to the Martian regolith.

Description: Genes in the sex determination pathway are important regulators of sexually dimorphic animal traits, including the elaborate and exaggerated male ornaments and weapons of sexual selection. In this study, we id...

Description: This paper discusses the evidence for the role of CREB in neural stem/progenitor cell (NSPC) function and oncogenesis and how these functions may be important for the development and growth of brain tumours. The cyclic-AMP response element binding (CREB) protein has many roles in neurons, ranging from neuronal survival to higher order brain functions such as memory and drug addiction behaviours. Recent studies have revealed that CREB also has a role in NSPC survival, differentiation and proliferation. Recent work has shown that over-expression of CREB in transgenic animals can impart oncogenic properties on cells in various tissues and that aberrant CREB expression is associated with tumours in patients. It is the central position of CREB, downstream of key developmental and growth signalling pathways, which give CREB the ability to influence a spectrum of cell activities, such as cell survival, growth and differentiation in both normal and cancer cells. There is significant experimental data implicating the CREB signalling pathway in the development and maintenance of brain tumours, and this knowledge will provide an opportunity to investigate novel drug targeting approaches in glioma treatment.

Description: This paper discusses the evidence for the role of CREB in neural stem/progenitor cell (NSPC) function and oncogenesis and how these functions may be important for the development and growth of brain tumours. The cyclic-AMP response element binding (CREB) protein has many roles in neurons, ranging from neuronal survival to higher order brain functions such as memory and drug addiction behaviours. Recent studies have revealed that CREB also has a role in NSPC survival, differentiation and proliferation. Recent work has shown that over-expression of CREB in transgenic animals can impart oncogenic properties on cells in various tissues and that aberrant CREB expression is associated with tumours in patients. It is the central position of CREB, downstream of key developmental and growth signalling pathways, which give CREB the ability to influence a spectrum of cell activities, such as cell survival, growth and differentiation in both normal and cancer cells.

Description: Background: The Sepsidae family of flies is a model for investigating how sexual selection shapes courtship and sexual dimorphism in a comparative framework. However, like many non-model systems, there are few molecular resources available. Large-scale sequencing and assembly have not been performed in any sepsid, and the lack of a closely related genome makes investigation of gene expression challenging. Our goal was to develop an automated pipeline for de novo transcriptome assembly, and to use that pipeline to assemble and analyze the transcriptome of the sepsid Themira biloba. Results: Our bioinformatics pipeline uses cloud computing services to assemble and analyze the transcriptome with off-site data management, processing, and backup. It uses a multiple k-mer length approach combined with a second meta-assembly to extend transcripts and recover more bases of transcript sequences than standard single k-mer assembly. We used 454 sequencing to generate 1.48 million reads from cDNA generated from embryo, larva, and pupae of T. biloba and assembled a transcriptome consisting of 24,495 contigs. Annotation identified 16,705 transcripts, including those involved in embryogenesis and limb patterning. We assembled transcriptomes from an additional three non-model organisms to demonstrate that our pipeline assembled a higher-quality transcriptome than single k-mer approaches across multiple species. Conclusions: The pipeline we have developed for assembly and analysis increases contig length, recovers unique transcripts, and assembles more base pairs than other methods through the use of a meta-assembly. The T. biloba transcriptome is a critical resource for performing large-scale RNA-Seq investigations of gene expression patterns, and is the first transcriptome sequenced in this Dipteran family.

Description: Although pedogenic barite has been documented in many modern soils and palaeosols, no actualistic studies on its formation have been reported. Because barite is stable over the entire range of pressure and temperature of the Earth's crust, it preserves reliable data about the original environment in which it formed. Pedogenic barite and barite-bearing soils have been used as indicators of landscape stability, environmental conditions, climate and microbial activity. This study compares field data, micromorphology and stable isotope geochemistry of a barite-bearing palaeosol from the Morrison Formation (Jurassic) and a modern analogue soil in south-central Texas, USA. Morrison barite-bearing palaeosols are over-thickened cumulic palaeosols that developed in subaerially exposed lacustrine sediments during an extended lake contraction event. Lateral facies relationships document changes in hydrology and duration of episaturated conditions (perched water table above the Btg horizons) that correspond to differences in barite nodule morphology and abundance. Barite precipitation occurred at a redox boundary higher on the landscape after organic matter was completely oxidized. Sulphur isotope data indicate that the initial source of sulphur was soil organic matter. Meteoric water is the likely source of oxygen for the sulphate. Barium sourced from weathering feldspars and clays. The modern analogue displays similar catenary relationships, redox features and micromorphological characteristics compared to the Morrison palaeosols, suggesting that similar pedogenic processes led to barite precipitation. Synthesized data suggest that conditions favourable to barite-bearing soil formation are low-gradient basins that have received feldspar-rich sediments (i.e. volcanically influenced basins), soils that developed near salt domes, soils that developed in exposed wetland or lacustrine sediments and coastal plain deposits. When studied in a well-documented palaeogeographic context, barite-bearing soils are valuable to palaeoclimate, palaeoenvironmental and palaeohydrological studies. Combined with regional interfluve palaeosols, barite-bearing palaeosols may document temporal changes in drainage, surface stability, and accommodation consistent with sequence boundaries/maximum flooding surfaces and climate changes. This article is protected by copyright. All rights reserved.

Description: Sexually-selected exaggerated traits tend to be unusually reliable signals of individual condition, as their expression tends to be more sensitive to nutritional history and physiological circumstance than that of other phenotypes. As such, these traits are the foundation for many models of sexual selection and animal communication, such as “handicap” and “good genes” models. Exactly how expression of these traits is linked to the bearer's condition has been a central yet unresolved question, in part because the underlying physiological mechanisms regulating their development have remained largely unknown. Recent discoveries across animals as diverse as deer, beetles, and flies now implicate the widely conserved insulin-like signaling pathway, as a common physiological mechanism regulating condition-sensitive structures with extreme growth. This raises the exciting possibility that one highly conserved pathway may underlie the evolution of trait exaggeration in a multitude of sexually-selected signal traits across the animal kingdom. From the elaborate tails of peacocks to the enlarged head-horns of dung beetles, sexually-selected exaggerated traits are conspicuous and reliable signals of individual condition. How is the reliability and honesty of the signal maintained? We propose that co-option of the ancient and highly conserved insulin/insulin-like signaling pathway is the key.

Description: Fluvially derived tuffaceous Chinle sandstones from Petrified Forest National Park provide a well-preserved Late Triassic archive of climate information. Petrographic analysis of 38 Chinle sandstones provides new insight into the depositional history and evolution of palaeoclimate during Chinle deposition. This study focuses on the relationship between climate and meteoric diagenesis as a guide for constraining climate change in western equatorial Pangea during the Late Triassic. Petrographic analysis of Chinle sandstones reveals their wide range of textural attributes, as well as pedogenic and shallow burial diagenetic features that occurred during the Late Triassic. These diverse petrological characteristics are indicative of the evolving Late Triassic climate, when placed into a well-constrained stratigraphic and geochronological framework. The stratigraphic succession is characterized by variations in the abundance of framework grains, detrital matrix, weathering intensity of feldspar and volcanic rock fragments, and the mineralogy of clay cements. Climate records from Chinle palaeosol geochemistry indicate a progression from wet to dry conditions. This trend is also reflected in the meteoric diagenetic features of Chinle sandstones. During deposition of the lower Chinle, elevated rainfall promoted the weathering of labile volcanic detritus to kaolinite whereas feldspars (especially plagioclase) were partially or completely dissolved. In the upper Chinle, a trend towards dryer conditions favoured the formation of smectite and less feldspar dissolution resulting in a higher abundance of well-preserved plagioclase grains. Shallow burial meteoric weathering reactions in Chinle sandstones reflect the evolving climate during the Late Triassic. This article is protected by copyright. All rights reserved.

Description: Increased apposition of the frontal and parietal bones of the skull during embryogenesis may be a risk factor for the subsequent development of premature skull fusion, or craniosynostosis. Human craniosynostos...