Digitale Medien
Palo Alto, Calif.
:
Annual Reviews
Annual Review of Pharmacology
46 (2006), S. 215-234
ISSN:
0362-1642
Quelle:
Annual Reviews Electronic Back Volume Collection 1932-2001ff
Thema:
Medizin
,
Chemie und Pharmazie
Notizen:
New methods to measure thiol oxidation show that redox compartmentation functions as a mechanism for specificity in redox signaling and oxidative stress. Redox Western analysis and redox-sensitive green fluorescent proteins provide means to quantify thiol/disulfide redox changes in specific subcellular compartments. Analyses using these techniques show that the relative redox states from most reducing to most oxidizing are mitochondria 〉 nuclei 〉 cytoplasm 〉 endoplasmic reticulum 〉 extracellular space. Mitochondrial thiols are an important target of oxidant-induced apoptosis and necrosis and are especially vulnerable to oxidation because of the relatively alkaline pH. Maintenance of a relatively reduced nuclear redox state is critical for transcription factor binding in transcriptional activation in response to oxidative stress. The new methods are applicable to a broad range of experimental systems and their use will provide improved understanding of the pharmacologic and toxicologic actions of drugs and toxicants.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1146/annurev.pharmtox.46.120604.141122
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