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Ouabain binding in rectal gland ofSqualus acanthias (1983)

Silva, Patricio ; Epstein, Jonathan A. ; Stevens, Arthur ; [et al.]

Springer

The journal of membrane biology 75 (1983), S. 105-114

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ISSN: 1432-1424

Keywords: shark rectal gland ; ouabain binding ; (Na, K)-ATPase ; cyclic AMP ; slices ; isolated cells

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Summary In an attempt to examine the mechanisms of activation of (Na, K)-ATPase when epithelial transport is stimulated, the binding of ouabain to rectal gland tissue was measured before and after stimulation with dibutyryl cAMP and theophylline. Stimulation significantly altered the characteristics of ouabain binding to slices ofSqualus acanthias rectal gland and to isolated rectal gland cells, accelerating the rate of binding and increasing the amount of ouabain bound at equilibrium when low concentrations of ouabain (10−9 to 10−7 m) were present in the medium. Scatchard plots of ouabain binding were nonlinear, suggesting at least two classes of binding sites, one of higher and one of lower affinity. Stimulation with cAMP and theophylline appeared to increase the affinity of the high-affinity site. Ouabain binding was increased by cAMP and theophylline even in the presence of furosemide (10−4 m) or bumetanide (10−5 m), and when Li+ was substituted for Na+, or NO 3 − for Cl−-maneuvers known to inhibit rectal gland secretion. The changes in ouabain binding induced by cAMP and theophylline do not appear, therefore, to be secondary to secretory activity but may reflect a change in the configuration, environment or location of existing enzyme so as to enhance its activity. Stimulation of ouabain binding cannot be demonstrated in whole homogenates of rectal gland, indicating that intact cells are necessary for the cyclic AMP-induced increase in ouabain binding to become manifest.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01995630

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Cyclic AMP-dependent stimulation of Na,K-ATPase in shark rectal gland (1986)

Marver, D. ; Lear, S. ; Marver, L. T. ; [et al.]

Springer

The journal of membrane biology 94 (1986), S. 205-215

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ISSN: 1432-1424

Keywords: Na,K-ATPase ; vanadate ; rubidium ; shark rectal gland ; ouabain ; cAMP ; theophylline ; furosemide

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Summary Scatchard analysis of3H ouabain bound to isolated rectal gland cells as a function of increasing ouabain concentrations produced a concave curvilinear plot that was resolved into two specific sites with either a high (I) or low (II) affinity for ouabain. Cyclic cAMP/theophylline (±furosemide, 10−4 m) increased the amount of3H ouabain bound to the high-affinity site I. Vanadate, a phosphate congener which promotes formation of the ouabain-binding state of the enzyme, mimicked the effects of cAMP/theophylline at low concentrations of ouabain, suggesting that cAMP/theophylline increases binding to site I by enhancing the rate of turnover of resident enzyme. Enhanced86Rb uptake seen following cAMP/theophylline administration was primarily associated with increased flux through the high-affinity ouabain site, and this stimulation was not obliterated by the co-administration of furosemide. A model was presented which suggested the presence of two noninteracting pools of enzyme or isozymes which exhibit either a high or low affinity for ouabain. Cyclic AMP both stimulated turnover via site I, and modified the kinetics of binding of3H ouabain to site II. The (ave)K d of3H ouabain for site II was increased from 3.6 μm (controls) to 0.5 μm (cAMP/theophylline) and the Hill coefficient was modified from 0.45 (controls) to 1.12 (caMP/theophylline), suggesting a transition from a negative- to a noncooperative binding state. While furosemide reversed the effects of cAMP/theophylline on site II kinetics, it did not obliterate cAMP/theophylline effects on site I. This suggests that cAMP may alter the intrinsic turnover rate of this particular pool of Na,K-ATPase in shark rectal gland.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01869716

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Neotropical ant gardens II. Bioassays of seed compounds (1990)

Davidson, D. W. ; Seidel, J. L. ; Epstein, W. W.

Springer

Journal of chemical ecology 16 (1990), S. 2993-3013

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ISSN: 1573-1561

Keywords: Ant ; ant-garden ; benzothiazole ; Camponotus ; Hymenoptera ; Formicidae ; chemical mimicry ; epiphyte ; 2-hydroxy-3-methoxybenzene-methanol ; 1-(2-hydroxy-4-methoxyphenyl)ethanone ; 2-hydroxy-6-methyl-benzoic acid ; methyl ester ; 1-(2-hydroxy-6-methylphenyl)ethanone ; 1-(2,4-dihydroxyphenyl)ethanone ; limonene ; 6-methyl-methylsalicylate ; 6-MMS ; seed dispersal ; symbiosis ; tropical forests ; vanillin

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Abstract A number of volatile compounds occur on the seeds of taxonomically unrelated ant-garden epiphytes in western Amazonia. In field trials in southeastern Peru, we assayed the responses of ant-garden ants (Camponotus femoratus) to these and structurally similar compounds applied to artificial “seeds” made from zeolite molecular sieves. Benzothiazole,2, present on seeds of eight ant-garden epiphytes, repelled ants over the range of concentrations tested, as did 1-(2-hydroxy-6-methylphenyl)ethanone,3, occurring on seeds of six ant-garden epiphytes. 2-Hydroxy-6-methylbenzoic acid, methyl ester (methyl-6-methylsalicylate, 6-MMS),1, found on seeds of at least nine ant-garden epiphytes, was mildly repellent at high concentration, but stimulated excitement, seed handling, and (rarely) seed carrying at lower concentrations. Vanillin,5, a seed compound of four ant-garden epiphytes, and limonene,6, a monoterpene from seeds of three ant-garden epiphytes, both stimulated excitement, alarm, seed handling, and (rarely) seed carrying. Identified from seeds of seven ant-garden epiphytes, 1-(2,4-dihydroxyphenyl)ethanone,4, elicited little or no response. Among 70 compounds tested (mainly aromatic compounds), those found on seeds of ant-garden epiphytes or having structural features in common with such compounds were the most attractive to the ants. Although not present on epiphyte seeds, 2-hydroxy-3-methoxybenzenemethanol,10, consistently stimulated seed transport to the nest in one year, but did so only rarely in subsequent years. Some of the volatile compounds on seeds of ant-garden epiphytes probably play a role in ant attraction to epiphyte seeds, but evidence remains ambiguous. Finally,Ca. femoratus responded to one test compound [1-(2-hydroxy-4-methoxyphenyl)ethanone,60] (absent from epiphyte seeds) by descending from the vegetation to the ground.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00979490

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Neotropical ant gardens (1990)

Seidel, J. L. ; Epstein, W. W. ; Davidson, D. W.

Springer

Journal of chemical ecology 16 (1990), S. 1791-1816

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ISSN: 1573-1561

Keywords: Ant ; ant garden ; Hymenoptera ; Formicidae ; Camponotus ; chemical mimicry ; citronellol ; convergent evolution ; epiphyte ; mellein ; methyl 6-methylsalicylate ; Perú ; seed dispersal ; symbiosis ; tropical forest

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Abstract In ant gardens of lowland Amazonia, parabiotic ant speciesCamponotus femoratus andCrematogaster cf.limata parabiotica cultivate a taxonomically diverse group of epiphytic plants, whose establishment is restricted to arboreal carton ant nests. Epiphyte seeds are collected by workers ofCa. femoratus, the larger of the two ants, and stored unharmed in brood chambers where they subsequently germinate. Although seeds of some ant-garden epiphytes bear nutritional rewards, previous studies have shown that these rewards are not sufficient to explain the pattern of ant attraction to seeds. Five aromatic compounds occur frequently in and on the seeds of most ant-garden epiphytes and may be chemical cues by which ants recognize propagules of their symbiotic plants. The most widely distributed of these is methyl 6-methylsalicylate [6-MMS]1, previously reported as a major mandibular gland product in relatedCamponotus species and present in trace quantities inCa. femoratus males. (−)-Citronellol6 (previously unreported inCamponotus) was the principal volatile constituent in extracts of male heads, and (−)-mellein7 was present in small quantities. Discovery of 6-MMS inside the mandibular glands of maleCa. femoratus (and its presence in analogous glands of related ants) offers preliminary support for Ule's (1906) hypothesis that seeds attract ants by mimicking ant brood. In addition, the likely fungistatic activity of seed compounds suggests that they could retard microbial pathogens of ants and plants in the organic detritus of nest gardens. While the presence of identical seed compounds in so many unrelated plant lineages might represent a remarkable case of convergent evolution, other interpretations are possible.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01020495

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