Publication Date:
2004-11-16
Description:
Although androgen ablative therapy can suppress growth of localized prostate cancer (PC) cells that are androgen receptor positive, skeletal metastases and tumor relapse ultimately dictate PC related morbidity and mortality. Bone metastasis of PC cells results from a complex cascade of events that involves PC cell intravasation into blood or lymphatic vessels, followed by recruitment by bone marrow microvascular endothelial cells and trans-endothelial migration (TEM) in the bone marrow microenvironment. We have tested the hypothesis that enhanced expression of the chemokine receptor, CXCR4 in PC cells plays a crucial role in their adhesion to the endothelium followed by TEM into the bone microenvironment. To test this hypothesis, PC3 (bone metastatic PC cell line) and LnCaP (lymph node metastatic PC cell line) cells were treated with stromal derived factor-1 alpha (SDF-1α, 100 ng/ml) for 6 or 24 hr and RT-PCR amplification of both CXCR4 and GAPDH mRNAs was carried out. Within 6 hr CXCR4 levels of mRNA expression were increased (3–4 fold) in PC3 cells but not in LnCaP cells. By 24 hr the CXCR4 mRNA levels in PC3 cells returned back to normal and remain unchanged in LnCaP cells. The treatment of PC3 cells with SDF-1α (100 ng/ml) for 6 hr showed a significant (p
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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