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  • Articles  (27)
  • Springer  (19)
  • American Society of Hematology  (4)
  • Springer Nature  (3)
  • MDPI  (1)
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  • Articles  (27)
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  • 1
    Electronic Resource
    Electronic Resource
    Metachromatic leukodystrophy: a 12-bp deletion in exon 2 of the arylsulfatase A gene in a late infantile variant (1995)
    Springer
    Human genetics 〈Berlin〉 96 (1995), S. 357-360 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sequencing of the arylsulfatase A gene in a late infantile metachromatic leukodystrophy patient showed the presence of a 12-bp deletion in exon 2. This deletion was found in a compound heterozygous state with the previously described 287 C→T transition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210424
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  • 2
    Electronic Resource
    Electronic Resource
    Stable expression of the GUS reporter gene in chrysanthemum depends on binary plasmid T-DNA (1994)
    Springer
    Plant cell reports 14 (1994), S. 59-64 
    Details
    ISSN: 1432-203X
    Keywords: Agrobacterium ; transformation ; T-DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three chrysanthemum (Dendranthema grandiflora) cultivars were cocultivated with 2 Agrobacterium tumefaciens strains in combination with 4 pBIN19 derived binary plasmids, all carrying the Nosnptll selection gene and 35Sgus(intron) reporter gene. All binary plasmids transferred DNA to chrysanthemum explants but only pMOG410 gave good stable expression of GUS. This plasmid differs from the other plasmids in 2 aspects: 1) It carries a restored nptll gene and 2) the selection gene is positioned at the left border side of the reporter gene. Cocultivation with AGLO(pMOG410) yielded up to 13 GUS positive shoots per 100 explants. The presence of the gus and nptll gene in recovered shoots was confirmed by PCR and Southern blot analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233300
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic transformation of Chrysanthemum using wild type Agrobacterium strains; strain and cultivar specificity (1991)
    Springer
    Plant cell reports 9 (1991), S. 505-508 
    Details
    ISSN: 1432-203X
    Keywords: Agrobacterium ; Tumour induction ; Dendranthema grandiflora ; Polymerase chain reaction ; ß-Glucuronidase ; Neomycine phosphotransferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few genotype/strain combinations resulted in significant tumour formation. Especially 0-type strains were highly efficient. An 0-type strain was used to transfer genes for neomycine phosphotransferase (NPT II) and ß-glucuronidase (GUS) to a susceptible cultivar. Transfer of the GUS gene was confirmed by using the Polymerase Chain Reaction (PCR).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232106
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  • 4
    Electronic Resource
    Electronic Resource
    Relation between energy metabolism, glycolysis, noradrenaline release and duration of ischemia (1996)
    Springer
    Molecular and cellular biochemistry 160-161 (1996), S. 187-194 
    Details
    ISSN: 1573-4919
    Keywords: ATP breakdown ; catecholamine ; glycogen ; ischemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We studied the effect of 12–36 min of global ischemia followed by 36 min of reperfusion in Langendorff perfused rabbit hearts (n = 26). Metabolism was determined in terms of peak and total release of purines (adenosine, inosine, hypoxanthine), lactate and noradrenaline during reperfusion; and myocardial content of nucleotides (ATP, ADP, AMP), glycogen and noradrenaline at the end of reperfusion. An inverse relationship (r = −0.79) existed between duration of ischemia and developed pressure post-ischemia. Early during reperfusion, after 12 min of ischemia, the purine concentration (peak release) increased 100x (p 〈 0.01), that of lactate and noradrenaline lOx (p 〈 0.05) . Total purine release rose with progression of the ischemic period (30x after 36 min of ischemia; p 〈 0.01), concomitant with a reduction in nucleotide content. Lactate release was independent from the duration of ischemia, although glycogen had declined by 30% (p 〈 0.01) after 36 min of ischemia. The acid insoluble glycogen fraction, which presumably contains proglycogen, increased substantially during short-term ischemia. Peak noradrenaline increased 100x and 200x (p 〈 0.05) after 24 and 36 min of ischemia, respectively. Total noradrenaline release due to various periods of ischemia mirrored its peak release. Function recovery was inversely related to total purine and noradrenaline efflux (both r =−0.81); it correlated with tissue nucleotide content (r = 0.84). In conclusion, larger amounts of noradrenaline are released only after a substantial drop in myocardial ATP. During severe ischemia ATP consumption more than limited ATP production by anaerobic glycolysis, is a key factor affecting recovery on subsequent reperfusion. In contrast to lactate efflux, purine and noradrenaline release are useful markers of ischemic and reperfusion damage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00240049
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  • 5
    Electronic Resource
    Electronic Resource
    Abstracts of papers Pharmacological Meeting (1993)
    Springer
    Pharmacy world & science 15 (1993), S. I1 
    Details
    ISSN: 1573-739X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01875421
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  • 6
    Electronic Resource
    Electronic Resource
    Evaluation of screening techniques and determination of criteria for assessing resistance to Corynebacterium michiganense in tomato (1976)
    Springer
    Euphytica 25 (1976), S. 405-414 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; screening techniques ; Corynebacterium michiganense ; assessing resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary When screening populations segregating for resistance to Corynebacterium michiganense a rapid and reliable inoculation technique is needed. From the several techniques tested, inoculation of the petiole was found to give better differentiation of resistant and susceptible plants than did inoculation of the root or leaf. When petiole-inoculation was compared with stem-inoculation the latter showed the lowest environmental variation and is suggested for use in genetic studies. Stem-inoculation involved clipping the stems of 4 week old seedlings 1 cm above the cotyledonary leaves with scissors which had been dipped in inoculum. Petiole inoculation and, to a lesser degree, stem inoculation failed to produce uniform infection, resulting in variation in the expression of the disease. The scoring was therefore based on the presence, and not the degree, of wilting and stunting, or the size of the cankers. Plants free of all these symptoms were rated resistant. The coefficient of variation for vascular discoloration on individual plants was calculated to be 50%, which in part may be explained by the histopathology of the host. The virulent isolates cm 21 caused death of seedlings resistant to isolate H. However, cultivars resistant to isolate H in the seedling stage did not decrease in fruit weight as compared to susceptible cultivars when inoculated with isolate cm 21 at anthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041573
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  • 7
    Electronic Resource
    Electronic Resource
    Genetic analysis of flower and lateral shoot production in Gerbera (1985)
    Springer
    Euphytica 34 (1985), S. 785-791 
    Details
    ISSN: 1573-5060
    Keywords: Gerbera jamesonii ; gerbera ; cut flower yield ; lateral shoots ; inheritance ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An incomplete diallel cross with selfs and reciprocals was made with twelve cultivars of Gerbera jamesonii. Very significant differences occurred between GCA's of the parents for cut flower yield, earliness and number of lateral shoots. The selfs were mainly responsible for the significant SCA's. A positive genetic correlation occurs between the number of lateral shoots at anthesis of the first flower and total flower production. The phenotypic performance of parents (measured on cuttings) was poorly related to their breeding value (measured on seedlings). It is suggested that this is due to physiological differences between cuttings and seedlings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035417
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  • 8
    Electronic Resource
    Electronic Resource
    Genetic analysis of cut flower longevity in Gerbera (1985)
    Springer
    Euphytica 34 (1985), S. 779-784 
    Details
    ISSN: 1573-5060
    Keywords: Gerbera jamesonii ; gerbera ; cut flower longevity ; vase life ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The vase life duration and stem stiffness of 12 parent clones and 77 progenies of Gerbera jamesonii were studied both in summer and in winter. In winter, vase life was shorter and the stems were weak. Few exceptions from this general pattern were detected. The average vase life duration ranged from 16.0 days for the best to 8.9 days for the worst progeny. Individual plants were selected with a vase life of 20 or more days. The curvature of the stem, measured after 24 hrs dry storage, is associated with the folding of the stem in the vase. Selection for low curvature may decrease the incidence of folding and with it the variation in vase life of Gerbera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035416
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  • 9
    Electronic Resource
    Electronic Resource
    Life history studies of the leafminer Liriomyza trifolii on susceptible and resistant cultivars of Dendranthema grandiflora (1991)
    Springer
    Euphytica 56 (1991), S. 47-53 
    Details
    ISSN: 1573-5060
    Keywords: Chrysanthemum morifolium ; Dendranthema grandiflora ; host plant resistance ; leafminer ; life history ; Liriomyza trifolii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The feeding, fecundity, mortality and rate of development of Liriomyza trifolii (Burgess) on 12 cultivars of Dendranthema grandiflora (Tzvel.) (Syn. Chrysanthemum morifolium (Ramat.)) were determined in ‘no choice’ experiments. Number of feeding punctures, number of eggs laid and total number of larvae varied between cultivars but these characters were not strongly associated with leafminer population increase. Good indicators of host plant resistance were larval mortality, number of pupae and duration of the larval period. Of these three, larval mortality which could be as high as 98% was most suitable. Based on life history data it was predicted that Liriomyza trifolii cannot survive on the resistant cultivar Penny Lane. This prediction was verified experimentally.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041743
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  • 10
    Electronic Resource
    Electronic Resource
    Restoring adventitious shoot formation on chrysanthemum leaf explants following cocultivation with Agrobacterium tumefaciens (1993)
    Springer
    Plant cell, tissue and organ culture 32 (1993), S. 263-270 
    Details
    ISSN: 1573-5044
    Keywords: Dendranthema grandiflora ; preculture ; regeneration ; transformation ; β-glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Explants from leaves of in vitro-grown chrysanthemum (Dendranthema grandiflora Tzvel.) cultivars regenerated adventitious shoots without an intermediate callus phase. Puncturing explants with a brush increased regenerations, but in combination with cocultivation with Agrobacterium tumefaciens it had an adverse effect on shoot formation. The negative effect of brushing and cocultivation could be overcome by preculturing explants for 8 days. Preculture altered the location of transformed sites but did not inhibit transformation. Regeneration following cocultivation with Agrobacterium is also encouraged if alternative regeneration protocols are used that do not require brushing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042287
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