ISSN:
1572-8900
Keywords:
Transgalactosidation
;
galactosidase
;
oligosaccharides
;
guar
;
enzymatic hydrolysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Energy, Environment Protection, Nuclear Power Engineering
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract Two types of enzymatic reactions are given here as examples of synthetic problems encountered in industry. In the first case, commercially available β-D-galactosidase from Escherichia coli was used as a catalyst to transfer galactose from β-lactose to oligosaccharides. A preference for galactosyl transfer to the 3- or 4-position of the sugar moiety of the oligosaccharide was observed for the products. As expected, only the β-anomer was produced. A wide variety of sugars, including disaccharides, trisaccharides, cellotetraose, and maltodextrins, has been shown to act as acceptors, yielding oligosaccharides. In the second example, α-galactomannan that had been previously treated to contain cationic groups (cationic guar gum) was subjected to treatment with a series of inexpensive commercial enzymes such as lipases, protease, and cellulases. Some enzyme preparations showed significant changes in the viscosities of 1% cationic guar solution. For example, lipases from Aspergillus niger and Aspergillus saitoi and protease XIII from Rhizopus niveus produced a substantial viscosity reduction (0–20% of original viscosity). These examples demonstrate the utility of low-cost enzymes in manipulating polymer structures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1021833917049
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