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  • 1
    Electronic Resource
    Electronic Resource
    Indirect spectrophotometric determination of cyanide (1989)
    Springer
    Microchimica acta 98 (1989), S. 259-265 
    Details
    ISSN: 1436-5073
    Keywords: cyanide ; spectrophotometry ; indirect determination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A spectrophotometric method for cyanide based on its inhibition of the colour formation reaction between nickel(II) and 3-(4′,5′-dimethyl-2′-thiazolylazo)-2,6-dihydroxybenzoic acid has been developed, and allows the determination of down to 0.1 μg of cyanide. Most of the interferences can be avoided by displacement of the hydrogen cyanide using an arsine generator.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01244602
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  • 2
    Electronic Resource
    Electronic Resource
    4th order derivative spectrophotometric determination of quinine in soft drinks (1993)
    Springer
    Microchimica acta 110 (1993), S. 263-268 
    Details
    ISSN: 1436-5073
    Keywords: quinine ; derivative spectrophotometry ; soft drinks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A method is developed for the 4rd order derivative spectrophotometric determination of down to 1–6 μg/ml of quinine. This method has been applied to its determination in soft drinks. Comparison are made between this method and a spectrofluorimetric method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01245110
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  • 3
    Electronic Resource
    Electronic Resource
    Simultaneous determination of cobalt and chromium by ion chromatography with chemiluminescence detection and its application to glass analysis (1992)
    Springer
    Microchimica acta 106 (1992), S. 227-234 
    Details
    ISSN: 1436-5073
    Keywords: glass analysis ; cobalt determination ; chromium determination ; ion chromatography ; chemiluminescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A method is described for the simultaneous determination of very low levels of Co and Cr by high performance ion chromatography coupled with a chemiluminescence detection system. 0.1M K2SO4 solutions, adjusted to pH 3.0, were used as eluent to separate Co(II) and Cr(III) between them and from interferents. The detection system was the chemiluminescence of luminol/ H2O2 in alkaline medium catalyzed by such transition metals. Using a matrix solution analogous to soda-lime silica glass (dissolved in acids) calibration graphs were linear up to 0.5 ng ml−1 for cobalt and up to 250 ng ml−1 for chromium. The corresponding calculated detection limits (3 s) in such matrix were 0.05 ng ml−1 and 15 ng ml−1 for Co and Cr, respectively. The relative standard deviations were 1.4% at 0.5 ng ml−1 Co level and 2.8% at the 200 ng ml−1 Cr level. No interferences were observed from the more common metals, particularly those present in glass samples. The ion chromatography/ chemiluminescent method proposed has been successfully applied to the analysis of Co and Cr in glasses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01242094
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular genetic analysis of patients carrying steroid 21-hydroxylase deficiency in the Mexican population: identification of possible new mutations and high prevalence of apparent germ-line mutations (1998)
    Springer
    Human genetics 〈Berlin〉 102 (1998), S. 170-177 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Steroid 21-hydroxylase deficiency is the underlying cause in over 90% of patients with congenital adrenal hyperplasia, an inherited metabolic disorder of adrenal steroidogenesis. We have characterized 94 mutant alleles from 47 unrelated Mexican patients and the corresponding mutant alleles in their parents by amplification of the functional CYP21 gene by PCR, followed by direct sequence analysis. The study included patients diagnosed with the three clinical forms of the disease. Our results revealed: (1) the presence of relatively few mutations or combinations of mutations associated with particular phenotypes; (2) the presence of putative new mutations; (3) the finding of identical genotypes in patients displaying discordant phenotypes; (4) the identification of patients lacking all previous reported mutations; and (5) an apparent high frequency of germ-line mutations. The absence of previously reported mutations in about 22% of the disease alleles, the finding of putative new mutations in some of the patients lacking previously known mutations, and the apparent high prevalence of germ-line mutations make evident the differences in the genetic background leading to this disorder between the Caucasian and the Mexican populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050672
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  • 5
    Electronic Resource
    Electronic Resource
    Characterization and DNA sequence of the b6w2 allotype of the rabbit immunoglobulin kappa 1 light chain (b locus) (1995)
    Springer
    Immunogenetics 42 (1995), S. 333-341 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The b6w2 allotype of the constant region of the rabbit immunoglobulin kappa 1 (k1) light chain (b locus) was discovered in wild populations from northern Spain. At the serological level, the b6w2 allotype is characterized by the presentation of all b6-specific epitopes, while an allotypic determinant which is shared between the nominal b5 and b6 allotypes is lacking. The DNA fragment encoding the b6w2 allotype was amplified by means of the polymerase chain reaction, and sequenced directly by dideoxy-DNA-sequencing. When compared with the sequence of the nominal b6 allele, the b6w2 sequence differs at eleven nucleotide positions (96.5% similarity). This variation corresponds to amino acid replacements at 1) the three positions C-terminal to the peptidyl junction with the variable region (amino acid positions 109–111);2) the four positions N-terminal to the interdomain disulfide bond (167–170); and 3) two positions in the vicinity of the interchain disulfide bond (190 and 210). The nature and distribution of the observed nucleotide substitutions strongly suggest a possible role of the extra interdomain disulfide bond in the unusual evolutionary dynamics of the rabbit K1 light chain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00179394
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  • 6
    Electronic Resource
    Electronic Resource
    The allotypic patchwork pattern of the rabbit IGKC1 allele b5wf: genic exchange or common ancestry? (1999)
    Springer
    Immunogenetics 49 (1999), S. 7-14 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The protein sequences of different alleles of the rabbit immunoglobulin IGKC1 gene can differ at more than 40% of the amino acid positions. This exceptional degree of allelic divergence raises questions concerning the causal underlying mechanisms. We report the DNA sequence of the coding region of an allotype which is associated with the mitochondrial lineage A (Southwestern Spain). At the serological level, this b5wf allotype presents a patchwork of antigenic determinants which in domestic breeds are characteristic of the b4, b5, and b6 allotypes. The inferred protein sequence of the b5wf allotype was found to differ from that of the b4, b5, and b6 allotypes at 25, 10, and 15% of the amino acid positions, respectively. Sequence comparisons show that the b4-specific epitopes of the b5wf allotype are probably due to a shared ThrThrGlnThr motif at Kabat positions 153–156. Similarly, the shared b5-specific determinants should relate to the motifs 161ThrSerLys163 and/or 182LysSerAspGlu185. A monoclonal antibody binding epitope shared among the b5wf, b5, and b6 sequences appeared to be correlated with the presence of Asp190. Although there is evidence of interallelic genic exchange, sequence comparisons suggest that the apparent mosaic structure of the b5wf allotype is better explained by common ancestry and point mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050458
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  • 7
    Electronic Resource
    Electronic Resource
    Effect of inorganic carbon supply on the photosynthetic physiology of Gracilaria tenuistipitata (1994)
    Springer
    Planta 194 (1994), S. 55-61 
    Details
    ISSN: 1432-2048
    Keywords: Carbonic anhydrase ; Chlorophyll ; Gracilaria ; Inorganic carbon ; Ribulose-1,5-bisphosphate carboxylase/oxygenase ; Phycobiliprotein ; Photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gracilaria tenuistipitata Zhang et Xia was cultured for 15 d at low, normal and high inorganic carbon concentrations under constant light, temperature and nutrient conditons. Carbonic anhydrase (CA; EC 4.2.1.1.) activity, ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco; EC 4.1.1.39) content, pigment content and C/N ratio were measured, and also the photosynthesis and growth rates. Both Rubisco content and CA activity increased under conditions of low inorganic carbon (Ci) but decreased at high Ci with respect to the control. The amount of pigments declined considerably at high Ci and was slightly higher at low Ci. The maximum rate of photosynthesis and the photosynthetic efficiency increased in low Ci and the opposite was found at high Ci concentration. The effects of Ci concentration on maximum rate of photosynthesis and photosynthetic efficiency are discussed in relation to the variation in pigment and Rubisco contents and CA activity. The data indicate that Ci may be an important factor controlling the photosynthetic physiology of G. tenuistipitata with regard, not only to the enzymes of Ci metabolism, but also to the pigment content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201034
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  • 8
    Electronic Resource
    Electronic Resource
    Modulation of Synapsin I Gene Expression in Rat Cortical Neurons by Extracellular Matrix (1998)
    Springer
    Cellular and molecular neurobiology 18 (1998), S. 369-378 
    Details
    ISSN: 1573-6830
    Keywords: neuronal differentiation ; synapsin I ; extracellular matrix ; laminin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. Neuronal differentiation depends on crosstalk between genetic program and environmental cues. In this study we tried to dissect this complex interplay by culturing neurons from fetal rat brain cortices in a chemically defined, neuron-specific, medium and on different substrata, either artificial (poly-D-lysine) or natural. 2. Among the extracellular matrix compounds used in this study, two (collagen I and fibronectin) allowed only a weak attachment of cortical neurons to the substratum, while the others (collagen IV, laminin, and basal lamina from Engelbreth-Holm-Swarm sarcoma) allowed both firm attachment and moderate to extensive neurite outgrowth from neuronal cell bodies. 3. By using synapsin I gene expression as a parameter of neuronal differentiation, we found that neurite outgrowth and neuronal differentiation are not linearly linked. Synapsin I gene expression, in fact, was maximal in neurons cultured on laminin, while the fastest neuritic outgrowth was recorded in cultures on poly-D-lysine. 4. The data presented in this paper are consistent with the hypothesis that the extracellular matrix plays an active role in modulating the differentiative program of neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022562813846
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  • 9
    Electronic Resource
    Electronic Resource
    Isabela Crater-Lake: a Mexican insular saline lake (1998)
    Springer
    Hydrobiologia 381 (1998), S. 1-7 
    Details
    ISSN: 1573-5117
    Keywords: Tropical limnology ; meromixis ; nutrients ; plankton ; Nayarit ; Mexico ; Isabela island
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Isabela Crater-Lake is a bright-green, hypersaline lake (68–112.5 mS cm-1) on Isabela Island off the Pacific coast of Nayarit, Mexico. Some salient features were documented in November 1993. It appears meromictic, with three well-defined strata separated by sharp pycnoclines. Surface water was warm (32 °C) reaching a subsurface (0.5–1 m) maximum temperature (33 °C), declining gradually to 26.7 °C at maximum depth (17.5 m). Dissolved oxygen was near saturation at the surface, attained 145 percent saturation at 0.5 m, but was completely absent by 2.5 m. Eh was maximum at the surface (123 mV), declining to a minimum at 3 m (–261 mV), and was about –240 mV from 3.5 m to the bottom. The pH varied from 9.3 in surface waters to slightly acid (6.4) in deep anoxic layers. Atypically, NO3 was more abundant than NH4 in both aerobic and anaerobic strata. PO4 and SiO2 concentrations were extremely high. The planktonic microbial community was formed by four groups: bacteria (photosynthetic sulfur bacteria and heterotrophic bacteria), phytoflagellates (mainly cryptomonads), heterotrophic nanoflagellates ( Spumella spp., Bodo spp.) and ciliates (Hypotricha and Oligotricha). Bacteria occurred throughout the water column, but other biota were restricted to surface waters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003239910574
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  • 10
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    dc four-point resistance of a double-barrier quantum pump (2009)
    American Physical Society
    Details
    Publication Date: 2009-02-27
    Print ISSN: 1098-0121
    Electronic ISSN: 1550-235X
    Topics: Physics
    Published by American Physical Society
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