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  • Springer  (173)
  • American Physical Society  (26)
  • 1
    ISSN: 1573-4943
    Keywords: cyclic AMP derivatives ; affinity labels ; chemical modification of enzymes ; nucleotide affinity labels ; cAMP phosphodiesterase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The syntheses of two potential cAMP affinity lables, 1,N 6-(3-iodopropyleno)adenosine 3′,5′-cyclic-monophosphate and 2′-O-(2-iodo-3-hydroxypropyl) adenosine 3′,5′-cyclic-monophosphate, by a two-step chemical procedure are described. TheN 6- and 2′-O-allyl intermediates were prepared selectively by alkylation of cAMP in organic and alkaline aqueous solutions, respectively. Treatment of theN 6-allyl derivative withN-iodosuccinimide resulted in iodine addition to the double bond and cyclization to theN 1 position of the purine ring. The iodohydrin analog was synthesized by reaction of 2′-O-allyl-cAMP with potassium iodide and thallium trichloride in acetate buffered solution. The products were isolated by column chromatography and characterized by thin-layer chromatography, elemental analysis, and ultraviolet,13C, and1H NMR spectroscopy. The cAMP analogs were found to react with lysine and cysteine. Both cAMP derivatives were tested for their reaction with the low-K m cAMP phosphodiesterase of human platelets. The ribose-substituted analog functioned as a competitive inhibitor (K I =0.72 μM) and caused a time-dependent irreversible inactivation of the phosphodiesterase. In contrast, the purine-substituted derivative acted neither as a reversible competitive inhibitor nor as an irreversible inactivator of the enzyme. These results indicate the specificity of these potential cAMP analogs in their interaction with the phosphodiesterase.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of mathematical biology 42 (1980), S. 861-870 
    ISSN: 1522-9602
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Mathematics
    Notes: Abstract A mathematical model of prothrombin activation is being proposed which includes the feedback mechanism of thrombin and the alteration of factor V by thrombin. This model is in good agreement with experimental data for the dependence of the rate of thrombin formation on the concentrations of factors V and X a . In particular, it correctly predicts the existence and location of a maximum in both of these cases.
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  • 3
    ISSN: 1522-9602
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Mathematics
    Notes: Abstract In the study of chemical modification of enzymes and other biologically active proteins, plots of fractional residual activity as a function of number of groups modified per enzyme molecule are often used to establish a correlation between the chemical modification and enzyme inactivation reactions and to determine the stoichiometry of the modification reaction. This paper presents a critical examination of the underlying theoretical framework of such graphs. Whereas these plots are usually presented as linear functions, it is shown here that the general equation describing the relationship between inactivation and modification contains an exponential term; therefore, in the general case, the plot is actually a curve. It is suggested that caution be exercised in the interpretation of such plots and that equations such as those derived in the text be used to fit theoretical curves to the data, in order to maximize the information gained from chemical modification experiments.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 28 (1972), S. 706-707 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Resumen Los nucleos de las células grasas de larvas deDrosophila melanogaster, contienen cromosomas politénicos cuya ultrastructura es similar a la que presentan los cromosomas en las glandulas salivales. Bandas gruesas, finas, y algunas «minibandas» se hallan separadas por regiones claras compuestas por fibrillas con un diametro de 60–80 Å. El nucleolo constituido por elementos granulares periféricos y fibrilares centrales, presenta un aspecto similar al nucleolo de glandulas salivales.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 33 (1977), S. 301-302 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The protein kinase binding assay for cAMP was modified by substitution of adsorption by QAE cellulose for the membrane filtration. This modification obviates the variation of recovery of cAMP with the volume of buffer used to wash the filter. The assay is reproducible and technically simpler than those currently employed.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 25 (1969), S. 1306-1307 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Resumen La tinción con plata y con fucsina básica del nucleolo en células meristemáticas deAllium cepa permite detectar la localización de dos componentes de distinta afinidad tintorial. El componente de mayor argentofilia aparece en forma de gránulos, distribuídos en el interior de una matriz contínua que presenta marcada basofilia.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Mineralogy and petrology 51 (1994), S. 355-375 
    ISSN: 1438-1168
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Description / Table of Contents: Zusammenfassung Die Skiddaw-Gruppe im Liegenden des mittelordovizischen Vulkanbogens des englischen Lake District enthält kleinere Intrusionen von kalkalkalischen, HornblendeAugit-reichen, dioritischen bis pikritischen Magmen, die an das Appinit-Vorkommen in den schottischen Kaledoniden erinnern. Für diese Intrusionen ist der Scawgill Bridge-Mikrodiorit repräsentativ. Er stellt einen variablen, Hornblende-reichen Körper mit Einschlüssen von Augit-porphyrischem Meladiorit und Pyroxenit dar; zu den anderen gehören die “pikritischen” (Augit-Meladiorit oder Cortlandit) Stöcke bei Dash und Barkbeth. Sie unterscheiden sich von tholeiitischen Intrusionen gleichen Alters, die durch den Embleton-Diorit vertreten sind, und auch von den spätsilurischen Biotit-Lamprophyren. Trotz Alteration werden primäre Korrelationen, die durch immobile Spurenelemente angezeigt werden, auch von Hauptelementen einschließlich Mg, Fe und Ca nachgezeichnet. Die Differentiation bei Scawgill Bridge wird als eine Fraktionierung von Olivin, Augit, Hornblende und Chromit modelliert, und ein ähnlicher Prozeß wird für die Meladiorit-Intrusion angenommen. Daraus wird geschlossen, daß diese Körper primitive, kalkalkalische Magmen repräsentieren, die zur Unteren Borrowdale Volcanic Group gehören und die unter hohem PH2O fraktionierten. Im Gegensatz dazu wird die Untere Borrowdale Volcanic Group selbst von plagiophyrischen Gesteinen dominiert, die durch Fraktionierung unter niedrigem P(inH2O) entstanden sind.
    Notes: Summary Within the Skiddaw Group, which underlies the mid-Ordovician volcanic arc of the English Lake District, are minor intrusions of calc-alkaline, hornblende-augite rich, dioritic to picritic bodies reminiscent of the appinite suite of the Scottish Caledonides. Representative of these is the Scawgill Bridge microdiorite, a variable, hornblendic body which includes augite-phyric meladiorite and pyroxenite; others include the ‘picrtte’ (augite-meladiorite or cortlandtite) plugs at Dash and Barkbeth. They are distinct from tholeiitic intrusions of similar age represented by the Embleton Diorite, and also from late Silurian biotite-lamprophyres. Despite alteration, primary variation trends indicated by immobile trace elements are also expressed by major elements including Mg, Fe and Ca. Differentiation at Scawgill Bridge is modelled as fractionation of olivine, augite, hornblende and chromite, and a similar process is inferred for the meladiorite intrusions. It is concluded these bodies represent primitive, calc-alkaline magmas related to the Lower Borrowdale Volcanic Group, which fractionated under confined conditions of high P(inH2o). In contrast, the Lower Borrowdale Volcanic Group itself is dominated by plagiophyric rocks which resulted from fractionation at lower P(inH2O).
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 62 (1975), S. 439-440 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe a portable, non-motorized device for delivering a tracer dye into seawater under field conditions. Dye is ejected at a constant flow rate over a period of tens of minutes. The ejector works in a wide range of ambient pressures without external energy requirements. The flow rate is adjusted simply by varying the length of the delivery tube. The dye streams permitted observations of the upcurrent and downcurrent flow regimes for a filter-feeding crinoid (Comanthus bennetti) living at a depth of 8 m on a coral reef. The results indicate that the crinoid may enhance the rate of particle capture by changing the scale of turbulence in the water passing through the mesh of the filtration fan.
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  • 10
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shell growth in Yoldia eightsi was measured over an austral summer and winter in 1992. In specimens 〈 12 mm length, growth was not significantly different between summer and winter periods, and the fastest recorded rate, 6.3 μm day−1 was for 5-mm individuals during the winter. In summer, specimens of all lengths grew significantly, but in winter bivalves 〉 27 mm length did not increase in length. Tissue dry and ash-free dry mass (AFDM) cycles were assessed at monthly intervals between December 1988 and January 1991. ANCOVA indicated significant interannual and seasonal effects on this cycle. Tissue mass increased in the summer, coinciding with the phytoplankton bloom and the period of maximum sedimentation of organic material from the water column. A standard 20-mm-length animal reached a maximum AFDM of 114 mg in February 1990. The minimum value (68 mg AFDM) throughout the 2 years of measurements was in early December 1988, at the end of the austral winter. Periods of tissue mass increase were, therefore, decoupled from shell growth, at least in juveniles. Tissue mass was significantly higher in 1990 than 1989, which was mainly due to high organic contents in the summer (January to May). This was not consistent with the pattern of organic content in the sediments at the study site, but was in phase with the cycle in sediment chlorophyll a content. Tissue mass increase depended on major resource input during the summer, but Y. eightsi was capable of maintaining winter condition from stocks of benthic microalgae in years of poor ice cover. Tissue mass declined between April and July each year. This was accompanied by large falls in tissue ash content, and coincided with the spawning period in early June. These are the first monthly tissue mass data collected over a 2-year period for an Antarctic mollusc. They are the first such data indicating seasonal variation in tissue mass and showing a decoupling of shell and tissue growth in a polar bivalve. The P/B ratio calculated from these data was 0.106, which is slightly lower than previous values found for this species, but is in line with general values for Antarctic marine benthos.
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