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  • Medicago (root nodules, N2 fixation)  (2)
  • 05 A 15  (1)
  • Springer  (3)
  • American Physical Society
  • Blackwell Publishing Ltd
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Publisher
  • Springer  (3)
  • American Physical Society
  • Blackwell Publishing Ltd
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  • 1
    ISSN: 1432-2048
    Keywords: Aspartate aminotransferase (immunocytochemistry, subcellular fractionation) ; Glutamate oxaloacetic transaminase ; Medicago (root nodules, N2 fixation) ; Nitrogen fixation ; Root nodule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aspartate aminotransferase (AAT; EC 2.6.1.1) catalyzes the synthesis of the amino acid aspartate which, in alfalfa root nodules, serves as the immediate precursor of the primary N-transport compound, asparagine. The enzyme AAT may also be important in providing substrates for host-plant and bacteroid respiration. The enzyme occurs as two isoenzymes, AAT-1 and AAT-2, with AAT-1 more abundant in roots and AAT-2 predominant in root nodules. To further elucidate the role of AAT in root-nodule metabolism, subcellular fractionation and immunocytochemical methods were used to determine the intra- and intercellular localization of these two isozymes. Fractionation of nodule subcellular components showed that AAT-2 was localized in amyloplasts. Immunogold labelling with AAT-2 antibodies unequivocally confirmed this, showing that AAT-2 was localized in nodule amyloplasts and leaf chloroplasts. In root nodules, the density of immunogold labelling of infected cell plastids was almost four times that of uninfected cell plastids. The data suggest that aspartate biosynthesis in alfalfa root nodules occurs primarily in the plastids of infected cells.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Combinatorica 16 (1996), S. 465-477 
    ISSN: 1439-6912
    Keywords: 05 A 15 ; 05 A 16 ; 05 A 20 ; 60 C 05 ; 41 A 10 ; 68 R
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract It is often required to find the probability of the union of givenn eventsA 1 ,...,A n . The answer is provided, of course, by the inclusion-exclusion formula: Pr(∪A i )=∑ i −∑ i〈j Pr(A i ∩A j )±.... Unfortunately, this formula has exponentially many terms, and only rarely does one manage to carry out the exact calculation. From a computational point of view, finding the probability of the union is an intractable, #P-hard problem, even in very restricted cases. This state of affairs makes it reasonable to seek approximate solutions that are computationally feasible. Attempts to find such approximate solutions have a long history starting already with Boole [1]. A recent step in this direction was taken by Linial and Nisan [4] who sought approximations to the probability of the union, given the probabilities of allj-wise intersections of the events forj=1,...k. The developed a method to approximate Pr(∪A i ), from the above data with an additive error of exp $$( - O(k/\sqrt n ))$$ . In the present article we develop an expression that can be computed in polynomial time, that, given the sums ∑|S|=j Pr(∩ i∈S A i ) forj=1,...k, approximates Pr(∪A i ) with an additive error of exp $$( - \bar \Omega (k^2 /n))$$ . This error is optimal, up to the logarithmic factor implicit in the $$\bar \Omega$$ notation. The problem of enumerating satisfying assignments of a boolean formula in DNF formF=v l m C i is an instance of the general problem that had been extensively studied [7]. HereA i is the set of assignments that satisfyC i , and Pr(∩ i∈S A i )=a S /2n where ∧ i∈S C i is satisfied bya S assignments. Judging from the general results, it is hard to expect a decent approximation ofF's number of satisfying assignments, without knowledge of the numbersa S for, say, all cardinalities $$1 \leqslant |S| \leqslant \sqrt m$$ . Quite surprisingly, already the numbersa S over |S|≤log(n+1)uniquely determine the number of satisfying assignments for F. We point out a connection between our work and the edge-reconstruction conjecture. Finally we discuss other special instances of the problem, e.g., computing permanents of 0,1 matrices, evaluating chromatic polynomials of graphs and for families of events whose VC dimension is bounded.
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  • 3
    ISSN: 1432-2048
    Keywords: Aspartate aminotransferase (immunocytochemistry, subcellular fractionation) ; Glutamate oxaloacetic transaminase ; Medicago (root nodules, N2 fixation) ; Nitrogen fixation ; Root nodule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aspartate aminotransferase (AAT; EC 2.6.1.1) catalyzes the synthesis of the amino acid aspartate which, in alfalfa root nodules, serves as the immediate precursor of the primary N-transport compound, asparagine. The enzyme AAT may also be important in providing substrates for host-plant and bacteroid respiration. The enzyme occurs as two isoenzymes, AAT-1 and AAT-2, with AAT-1 more abundant in roots and AAT-2 predominant in root nodules. To further elucidate the role of AAT in root-nodule metabolism, subcellular fractionation and immunocytochemical methods were used to determine the intra- and intercellular localization of these two isozymes. Fractionation of nodule subcellular components showed that AAT-2 was localized in amyloplasts. Immunogold labelling with AAT-2 antibodies unequivocally confirmed this, showing that AAT-2 was localized in nodule amyloplasts and leaf chloroplasts. In root nodules, the density of immunogold labelling of infected cell plastids was almost four times that of uninfected cell plastids. The data suggest that aspartate biosynthesis in alfalfa root nodules occurs primarily in the plastids of infected cells.
    Type of Medium: Electronic Resource
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