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  • Ultrastructure  (7)
  • Median eminence  (6)
  • Springer  (12)
  • American Physical Society
  • BioMed Central
  • Public Library of Science
  • Springer Nature
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 200 (1979), S. 329-334 
    ISSN: 1432-0878
    Keywords: Median eminence ; Axon terminals ; Tanycytes ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present ultrastructural study proves the existence of nerve terminals closely apposed to the plasmalemmata of tanycytes in the rat median eminence. Several of these “axo-tanycytic” endings display remarkable accumulations of agranular endoplasmic reticulum in the form of pleomorphic vesicles which are closely apposed on either side of the plasma membrane of each cell compartment. Some of these vesicular profiles give the impression of structural continuity across both membrane systems. This phenomenon is discussed in the context of being a potential substratum for communication between both cell compartments.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 162 (1975), S. 61-73 
    ISSN: 1432-0878
    Keywords: Scanning-transmission electron microscopy ; Median eminence ; Third ventricle ; Supraependymal neurons ; Supraependymal histiocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A combined scanning/transmission electron microscopic (SEM/TEM) technique was used to analyze the third cerebral ventricle and underlying tissue of the median eminence of 6 mature rhesus monkeys. The same sample of the ventricular wall was subjected to both SEM and TEM. This technique demonstrates two basic subpopulations of supraependymal cells on the surface of the supraoptic, infundibular and mammillary recesses. Type 1 cells are definitely neuron-like in their surface configuration and internal fine structural organization. Type 2 cells are more similar to histiocytes and are not as numerous as type 1 cells. The functional capacity of type 1 cells is discussed in the context of their potential role as a neuronal network that may serve as a short loop autoregulatory mechanism controlling the synthesis of releasing hormones or biogenic amines.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 138 (1973), S. 475-488 
    ISSN: 1432-0878
    Keywords: Synaptogenesis ; Spinal cord ; Chick embryo ; Coated vesicles ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptogenesis was studied in 3, 4, 5 and 8 day embryos. A small number of synapses were located in the marginal zone near the motor region in 3–4 day embryos. At 5 days the number of synapses increased and synapses were also found within the motor region. At 8 days there was a large increase in the total number of synapses and most were found within the motor region. At this stage, for the first time, many knobs contained flattened synaptic vesicles. Synapses on the perikarya were rarely but occasionally observed both at the 5 and 8 day stages. A few synapses were located in the marginal zone near dorsal root entry at the 5 day stage and the number increased by the 8 day stage. Although this sequence of synaptic development resembles that found in the monkey fetus, differences in behavioral development between these two species indicate that descriptive relationships between synaptic and behavioral development must be made cautiously. Furthermore, evidence is presented which indicates that the junctional specialization is the first sign of a developing synapse and that coated vesicles, possibly derived from the Golgi apparatus, which are fused to the neural plasmalemma may be related to the initial formation of the junctional specialization.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 175 (1976), S. 265-277 
    ISSN: 1432-0878
    Keywords: Median eminence ; Macaca mulatto ; High-voltage electron microscopy ; Neurosecretion ; Ependymal cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the normal median eminence of the male rhesus monkey (Macaca mulatta) is described using high-voltage electron microscopy. Surface specializations of ependymal cells lining the infundibular recess included cilia, apical extrusions, and microvilli. Supraependymal cells were predominantly macrophage-like, but examples of lymphocytic types were also seen. Tanycytes had long, branching, basal processes filled with numerous microtubules, some lipid droplets, and granules. The zona interna was composed of large unmyelinated neurosecretory fibers. A few myelinated fibers were also seen, but their character as neurosecretory fibers could not be established. The zona externa was composed of densely-packed profiles of neurosecretory fibers of small diameter, was well-vascularized and contained the terminations of tanycytes. Perivascular glial cells, vesiculated elements, pituicytes, and cellular elements common to connective tissue were observed. The intricate relationships between both the cellular and fibrous elements of the median eminence can be appreciated with the capability of high-voltage electron microscopy to discern ultrastructure in sections 10 times thicker than those used for low-voltage electron microscopy. The median eminence of this primate species has an ultrastructural organization similar to that described for most other species.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 105 (1970), S. 1-32 
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Deafferentation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This report concerns a light and electron microscopic investigation of the median eminence and dorsal infundibular stem of the rat following surgical isolation (deafferentation). Using a modification of the Halász technique, the basal hypothalamus, including the arcuate nucleus and median eminence were surgically isolated from surrounding structures. Special attention was directed to the contact (external) zone of the median eminence and rostral infundibulum where tuberohypophyseal axons as well as ependymal cell processes abut upon the abluminal basement membrane of the portal perivascular space. The results of this study to date suggest that 9, 20, and 40 days following surgical isolation, there is a distinct increase in the population of tuberohypophyseal dense core vesicles. It is suggested that deafferentation abolishes inhibitory and excitatory input that serves to modify the cellular dynamics of tuberohypophyseal neurosecretory elements. Comments are also made on the presence of cistern-like structures in the lateral median eminence; the presence of vesicle-like inclusions in terminal ependymal processes is discussed in relationship to the role that ependyma may play in linking the third ventricle with the adenohypophysis.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 211 (1980), S. 191-206 
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Transplants ; Vasopressin ; Median eminence ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Blocks of anterior hypothalamus were transplanted from 19 day-old fetuses of Wistar/Lewis rats into the third ventricle of adult male Brattleboro rats. Physiological changes in graft recipients and in sham-operated animals were monitored daily. Twenty days after surgery, the graft recipients and shamoperated animals were killed and their brains examined by correlative scanning and transmission electron microscopy. Host animals that exhibited both decreased polydipsia and increased urine concentration were found to have viable grafts within the third ventricle. The observed physiological changes suggested that synthesis and release of vasopressin occurred in the transplanted neurons. Grafts were well vascularized by vessels arising from the host hypothalamus. Neurons, with perikarya ranging from 8 to 30 μm in diameter, glial cells, and neurites were located throughout the transplants. A neurohemal contact zone, similar to that normally seen in the median eminence, could not be demonstrated in the grafts. The absence of complete glial and ependymal barriers indicates a relatively close association between cells in the transplants and the cerebrospinal fluid. A large increase in supraependymal neurons and their processes, including an eruption of neurons through the floor of the third ventricle in one animal, was observed in graft recipients but not in shamoperated animals.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 131 (1986), S. 1-10 
    ISSN: 1615-6102
    Keywords: Cell division ; Lomentaria ; mitosis ; Red algae ; Rhodophyta ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitosis in the marine red algaLomentaria baileyana (Rhodymeniales, Rhodophyta) was studied with the electron microscope. Nucleus associated organelles known as polar rings (PRs) migrate to establish the division poles at prophase. At prometaphase, shallow invaginations in the nuclear envelope (NE) form on two sides of each PR and soon rupture. The gaps that are consequently formed contain several small fragments of NE. A larger region of NE remains intact between the two gaps. By metaphase several cisternae of perinuclear endoplasmic reticulum (PER) have enclosed most of the nucleus but remain absent from the polar regions. The nucleolus disperses partially and a typical metaphase plate of chromosomes is formed. Each PR has disjoined into separate proximal and distal portions. MTs converge widely on all regions of the polar area, but do not extend into the cytoplasm. Some MTs end near or at the chromosomes while others extend slightly farther past the chromosomes or diagonally to the NE. As chromosomes move to opposite poles at anaphase, they are accompanied by nucleolar material. An interzonal midpiece (IZM) is created as the pole to pole distance increases and the NE remains intact except for the polar gaps. Following detachment from the IZM, the daughter nuclei are separated by a large central vacuole as a cleavage furrow develops and eventually constricts to form two cells following pit connection formation. It is suggested that mitosis inLomentaria represents an evolutionary intermediate between that seen in the higher and lower groups of red algae. This conclusion is in agreement with conventional morphological and light microscopic criteria used to placeLomentaria in theRhodymeniales, which is considered to be the next to most advanced order in theRhodophyta.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 118 (1983), S. 56-70 
    ISSN: 1615-6102
    Keywords: Carposporophyte ; Cell division ; Mitosis ; Rhodophyta ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitotic ultrastructure was observed in meristematic cells of carposporophyte generations of the freshwater red algaBatrachospermum ectocarpum. Prior to nuclear division, cell elongation occurs resulting in the nucleus being located at the proximal end of the cell and separated by a large central vacuole from a distal “empty region” which the daughter nucleus eventually occupies. In late prophase, nuclear envelope-attached polar rings are situated adjacent to shallow nuclear invaginations. At prometaphase the nuclear invaginations extend deeply into the nucleus forming continuous cytoplasmic channels containing microtubules. Perinuclear ER and a typical equatorial chromosomal plate are formed by metaphase at which time the nuclear envelope lining the cytoplasmic channels has dispersed. Chromosomal and non-chromosomal microtubules converge at the poles where a single, large gap is seen in the otherwise intact nuclear envelope. Polar rings were not detected in the few mitotic cells observed beyond prometaphase but are thought to be present. During anaphase an interzonal midpiece is formed and the distal-most incipient daughter nucleus moves laterally past the central vacuole into the apical “empty region”. Features of mitosis inBatrachospermum are believed to be intermediate between those exhibited by the lower and higher orders of red algae, this being consistent with the taxonomic placement of the genus in theNemaliales, the least advanced order of the classFlorideophyceae.
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  • 9
    ISSN: 1615-6102
    Keywords: Dasya ; Mitosis ; Rhodophyta ; Spermatangia ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitosis in the marine red algaDasya baillouviana (Ceramiales, Florideophyceae) was observed with the electron microscope. Most details of the process are quite similar to those observed in the other macroscopic red algae studied to date. However, some minor variations were noted. At late prophase a very small nuclear envelope protrusion (NEP) is formed at each division pole subjacent to the “nucleus associated organelle” known as the polar ring (PR) and 2–3 cisternae of perinuclear endoplasmic reticulum (PER) are commonly present during metaphase-anaphase. In contrast, in the other florideophycean algae where mitosis has been reported, a prominent NEP is present at late prophase (McDonald personal communication,Scott et al. 1980) and only a single cisterna of PER is observed. Additionally, during mid-late interphase and in mitotically-quiescent cells ofDasya, a single cisterna of smooth-surfaced ER is always juxtaposed with each PR. The possible significance of PER in theFlorideophyceae and other multinucleate organisms is discussed as well as the likely functions of spindle-associated smooth ER. It is suggested that ultrastructural features of mitosis should be useful as criteria to aid in the interpretation of the phylogeny of red algae.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 121 (1984), S. 199-208 
    ISSN: 1615-6102
    Keywords: Psychotria ; Leaf nodules ; Calyx nodules ; Symbiosis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The occurrence and structure of calyx nodules in the flowers of two leaf nodulated rubiaceous speciesPsychotria punctata Vatke andPsychotria kirkii Hiern. has been described for the first time at the ultrastructural level. Bacteria, resident in colleter-secreted mucilage in the space between calyx and corolla, invade stomatal pores which develop on the calyx protoderm. The bacteria proliferate in the substomatal cavity and then invade the calyx mesophyll. This invasion is most pronounced inP. punctata where the bacteria even penetrate and enter the cells of the vascular tissue. Although no sheath forms around the calyx nodules, the calyx mesophyll cells surrounded by the bacteria become identical in shape, size and secretory function to the invasive mesophyll cells of leaf nodules. The functional and evolutionary significance of calyx nodulation is discussed.
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