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  • Pollen  (5)
  • Springer  (5)
  • American Physical Society
  • BioMed Central
  • Cell Press
  • Nature Publishing Group
  • Springer Nature
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  • Springer  (5)
  • American Physical Society
  • BioMed Central
  • Cell Press
  • Nature Publishing Group
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Years
  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructure of the sperm of Plumbago zeylanica (1984)
    Springer
    Planta 162 (1984), S. 385-391 
    Details
    ISSN: 1432-2048
    Keywords: Angiosperm reproduction ; Mitochondrion (in pollen) ; Plastid (in pollen) ; Plumbago ; Pollen ; Sperm cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pollen grains of Plumbago zeylanica L. were serially sectioned and examined using transmission electron microscopy to determine the three-dimensional organization of sperm cells within the microgametophyte and the quantity of membrane-bound organelles occurring within each cell. Sperm cells occur in pairs within each pollen grain, but are dimorphic, differing in size, morphology and organelle content. The larger of the two sperm cells (Svn) is distinguished by the presence of a long (approx. 30 μm) projection, which wraps around and lies within embayments of the vegetative nucleus. This cell contains numerous mitochondria, up to two plastids and, infrequently, microbodies. It is characterized by a larger volume and surface area and contains a larger nucleus than the other sperm cell. The second sperm cell (Sua) is linked by plasmodesmata with the Svn, but is unassociated with the vegetative nucleus. It is smaller and lacks a cellular projection. The Sua contains relatively few mitochondria, but numerous (up to 46) plastids and more microbodies than the other sperm. The degree of dimorphism in their content of heritable cytoplasmic organelles must at fertilization result in nearly unidirectional transmission of sperm plastids into just one of the two female reproductive cells, and preferential transmission of sperm mitochondria into the other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393450
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitative cytology of the sperm cells of Brassica campestris and B. oleracea (1987)
    Springer
    Planta 170 (1987), S. 446-452 
    Details
    ISSN: 1432-2048
    Keywords: Brassica (sperm cell) ; Cytoplasmic inheritance ; Double fertilization ; Mitochondrion ; Pollen ; Vegetative nucleus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pollen grains of Brassica campestris L. var. acephala DC and B. oleracea L. were serially sectioned and examined using transmission electron microscopy to determine the three-dimensional organization of sperm cells within the microgametophyte and the quantity of membrane-bound organelles occurring within each cell. Sperm cells occur in pairs within each pollen grain, but are dimorphic, differing in size, morphology and mitochondrial content. The larger of the two sperm cells (Svn) is distinguished by the presence of a blunt evagination, which in B. oleracea wraps around and lies within shallow furrows on the vegetative nucleus and in B. campestris can penetrate through internal enclaves of the vegetative nucleus. This sperm cell contains more mitochondria in both species than the second sperm cell (Sua). This latter cell is linked to the first by a common cell junction with the S vn, but is not associated with the vegetative nucleus and lacks a cellular evagination. Such differences are indicative of a system of cytoplasmic heterospermy in which sperm cells possess significantly different quantities of mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402978
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  • 3
    Electronic Resource
    Electronic Resource
    Activation and developmental regulation of an Arabidopsis anther-specific promoter in microspores and pollen of Nicotiana tabacum (1993)
    Springer
    Sexual plant reproduction 6 (1993), S. 217-224 
    Details
    ISSN: 1432-2145
    Keywords: Microspore development ; Pollen ; Transcriptional regulation ; Transient expression ; Microprojectile bombardment ; β-Glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seven cytologically distinct stages during micro spore development were identified and used to define the activation of an Arabidopsis anther-specific gene (apg) in transgenic tobacco plants containing an apg promoter-gus fusion. Histochemical analysis of GUS activity showed that the apg promoter was activated in miduninucleate microspores prior to equatorial and polar nuclear migration. Quantitative analysis in isolated spores showed that GUS activity per milligram of protein decreased progressively during pollen development, but on a per spore basis showed a second peak of activity in mid-bicellular pollen. Activation of the apg promoter in isolated gametophytic cells during development was also investigated following DNA delivery by microprojectile bombardment. Levels of transient expression were detectable in uninucleate microspores, but peaked in mid-bicellular pollen, in contrast to the progressive increase in activity of the promoter of the ‘late’ pollen gene lat52. These data show that the apg promoter is activated in a biphasic pattern, and indicate that the activity of transcription factors which mediate apg promoter activity persist through microspore mitosis, but decrease during pollen maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231898
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  • 4
    Electronic Resource
    Electronic Resource
    Calcium distribution in fertile and sterile anthers of a photoperiod-sensitive genic male-sterile rice (1998)
    Springer
    Planta 204 (1998), S. 183-192 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Anther ; Calcium ; Male sterility ; Oryza (fertility) ; Pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Potassium antimonate was used to locate Ca2+ in fertile and sterile anthers of a photoperiod-sensitive genic male-sterile rice (Oryza sativa L. japonica). During the development of fertile anthers, abundant calcium precipitates accumulated in the anther walls and on the surface of pollen grains and Ubish bodies at the late developmental stage of the microspore, but not in the cytoplasm of pollen grains. Following the accumulation of starch grains in pollen, calcium precipitates on pollen walls diminished and increased in parenchymatous cells of the connective tissue. In sterile anthers, calcium precipitates were abundant in the middle layer and endothecium, but not in the tapetum, as was found in fertile anthers. A special cell wall was observed between the tapetum and middle layer of sterile anthers that appeared to relate to distinctive calcium accumulation patterns and poor pollen wall formation in the loculi. The formation of different patterns of antimonate-induced calcium precipitates in the anthers of photoperiod-sensitive genic male-sterile rice indicates that anomalies in the distribution of calcium accumulation correlate with the failure of pollen development and pollen abortion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050245
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  • 5
    Electronic Resource
    Electronic Resource
    Brassica napus pollen oleosins possess a characteristic C-terminal domain (1995)
    Springer
    Planta 195 (1995), S. 469-470 
    Details
    ISSN: 1432-2048
    Keywords: Brassica (oleosin, pollen) ; Oleosin ; Pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sequences of Brassica napus L. pollen oleosins have been determined and examined. Contrary to a recent report, inferred primary sequences of pollen oleosins do include a unique C-terminal domain characterised by the presence of a repetitive motif of three alanine and one proline residue (AAAP). This motif appears to be present in all oleosins expressed in pollen, but not in oleosins from other tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202607
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