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  • 1
    Electronic Resource
    Electronic Resource
    Levodopa effect on norepinephrine and dopamine brain levels after portocaval shunt in rats (1982)
    Springer
    Cellular and molecular life sciences 38 (1982), S. 386-387 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In various brain areas in the rat, after 7 days of portocaval shunt with levodopa administration, we found an increase in norepinephrine and dopamine levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949409
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  • 2
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    Levodopa effect on norepinephrine and dopamine brain levels after portocaval shunt in rats (1982)
    Springer
    Details
    Publication Date: 1982-03-01
    Print ISSN: 0014-4754
    Topics: Biology , Medicine
    Published by Springer
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    PAPER CURRENT
  • 3
    Electronic Resource
    Electronic Resource
    Spectrophotometric determination of vanadium with 4-(1′H-1′,2′,4′-Triazolyl-3′-azo)-2-methylresorcinol (1983)
    Springer
    Microchimica acta 80 (1983), S. 349-354 
    Details
    ISSN: 1436-5073
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Vanadin bildet bei pH 8,10 (Tris-HClO4-buffer) mit 4-(1′H-1′,2′,4′-tri-azolyl-3′-azo)-2-methylresorcin ein rosenrot-violettes Chelat, dessen Absorptionsmaximum bei 525 nm in Gegenwart von 50% Methylalkohol gemessen wird. Dieser 1∶1-Komplex entspricht bei einer Vanadin-Konzentration von 0,1–1,51μg/ml dem Beerschen Gesetz; seine molare Absorptivität ist 2,55×104l·mol−1·cm−1. Das Verfahren wurde zur Bestimmung des Vanadins in Stahl verwendet.
    Notes: Summary 4-(1′H-1′,2′,4′-Triazolyl-3′-azo)-2-methylresorcinol reacts with vanadium(V) at pH 8.10 (Tris-HClO4 buffer solution) to produce a pink-violet 1∶1 complex (λ max=525 nm,ε=2.55×104l·mol−1· cm−1) in a 50% (v/v) methanol-water medium, which allows the spectrophotometric determination of 0.1 to 1.51 ppm of vanadium. The method has been applied for the determination of the vanadium content in low alloy steels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01202011
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  • 4
    Electronic Resource
    Electronic Resource
    Changes in brain oxidative metabolism in rats with portocaval shunt (1980)
    Springer
    Cellular and molecular life sciences 36 (1980), S. 1403-1404 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Oxygen and glucose consumption in brain slices of rats were found to be diminished after 7 days of portocaval shunt. This decrease may be an index of early metabolic alterations produced by the operation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01960125
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  • 5
    Electronic Resource
    Electronic Resource
    Light- and electron-microscopical localization of calcitonin, calcitonin gene-related peptide, somatostatin and C-terminal gastrin/cholecystokinin immunoreactivities in rat thyroid (1989)
    Springer
    Histochemistry and cell biology 91 (1989), S. 265-272 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Parafollicular C cells of the rat thyroid contain several immunoreactive peptides including calcitonin (CT), calcitonin gene-related peptide (CGRP), somatostatin and a C-terminal gastrin/CCK immunoreactive epitope as shown at the light-and electron-microscopical levels. Adult thyroid C cells are strongly immunoreactive to CT and most of the cells also react strongly with CGRP antisera and weakly with a gastrin/CCK antiserum. The latter antiserum may cross-react with CGRP. This cross-reactivity probably only occurs at very high concentrations of CGRP observed in adult thyroid C cells, but not in intrathyroidal CGRP-containing nerves, nor in early neonatal C cells. In neonatal rats, somatostatin immunoreactive C cells are numerous and most of these cells are also CT and CGRP immunoreactive. In contrast, only few C cells display somatostatin immunoreactivity in adult rat thyroids. Sequential staining experiments revealed that some thyroidal C cells simultaneously express all four types of immunoreactivity. At the electron microscopical level, all of these immunoreactivities were observed in secretory granules of C cells. Double- and triple-staining experiments, moreover, documented that some peptides are co-localized in the same granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493000
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  • 6
    Electronic Resource
    Electronic Resource
    Simultaneous determination of drugs in concentration ratios above 40 : 1 by application of multivariate calibration to absorbance and derivative spectrophotometric signals (1997)
    Springer
    Fresenius' journal of analytical chemistry 357 (1997), S. 449-456 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The performance of two multivariate calibration methods, multiple linear regression (MULTI3) and partial least-square regression (PLS-2), for the resolution of the ternary mixtures of acetylsalicylic acid-caffeine-codeine and acetaminophen-caffeine-codeine is compared. The methodologies were checked by applying them to the analysis of two sets of laboratory-prepared mixtures over the concentration ranges 13.0–19.0, 1.00–3.00 and 0.20–1.00 μg · ml–1 for acetylsalicylic acid-caffeine-codeine and 12.0–22.0, 0.40–2.00 and 0.20–1.00 μg · ml–1 for acetamimophen-caffeine-codeine, respectively. While the results provided by MULTI3 were unacceptable in the majority of the cases, those obtained by PLS-2 were quite good with considerably diminished errors, as a result of calibration and/or checking with MULTI3, taking no account of potential interactions between analytes. It was shown that it is possible by using PLS-2 to resolve complex mixtures of analytes in a highly disparate proportion, even when they have overlapping signals. The proposed method was successfully demonstrated for pharmaceutical tablets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050187
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  • 7
    Electronic Resource
    Electronic Resource
    Monoclonal antibodies against calcitonin (1988)
    Springer
    Histochemistry and cell biology 88 (1988), S. 113-125 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Twenty-seven monoclonal antibodies (MAbs) to synthetic human calcitonin (CT) were characterized for their reactivities with human CT peptide fragments by dotblot analysis on nitrocellulose paper. Most of the antibodies bound to the C-terminus and fewer to the mid-region of CT. We have studied thyroid tissue specimens from several animal species after fixation in paraformaldehyde-, glutaraldehyde-or picric acid-containing mixtures and cryostat sectioning or embedment in paraffin or plastic (Epon 812 or Lowicryl 4KM) using this panel of MAbs. The site of antigen-antibody reaction was revealed either by immunoperoxidase, immunoalkaline phosphatase or by silver-enhanced immunogold staining methods. All MAbs were able to localize CT in human, rat and mouse thyroid C cells. Nineteen MAbs recognizing synthetic salmon CT and synthetic [Asu1,7]-eel CT by bot-blot, reacted with chicken ultimobranchial body C cells. One MAb recognizing native porcine CT by dot-blot, stained C cells in hog thyroid. Immunopositivity was confined to the cytoplasm and ultrastructural immunogold labelling demonstrated that cytoplasmic secretory granules were stained. Surgical specimens from human medullary thyroid carcinoma were also analysed for the presence of CT and a variable number of positive cells was found. Furthermore, Congo red-positive areas were shown to react with the MAbs. All conventional staining and immunoabsorption controls were negative. Hence, these MAbs may be suitable for use in routine immunopathological diagnosis of CT-producing tumors and for immunocytochemical localization of the three major CT variants in different animal species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493293
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  • 8
    Electronic Resource
    Electronic Resource
    Circular and linear DNA molecules in the Entamoeba histolytica complex molecular karyotype (2000)
    Springer
    European biophysics journal 29 (2000), S. 48-56 
    Details
    ISSN: 1432-1017
    Keywords: Key wordsEntamoeba histolytica ; Linear and circular DNA ; Migration velocities ; Reorientation time ; Pulsed field
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Entamoeba histolytica genome was analysed by pulsed field gel electrophoresis under conditions to separate linear chromosomes in the 170–1400 kb range. We identified linear DNA molecules of 227, 366, 631, 850, 1112 and 1361 kb (mean sizes obtained by three different methods) and we estimated their reorientation times and migration velocities at various experimental conditions. DNA shift mobility assays, using ethidium bromide, suggested that bands migrating at 227 and 631 kb contain linear and circular DNA, whereas a band at 436 kb has only circular DNA. We obtained a regression equation relating sizes of supercoiled DNA molecules with their migration velocities during a pulse at constant electric field and temperature. We also developed a computer program (EHPATTERNS) that predicts the migration per pulse and the resolution order of circular and linear E. histolytica DNA at different pulse times and constant driving and frictional forces. The simulation showed that linear DNA molecules frequently co-migrate with circular molecules, but circular molecules change when the pulse time varies. This molecular mixture generates broad bands and difficulties in the interpretation of the molecular karyotype of E. histolytica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002490050250
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  • 9
    Electronic Resource
    Electronic Resource
    Evidence for an activating substance related to autolysis in Myxococcus coralloides D (1983)
    Springer
    Archives of microbiology 134 (1983), S. 164-166 
    Details
    ISSN: 1432-072X
    Keywords: Autolysis ; Myxococcus coralloides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When cells of Myxococcus coralloides D grow as a suspension in liquid media the cultures enter a lysis phase after having reached a critical level of cell density. Supernatants of prelytic cells added to fresh cultures are able to induce autolysis quickly. If prelytic cells are transferred to supernatants of fresh cultures, autolysis is retarded; thus delay is also observed when prelytic cells are transferred to fresh medium at the same cellular density. In the sequence of events resulting in cell lysis an activating substance may be involved. This substance may be excreted into the medium. The substance seems to be stable at high temperatures, extreme pH values and enzymatic treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407952
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  • 10
    Electronic Resource
    Electronic Resource
    Hydroxyapatite coatings: a comparative study between plasma-spray and pulsed laser deposition techniques (1997)
    Springer
    Journal of materials science 8 (1997), S. 861-865 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract A comparative study between hydroxyapatite coatings produced by two different techniques, plasma spray (PS) and pulsed-laser deposition (PLD) was carried out. Plasma spray is currently commercially used for coating dental and orthopaedical implant devices, and pulsed-laser deposition (or laser-ablation deposition) gave good results in the field of high critical temperature superconductive thin films, and is being applied to produce calcium phosphate coatings for biomedical purposes. X-ray diffraction was used to control the crystallinity of the coatings, scanning electron microscopy for the surface and cross-sectional morphology, and the pull test to determine the tensile strength of the coatings. This study reveals that the pulsed-laser deposition technique appears to be a very good candidate to replace the plasma spray in many biomedical applications, because it overcomes most of the drawbacks of the plasma spray.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018549720873
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