ISSN:
1089-7623
Source:
AIP Digital Archive
Topics:
Physics
,
Electrical Engineering, Measurement and Control Technology
Notes:
We describe a device designed to perform a fast mixing of two solutions inside the magnet of a nuclear magnetic resonance (NMR) spectrometer. The apparatus is totally nonmagnetic; it operates via a system of pneumatic valves, which drives a syringe to inject the first solution into the NMR tube that already contains the other solution. An important point is that before and after the injection, the solution in the NMR tube is free of any perturbing injecting device. The injection is controlled by the spectrometer software in the NMR pulse sequence. Simple experiments have been performed to assess the viability of the device, and evaluate the dead time after injection, as well as the loss of homogeneity and the mixing capabilities. The device described can mix up to 200 μl of solution with the solution already present in the NMR tube in less than 50 ms, with a negligible loss of homogeneity during injection. The device was specifically designed for protein folding studies but can find a general purpose for chemical reactions involving small amounts of compounds. © 2000 American Institute of Physics.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1063/1.1150602
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