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  • Senescence  (4)
  • 42.65  (2)
  • Springer  (6)
  • American Institute of Physics (AIP)
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  • Springer  (6)
  • American Institute of Physics (AIP)
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  • 1
    Electronic Resource
    Electronic Resource
    Lariat RNA of a group II intron in a filamentous fungus (1987)
    Springer
    Current genetics 12 (1987), S. 291-295 
    Details
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Group II intron ; Lariat RNA ; Senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In senescing strains of the filamentous ascomycete Podospora anserina, the first intron (il) of the mitochondrial gene for cytochrome-c-oxidase subunit I (CO I), a group II intron, accumulates as a circular mitochondrial plasmid (plDNA). In both juvenile and senescent wild type strain s two highly abundant transcripts were detected homologous to il and plDNA. In this report we show that these RNAs are identical molecules having different conformations: the first is a lariat, the second a corresponding linear molecule probably resulting from breakage of the branched circular form. Our findings suggest that the transcripts arise from processing of CO I pre-mRNA, including il, rather than from transcription of the excised plasmid. The significance of the lariat RNA concerning plDNA amplification via a postulated reverse transcription mechanism is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435292
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  • 2
    Electronic Resource
    Electronic Resource
    Transformation to senescence with plasmid like DNA in the ascomycete Podospora anserina (1980)
    Springer
    Current genetics 2 (1980), S. 181-184 
    Details
    ISSN: 1432-0983
    Keywords: Transformation ; Senescence ; Podospora anserina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the ascomycete Podospora anserina senescence through strain aging is under nucleo-cytoplasmic control and inducible in juvenile mycelia by an ‘infective principle’ transferred after cytoplasmic contact via anastomoses. A specific DNA called plasmid-like (pl) DNA, present exclusively in aging mycelia, was found to be identical with this ‘infective principle’, since it was possible to transform juvenile protoplasts to senescence by using purified p1DNA. Therefore a specific function may be attributed to this ccc DNA. Its direct involvement in a genetically programed senescence is confirmed and its development as a vector for transfer of genetic information in eukaryotes can be undertaken.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435683
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  • 3
    Electronic Resource
    Electronic Resource
    Development of a eukaryotic cloning system in Podospora anserina (1982)
    Springer
    Current genetics 6 (1982), S. 219-222 
    Details
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Eukaryotic cloning system ; Senescence ; Long-lived mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In developing a system for molecular cloning with the Podospora anserina plasmid (p1DNA) it is necessary to find recipient strains which are resistant to p1DNA mediated senescence. Three long lived double mutants which fail to exhibit spontaneous aging were genetically and biochemically analysed. All mutants were infected with p1DNA. The mutant ca viv became irreversibly senescent and therefore was not further tested. The second mutant, gr viv showed some symptoms of aging but never died. The third strain i viv remained resistant to aging from p1DNA infection and has thus proven to be the best host strain available for molecular cloning in this system. A DNA analysis of the latter two strains revealed: 1. There is no difference from the wild strain with respect to the structure of mtDNA and the integration site of the p1DNA. 2. Of the two strains, only i viv contains free p1DNA in its mitochondria but in low amounts if compared to the wild strain. These experimental results are interpreted as follows: 1. The gr viv strain does not liberate spontaneously the p1DNA from mtDNA, but following infection is able to replicate and express this plasmid and therefore is a potential host for transformation. 2. The i viv strain liberates the mitochondrial plasmid but does not express senescence even when infected with p1DNA. Therefore, this strain is an ideal recipient for transformation provided a marker other than senescence is cloned.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390341
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  • 4
    Electronic Resource
    Electronic Resource
    The onset of senescence is affected by DNA rearrangements of a discontinuous mitochondrial gene in Podospora anserina (1985)
    Springer
    Current genetics 9 (1985), S. 373-382 
    Details
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Rearrangements of mtDNA ; Senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mapping and transcription studies have revealed that in Podospora anserina the causative agent of senescence, a mitochondrial plasmid (p1DNA), is identical with intronl of the discontinuous gene for cytochrome-c-oxidase subunit 1 (COI), which is 2 kpb from the discontinuous gene for cytochrome b (Cytb). A mitochondrial mutant (ex1) devoid of the COI, but not of the Cytb gene provides longevity. A molecular model for the onset of senescence is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421608
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  • 5
    Electronic Resource
    Electronic Resource
    The role of the biexciton in a dynamic density matrix theory of the semiconductor band edge (1994)
    Springer
    The European physical journal 93 (1994), S. 205-211 
    Details
    ISSN: 1434-6036
    Keywords: 42.65 ; 71.35
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We describe the dielectric response of the semiconductor band edge in a dynamic density matrix model. Our treatment is based on a set of χ3-relevant constitutive equations involving two-, four- and six-point density matrices. We demonstrate that under certain conditions all contributions to the third order susceptibility can be expressed in terms of excitonic and biexcitonic transitions. As a first application of these χ3-relevant equations we investigate the influence of the biexciton on the optical Stark effect in CuCl. We calculate shifts and lineshapes. Our results turn out to be in excellent agreement with experiments as well as with other theoretical predictions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01316964
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  • 6
    Electronic Resource
    Electronic Resource
    A dynamics-controlled truncation scheme for the hierarchy of density matrices in semiconductor optics (1994)
    Springer
    The European physical journal 93 (1994), S. 195-204 
    Details
    ISSN: 1434-6036
    Keywords: 42.65 ; 71.35 ; 71.45
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We discuss the interaction of coherent electromagnetic fields with the semiconductor band edge in a dynamic density matrix model. Due to the influence of the Coulomb-interaction then-point density matrices are coupled in an infinite hierarchy of equations of motion. We show how this hierarchy is related to an expansion of the density matrices in terms of powers of the exciting field. We make use of the above results to set up a closed set of equations of motion involving two-, four-and six-point correlation functions, from which all third order contributions to the polarization can be calculated exactly. Comparison of our treatment of the hierarchy with the widely used RPA decoupling on the two-point level, gives interesting insight into the validity of the RPA. In particular we find, that a RPA-like factorization for two of the relevant density-matrices yields a solution of their respective equations of motion to lowest order in the electric field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01316963
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