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  • Chemistry  (5)
  • Wiley-Blackwell  (5)
  • American Institute of Physics (AIP)
  • Springer
  • 1
    Electronic Resource
    Electronic Resource
    Production of recombinant proteins in transgenic plants: Practical considerations (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 56 (1997), S. 473-484 
    Details
    ISSN: 0006-3592
    Keywords: transgenic plants ; recombinant protein ; gene expression ; downstream processing ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This review is based on our recent experience in producing the first commercial recombinant proteins in transgenic plants. We bring forward the issues that have to be considered in the process of selecting and developing a winning transgenic plant production system. From the production point of view, transcription, posttranscription, translation, and posttranslation are important events that can affect the quality and quantity of the final product. Understanding the rules of gene expression is required to develop sound strategies for optimization of recombinant protein production in plants. The level of recombinant protein accumulation is critical, but other factors such as crop selection, handling and processing of transgenic plant material, and downstream processing are equally important when considering commercial production. In some instances, the cost of downstream processing alone may determine the economic viability of a particular plant system. Some of the potential advantages of a plant production system such as the high levels of accumulation of recombinant proteins, glycosylation, compartmentalization within the cell, and natural storage stability in certain organs are incentives for aggressively pursuing recombinant protein production in plants. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 473-484, 1997.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    DTA for control of stability in polyolefin wire and cable compounds (1973)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 13 (1973), S. 429-434 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Differential thermal analysis offers a convenient and rapid way of determining stabilization levels in polyolefin compositions. Since it is normally used at temperatures in the fabrication range, it provides a direct readout of processing stability. At the same time, because the chemistry of the polymer system at these temperatures may be quite different from that at normal use temperatures, judgment must be exercised in attempting to extrapolate high temperature data. New factors can come in at either end of the temperature range which are inoperative at the other extreme.The experimental procedures used are described, and the special precautions necessary for operation down to the 140-150°C range indicated. Problems arising from sample inhomogeneities are discussed, and the critical importance of stabilizer migration in the solid state pointed out, including invalidation of extrapolation down to normal temperatures of data taken above the melting range.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760130606
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  • 3
    Electronic Resource
    Electronic Resource
    Use of the spectrophotometer to control carbon dispersion quality in polyethylene (1966)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 6 (1966), S. 217-226 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Renewed interest in the development of a quantitative optical technique for assessing the quality of carbon dispersion in weather-resistant polyethylene compositions has given fresh significance to the findings of a study conducted earlier by a task group of ATSM Committee D-20 but not previously reported in the literature. This group evolved and refined a spectrophotometric procedure which was shown to be capable of good interlaboratory reproducibility when the same film was measured by each of the participants in turn. When each laboratory prepared its own film specimen from a standard lot of pellets, however, this reproducibility dropped to a very unsatisfactory level. It was concluded that fine-grained inhomogeneities even in compounds considered to have excellent dispersion quality preclude reproducible test results when sample weights of only a few milligrams are involved. The problem is aggravated by the difficulty of preparing thin, uniform films and complicated by a nonlinear dependence of transmission on film thickness.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760060308
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  • 4
    Electronic Resource
    Electronic Resource
    Why do plastics stress-crack? (1965)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 5 (1965), S. 125-129 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Possible applicability of Griffith's theory to stress-cracking in microcrystalline organic polymers is considered. Although the time-dependent nature of the phenomenon and the plastico-elastico-viscous character of the medium make such application debatable, it is found that this approach in combination with the contributions of others notably Rebinder and his associates, can provide rationalization for many of the empirical facts. The observed “case-hardening” action of surfactants on specimens under stress suggests that the mobility of polymer chain segments in surface layers may in fact be restricted under these conditions as theorized, facilitating local concentration of stresses to a level exceeding the strength of the material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760050307
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  • 5
    Electronic Resource
    Electronic Resource
    Processing of transgenic corn seed and its effect on the recovery of recombinant β-glucuronidase (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 60 (1998), S. 44-52 
    Details
    ISSN: 0006-3592
    Keywords: β-glucuronidase ; recovery ; recombinant enzyme ; protein extraction ; transgenic corn ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The tools of plant biotechnology that have been developed to improve agronomic traits are now being applied to generate recombinant protein products for the food, feed, and pharmaceutical industry. This study addresses several processing and protein recovery issues that are relevant to utilizing transgenic corn as a protein production system. The gus gene coding for β-glucuronidase (rGUS) was stably integrated and expressed over four generations. The accumulation level of rGUS reached 0.4% of total extractable protein. Within the kernel, rGUS was preferentially accumulated in the germ even though a constitutive ubiquitin promoter was used to direct gus expression. Fourth-generation transgenic seed was used to investigate the effect of seed processing on the activity and the recovery of rGUS. Transgenic seed containing rGUS could be stored at an ambient temperature for up to two weeks and for at least three months at 10°C without a significant loss of enzyme activity. rGUS exposed to dry heat was more stable in ground than in whole kernels. The enzyme stability was correlated with the moisture loss of the samples during the heating. Transgenic seed was dry-milled, fractionated, and hexane extracted to produce full-fat and defatted germ fractions. The results of the aqueous extraction of rGUS from ground kernels, full-fat germ, and defatted-germ samples revealed that approximately 10 times more rGUS per gram of solids could be extracted from the ground full-fat germ and defatted-germ than from the kernel samples. The extraction of corn oil from ground germ with hot hexane (60°C) did not affect the extractable rGUS activity. rGUS was purified from ground kernels and full-fat germ extracts by ion exchange, hydrophobic interaction, and size exclusion chromatography. Similar purity and yield of rGUS were obtained from both extracts. Biochemical properties of rGUS purified from transgenic corn seed were similar to those of E. coli GUS. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 44-52, 1998.
    Additional Material: 6 Ill.
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