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  • Cell & Developmental Biology  (29)
  • Analytical Chemistry and Spectroscopy  (21)
  • Wiley-Blackwell  (50)
  • American Institute of Physics (AIP)
  • Blackwell Publishing Ltd
  • Cambridge University Press
Collection
Keywords
Publisher
  • Wiley-Blackwell  (50)
  • American Institute of Physics (AIP)
  • Blackwell Publishing Ltd
  • Cambridge University Press
Years
  • 1
    Electronic Resource
    Electronic Resource
    Early development of the kelp fly, Coelopa frigida (Diptera). II. Morphology of cleavage and blastoderm formationDedicated to Prof. Dr. F. Seidel in commemoration of his 75th birthday. (1973)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 141 (1973), S. 235-255 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cleavage and blastoderm formation in Coelopa frigida are extremely rapid developmental processes. In short (6-7 minutes) successive cell cycles, nuclei multiply and spread out through the egg. The movement seems to be aided by endoplasmic vesicles and cisternae which are in direct contact with the nuclear membrane. The first cells to separate from the egg plasmodium in early superficial cleavage stages are the pole cells.Precursor material from multivesicular bodies forms the pole cell membranes. The primary nuclei from the posterior pole region are removed from the blastoderm by the pole cell segregation. Blastoderm nuclei from the regions adjacent to the posterior pole migrate into the residual periplasm after pole cell segregation has been completed and constitute the blastoderm nuclei in that region of the egg. Nucleoli are not revealed during internal cleavage. They appear in pole cells shortly after their segregation. The generation time of the blastoderm nuclei increases after the twelfth cleavage. Concurrently, nucleoli form in the blastoderm nuclei and permanent cell membranes separate individual blastoderm cells. After blastoderm cells have been separated from each other, they remain in contact with the interior yolk sac by means of cytoplasmic canals. This contact is maintained at least during the early phases of blastokinesis. Observations on nuclear migration and rapid membrane formation are discussed as examples of protein assembly from subunits as an alternative to de novo protein synthesis in early stages of development.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051410210
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  • 2
    Electronic Resource
    Electronic Resource
    An improved ion-pairing reagent for HPLC (1981)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 584-585 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Reverse-phase mode ; TBAOAc/NH4OAc ion-pairing reagent circumvents problems ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240041111
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  • 3
    Electronic Resource
    Electronic Resource
    Biodegradation of Pharmaceutical Residues Investigated by SPE-GC/ITD-MS and On-Line Derivatization (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 474-478 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Solid-phase extraction, on-line derivatization, and measurement by ion trap mass spectrometry (ITD-MS) were used to investigate the biological degradation of pharmaceutical residues (clofibric acid, ibuprofen, diclofenac). The results of the single steps of sample pretreatment and analytical determination are reported. MS/MS measurements were performed on an ITD-MS by selecting collision induced dissociation of the molecular ions (M+) as parent ions to defined daughter ions. A pilot sewage plant and biofilm reactors operating under oxic and anoxic conditions were run as model systems with synthetic sewage water containing 10 to 50 mg/L dissolved organic carbon (DOC) and pharmaceuticals in concentrations of 10 μg/L. Clofibric acid displayed its persistent character in all cases. The pilot sewage plant and the oxic biofilm reactor showed comparable results for diclofenac and ibuprofen, which both were partly degraded. These results can explain the occurrence of these substances in sewage effluents and in the aquatic environment. A high degree of degradation was found especially for ibuprofen in the oxic biofilm reactor, which was attributed to adaptation of the biofilm to the residue. Two metabolites of ibuprofen could be identified on the basis of their mass spectra and comparison with literature data, viz. hydroxyibuprofen and carboxyibuprofen.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Capillary zone electrophoresis of neutral organic molecules in organic - aqueous solution (1994)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 17 (1994), S. 713-718 
    Details
    ISSN: 0935-6304
    Keywords: Electrophoretic capillary chromatography ; Organic modifiers ; Hydrophobic association ; Capacity factors ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Very good separations of non-ionic organic compounds have been achieved by capillary zone electrophoresis (CZE) using a tetrahep-tylammonium salt as an additive in aqueous acetonitrile as solvent. A systematic study was undertaken to determine the effect of experimental parameters on electroosmotic mobility and electro-phoretic mobility. It was found that pH, acetonitrile concentration, and the type and concentration of quaternary ammonium salt are important experimental variables. Under appropriate conditions, the separation window was enlarged and a broad range of electrically neutral organics, including very hydrophobic compounds (e. g. poly-cyclic aromatic hydrocarbons) and fairly hydrophilic compounds were separated in a relatively short time. By adjusting the separation conditions, high resolution CZE of a specific group of neutral organic compounds could be achieved. A method for calculation of capacity factor was proposed and capacity factors for a variety of non-lonic organic compounds were calculated.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240171006
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  • 5
    Electronic Resource
    Electronic Resource
    Silicalite as a stationary phase for HPLC (1996)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 19 (1996), S. 691-695 
    Details
    ISSN: 0935-6304
    Keywords: HPLC ; Molecular sieve ; Cis-trans isomers ; Phenols ; Selectivity ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A molecular sieve known as Silicalite was used as the column packing for HPLC. Silicalite contains channels (or cavities) approximately 6 Å in diameter but, unlike most other molecular sieves, Silicalite is hydrophobic. The retention times of methyl ketones and substituted phenols containing n-alkyl groups increase with increasing chain length of the substituent. However, phenols with very bulky substituents appear to be excluded from the Silicalite channels and elute very quickly. Excellent separations were obtained for a number of compounds with only slight differences in chemical structure. These include phenol isomers with a primary- or secondary alkyl group, position isomers of substituted phenols, and aliphatic cis-trans isomers.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240191207
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  • 6
    Electronic Resource
    Electronic Resource
    Separation of metal cations in acidic solution by capillary electrophoresis with direct and indirect UV detection (1997)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 20 (1997), S. 653-656 
    Details
    ISSN: 0935-6304
    Keywords: Capillary electrophoresis ; Metal cations ; Acid solution ; UV detection, direct and indirect ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Methods for the detectionn of metal cations under acidic conditions, near PH 2, in capillary electrophoresis (CE) were investigatged. Conditions for direct UV detection of UV absorbing metal cations such as Cr3+, Cu2+, Fe3+, UO22+, VO2+, and VO2+ were established With aqueous HCl or HClO4 as the electroyte carrier. The speciation of vanadium(IV) and vanadium(V) at PH 2.3 by CE was achieved with direct detection at 185 nm. With the strong absorbance at 185 nm, no complexation was needed to detect the metal cations. An indirect UV detrection scheme for acidic conditions was also investigated. Several background carrier electolytes (BCES) were studied including 4-methylbenzylamince, nicontinamide, pyridazine, guanidine, 3-picoline, and chromium (III) to determine their effectivencess under very acidic conditions. The efect of ioni c surfactants and the nonionic surfactant, Trition X-100, on the peak heights and N Values was also studied.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240201207
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  • 7
    Electronic Resource
    Electronic Resource
    The contrarian viewpoint (1992)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 15 (1992), S. 569-569 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240150902
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of sample diluents on peak broadening in gas chromatography (1988)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 11 (1988), S. 534-536 
    Details
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; Split injector ; Solvent effect ; Peak broadening ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240110709
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  • 9
    Electronic Resource
    Electronic Resource
    Analysis of volatile halocarbons in water by GC-ECD involving direct on-column injection of the sample (1990)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 13 (1990), S. 561-564 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240130807
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  • 10
    Electronic Resource
    Electronic Resource
    Extracellular matrix: Differential influence on growth and biosynthesis patterns of vascular smooth muscle cells from SHR and WKY rats (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 141 (1989), S. 267-274 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Smooth muscle cells from spontaneously hypertensive rats (SHR) elaborated extracellular matrix (ECM) material in culture that was more stimulatory to growth of cells from normotensive (WKY) animals than their own matrix. Both cell types elaborated ECMs consisting of glycoconjugate material (proteoglycans, glycopeptides) elastin, and collagens, but there were differences in the relative proportions of the compounds synthesized. Cells from SHR produced an ECM richer in elastin than that synthesized by WKY derived cells (∼19% vs. 11%, respectively). However, the latter elaborated ECMs containing more (∼45% for WKY vs. 29% for SHR) glycoconjugate material than the former. The lysyloxidase-mediated crosslinking of elastin was more rapid in cultured cells from SHR animals than from their normotensive counterparts and may be as a consequence of increased substrate (tropoelastin) availability in ECMs from SHR animals. The relative proportions and sulphate levels of the glycosaminoglycans associated with matrix material elaborated by the two cell types were similar. Radiolabelled glycoconjugate material was degraded by cells (SHR/WKY) when they were plated upon pre-formed ECMs, and their patterns of synthesis of new matrix was markedly altered under such conditions. New matrix material elaborated by cells plated upon ECM-coated dishes consisted predominantly of glycopeptide and proteoglycan matrix components. Epidermal growth factor promoted the incorporation of [3H]-thymidine into DNA by quiescent cells, and this was also markedly stimulated when cells were plated onto ECM-coated plasticware rather than onto plastic substratum.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041410206
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