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  • Epidermis  (2)
  • HPLC  (2)
  • Springer  (4)
  • American Institute of Physics (AIP)
  • American Physical Society (APS)
  • Oxford University Press
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 11 (1985), S. 383-395 
    ISSN: 1573-1561
    Keywords: HPLC ; retention time ; area ratio ; phenolic acids ; flavonoids ; soybean ; Glycine max
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Relative retention times and wavelength area ratios for over 50 standard compounds were calculated using reverse-phase HPLC. The standard compounds analyzed included benzoic acids, cinnamic acids, benzene carboxylic acids, acetic acids, coumarins, benzaldehydes and a variety of flavonoid compounds including flavanones, flavones, isoflavones, and their glycosides. Each standard compound was chromatographed by three different gradient elutions. Compounds were detected by UV absorption at 254 nm and 280 nm. Relative retention times with respect to two different internal references and the 254nm: 280nm wavelength area ratio was determined for each standard. Soybean root and seed extracts were analyzed for the presence of the standard compounds using the chromatographic conditions described.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 9 (1983), S. 1281-1292 
    ISSN: 1573-1561
    Keywords: Allelopathy ; roots ; soybean fingerprint ; growth stage ; HPLC ; soybeans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract HPLC was used to obtain fingerprint patterns of organic compounds extracted from roots of five soybean varieties at seven stages of growth. Fifteen major peaks were observed in all varieties at most growth stages. With increasing stage of growth, the less polar compounds accounted for a greater relative percentage of total peak area. Concentration of compounds extracted from roots increased dramatically as plants approached nodulation and flowering and then decreased to initial levels as plants matured.
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  • 3
    ISSN: 1615-6102
    Keywords: Epidermis ; Golgi apparatus ; Nudibranchia ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The epidermal cell layer of the apical end of the ceras was investigated in two species of aeolid nudibranchs. Based on cellular inclusions, mostly two cell types were found: mucoid and ellipsoid-vacuolate cells. Mucoid cells ofCoryphella rufibranchialis have large heterogeneous and fibrillar secretory granules whereas inAeolidia papillosa, the granules are homogeneous, but vary in electron density from one cell to another. Ellipsoid-vacuolate cells contained large quantities of small vacuoles with an included ellipsoidal structure. Both species contained very numerous ellipsoid-vacuolate cells. Secretory granules and ellipsoid-vacuoles appear to arise from the Golgi apparatus and these contents stain with PAS, suggesting a polysaccharide composition. Mucoid cells contained both secretory granules and ellipsoid-vacuoles which may arise from the same Golgi apparatus.
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  • 4
    ISSN: 1615-6102
    Keywords: Cytochemistry ; Epidermis ; Golgi apparatus ; Mucoid cells ; Nudibranchia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The epidermal mucoid cells in the cerata ofAeolidia papillosa andCoryphella rufibranchialis were cytochemically tested to determine the composition of their secretory products. The PAS, alcian blue, PA-TSC-SP and high iron diamine stains were used to determine the presence of acidic, sulphated, and periodate-reactive groups on the mucopolysaccharides. The secretory granules in mucoid cells ofA. papillosa consisted of periodate-reactive mucopolysaccharides with acid groups other than sulphates. Each granule also contained fibrils which were not oxidized by periodic acid. The mucoid secretory granules inC. rufibranchialis contained weakly acidic sulphated mucopolysaccharides, but no periodate-reactive polysaccharide groups. In both aeolid nudibranchs the Golgi apparatus at the base of the mucoid cells contained products which stained the same as those in the secretory granules, confirming that the Golgi apparatus is involved in the synthesis of mucoid secretory products. In addition the mucoid cell Golgi complex ofC. rufibranchialis also gave rise to ellipsoid vacuoles which contained sulphated mucopolysaccharides.
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