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  • 1D trNOESY-TOCSY  (1)
  • Climate change  (1)
  • Springer  (2)
  • American Institute of Physics
  • Copernicus
  • Public Library of Science
Collection
Keywords
Publisher
  • Springer  (2)
  • American Institute of Physics
  • Copernicus
  • Public Library of Science
Years
  • 1
    Electronic Resource
    Electronic Resource
    Impact of long-term drought on xylem embolism and growth in Pinus halepensis Mill. (1998)
    Springer
    Trees 12 (1998), S. 187-195 
    Details
    ISSN: 0931-1890
    Keywords: Key words Aleppo pine ; Climate change ; Water stress ; Cavitation ; Transpiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The present study was carried out to elucidate the response mechanisms of 50-year-old Pinus halepensis Mill. trees to a long-term and severe drought. The amount of water available to trees was artificially restricted for 12 months by covering the soil with a plastic roof. Over the short term a direct and rapid impact of drought was evident on the water relations and gas exchanges of trees: as the soil dried out in the Spring, there was a concurrent decrease of predawn water potential; transpiration was strongly reduced by stomatal closure. Seasonal changes in the water volume fractions of twig and stem xylem were observed and interpreted as the result of cavitation and refilling in the xylem. When droughted trees recovered to a more favourable water status, refilling of embolized xylem was observed; twig predawn water potentials were still negative in the period when the embolism was reversed in the twig xylem. A few months after the removal of the covering, no differences in whole plant hydraulic resistance were observed between droughted and control trees. Needle and shoot elongation and stem radial growth were considerably reduced in droughted trees; no strategy of trees to allocate carbon preferentially to the stem conducting tissues was apparent throughout the experiment. An after-effect of the drought on growth was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00009709
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  • 2
    Electronic Resource
    Electronic Resource
    Application of homonuclear 3D NMR experiments and 1D analogs to study the conformation of sialyl Lewisx bound to E-selectin (1997)
    Springer
    Journal of biomolecular NMR 9 (1997), S. 423-436 
    Details
    ISSN: 1573-5001
    Keywords: 3D TOCSY-trNOESY ; 1D TOCSY-trNOESY ; 1D trNOESY-TOCSY ; trNOE ; trROE ; E-selectin ; sialyl Lewisx
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The conformation of the sialyl Lewis x tetrasaccharidebound to E-selectin was previously determined from transfer NOE (trNOE)experiments in conjunction with a distance-geometry analysis. However, theorientation of the tetrasaccharide ligand in the binding site of E-selectinis still unknown. It can be predicted that the accurate quantitativeanalysis of all trNOEs, including those originating from spin diffusion, isone key to analyze the orientation of sialyl Lewisx in thebinding pocket of E-selectin. Therefore, we applied homonuclear 3D NMRexperiments and 1D analogs to obtain trNOEs that could not unambiguously beassigned from previous 2D trNOESY spectra, due to severe resonance-signaloverlap. A 3D TOCSY-trNOESY experiment, a 1D TOCSY-trNOESY experiment, and a1D trNOESY-TOCSY experiment of the sialyl Lewisx/E-selectincomplex furnished new interglycosidic trNOEs and provided additionalinformation for the interpretation of trNOEs that have been describedbefore. A 2D trROESY spectrum of the sialyl Lewisx/E-selectincomplex allowed one to identify the amount of spin-diffusion contributionsto trNOEs. Finally, an unambiguous assignment of all trNOEs, and an analysisof spin-diffusion pathways, was obtained, creating a basis for aquantitative analysis of trNOEs in the sialylLewisx/E-selectin complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018358929268
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