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  • Column liquid chromatography  (443)
  • Wheat  (174)
  • Springer  (617)
  • American Institute of Physics
  • Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
  • International Union of Crystallography
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  • Springer  (617)
  • American Institute of Physics
  • Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
  • International Union of Crystallography
  • Elsevier  (10)
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Years
  • 1
    Electronic Resource
    Electronic Resource
    Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation (1995)
    Springer
    Plant cell reports 15 (1995), S. 159-163 
    Details
    ISSN: 1432-203X
    Keywords: Wheat ; transformation ; glyphosate ; CP4/GOX ; embryos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lack of alternative selectable markers in crop transformation has been a substantial barrier for commercial application of agricultural biotechnology. We have developed an efficient selection system for wheat transformation using glyphosate-tolerant CP4 and GOX genes as a selectable marker. Immature embryos of the wheat cultivar Bobwhite were bombarded with two separate plasmids harboring the CP4/GOX and GUS genes. After a 1 week delay, the bombarded embryos were transferred to a selection medium containing 2 mM glyphosate. Embryo-derived calli were subcultured onto the same selection medium every 3 weeks consecutively for 9–12 weeks, and were then regenerated and rooted on selection media with lower glyphosate concentrations. Transgenic plants tolerant to glyphosate were recovered. ELISA assay confirmed expression of the CP4 and GOX genes in R0 plants. Southern blot analysis demonstrated that the transgenes were integrated into the wheat genomes and transmitted to the following generation. The use of CP4 and GOX genes as a selectable marker provides an efficient, effective, and alternative transformation selection system for wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193711
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic diversity in European wheat and spelt breeding material based on RFLP data (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 994-1003 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Spelt ; RFLP ; Marker Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fifty-two winter wheat (Triticum aestivum L.), nine spring wheat, and 20 spelt (Triticum spelta L.) lines representing part of the European breeding germplasm, were assayed for RFLPs (restriction fragment length polymorphisms) with 56 wheat DNA clones and two barley cDNA clones. Objectives of this study were to (1) determine the level of variation for RFLPs in the wheat and spelt breeding lines, (2) characterize the genetic diversity within the European winter wheat germplasm, and (3) evaluate the usefulness of RFLP markers for pedigree analysis and the grouping of wheat and spelt lines of various origins. Seventy-three of the 166 RFLP loci detected with 58 probes and one restriction enzyme were polymorphic for the 81 lines. The percentage of polymorphic loci was greatest for the B genome (58%) and smallest for the D genome (21%). Among the 81 lines, 271 different RFLP bands were detected. RFLP band frequencies of the winter wheat lines differed considerably (≥0.5) from those of the spring wheat lines at five loci, and from those of the spelt lines at 17 loci. Eight cultivars that had a major impact as progenitors on the development of improved winter wheat cultivars accounted for 93% of the observed RFLP bands in winter wheat. Genetic distance (GD) estimates between two lines ranged between 0.01 and 0.21. Mean GD estimates within winter wheat (0.083), within spring wheat (0.108) and within spelt (0.096) were smaller than between spring and winter wheat (0.114), and greatest between winter wheat and spelt (0.132) and spring wheat and spelt (0.148). Principal coordinate analysis performed on GD estimates revealed a clear separation of wheat and spelt germplasm. Novel spelt lines with various proportions of wheat germplasm were positioned between wheat and traditional spelt lines. The spring wheat lines formed a distinct group at the periphery of the distribution of the winter wheat lines. Subgroupings of the winter wheat lines according to the cluster analysis were in good agreement with their origin, and lines with common ancestors were grouped together.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220807
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 982-990 
    Details
    ISSN: 1432-2242
    Keywords: Leaf rust ; RFLP ; RAPD ; Wheat ; Agropyron elongatum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this study was to identify molecular markers linked to the wheat leaf rust resistance gene Lr24 derived from Agropyron elongatum (3DL/3Ag translocation). Two near isogenic lines (NILs), ‘Arina’ and Lr24/7 * “Arina”, were screened for polymorphism at the DNA level with 115 RFLP probes. Twenty-one of these probes map to the homoeologous group 3. In addition, 360 RAPD primers were tested on the NILs. Six RFLP probes showed polymorphism between the NILs, and 11 RAPD primers detected one additional band in the resistant NIL. The genetic linkage of the polymorphic markers with Lr24 was tested on a segregating F2 population (150 plants) derived from a cross between the leaf rust resistant Lr24/7 * “Arina” and the susceptible spelt (Triticum spelta) variety ‘Oberkulmer’. All 6 RFLP markers were completely linked to Lr24: one was inherited as a codominant marker (PSR1205), one was in coupling phase (PSR1203) and 4 were in repulsion phase (PSR388, PSR904, PSR931, PSR1067) with Lr24. The localization of these probes on chromosome 3D was confirmed by nulli-tetrasomic analysis. Distorted genotypic segregation was found for the Codominant RFLP marker PSR1205. This distortion can be explained by the occurrence of hemizygous plants. One of the 11 RAPD markers (OPJ-09) also showed complete linkage to theLr24 resistance gene. The polymorphic RAPD fragment was cloned and sequenced. Specific primers were synthesized, and they produced an amplification product only in the resistant plants. This specific marker allows a reliable and rapid screening of a large number of genotypes in practical breeding. Analysis of 6 additional lines containing Lr24 revealed that 3 lines have a smaller chromosomal segment of A. elongatum than lines derived from ‘Agent’, a commonly used gene donor for the Lr24 resistance gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222911
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  • 4
    Electronic Resource
    Electronic Resource
    Linkage between RFLP markers and genes affecting kernel hardness in wheat (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 580-586 
    Details
    ISSN: 1432-2242
    Keywords: Kernel hardness ; Wheat ; RFLP ; QTL ; Puroindoline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A molecular-marker linkage map of wheat (Triticum aestivum L. em. Thell) provides a powerful tool for identifying genomic regions influencing breadmaking quality. A variance analysis for kernel hardness was conducted using 114 recombinant inbred lines (F7) from a cross between a synthetic and a cultivated wheat. The major gene involved in kernel hardness, ha (hard), known to be on chromosome arm 5DS, was found to be closely linked with the locus Xmta9 corresponding to the gene of puroindoline-a. This locus explained around 63% of the phenotypic variability but there was no evidence that puroindoline-a is the product of Ha (soft). Four additional regions located on chromosomes 2A, 2D, 5B, and 6D were shown to have single-factor effects on hardness, while three others situated on chromosomes 5A, 6D and 7A had interaction effects. Positive alleles were contributed by both parents. A three-marker model explains about 75% of the variation for this trait.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417951
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  • 5
    Electronic Resource
    Electronic Resource
    Linkage between RFLP markers and genes affecting kernel hardness in wheat (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 580-586 
    Details
    ISSN: 1432-2242
    Keywords: Key words Kernel hardness ; Wheat ; RFLP ; QTL ; Puroindoline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A molecular-marker linkage map of wheat (Triticum aestivum L. em. Thell) provides a powerful tool for identifying genomic regions influencing breadmaking quality. A variance analysis for kernel hardness was conducted using 114 recombinant inbred lines (F7) from a cross between a synthetic and a cultivated wheat. The major gene involved in kernel hardness, ha (hard), known to be on chromosome arm 5DS, was found to be closely linked with the locus Xmta9 corresponding to the gene of puroindoline-a. This locus explained around 63% of the phenotypic variability but there was no evidence that puroindoline-a is the product of Ha (soft). Four additional regions located on chromosomes 2A, 2D, 5B, and 6D were shown to have single-factor effects on hardness, while three others situated on chromosomes 5A, 6D and 7A had interaction effects. Positive alleles were contributed by both parents. A three-marker model explains about 75% of the variation for this trait.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050318
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  • 6
    Electronic Resource
    Electronic Resource
    Liquid chromatography with charge transfer complexation (1990)
    Springer
    Chromatographia 29 (1990), S. 419-428 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Acceptor-Donor complexes ; Selectivity enhancement ; Physical properties/retention correlation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The paper describes the retention changes observed when an organic acceptor is added to the mobile phase of a liquid chromatography system. The capacity factor decrease depends on the donor character of the solute. Correlations with ionization potentials, HOMO energies and half wave oxidation potentials demonstrate the role of the organic acceptor. According monstrate the role of the organic acceptor. According to the chromatographic properties of the acceptor which is either unretained (TCNE) or slightly retained (TNF) different retention mechanisms are proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02261388
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of doxycycline in small serum samples by liquid chromatography. Application to pharmacokinetical studies on small laboratory animals (1993)
    Springer
    Chromatographia 35 (1993), S. 524-526 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Doxycycline in serum ; Pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Several methods have been described for the analysis of doxycycline in serum which require at least 0.5 mL sample. The assay presented here requires small amounts of serum (0.1 mL) and shows good sensitivity. A Ultrabase C-18 column and mobile phase consisting of acetonitrile-bidistilled water (28∶72) were used, flow rate 1 mL min−1 and monitored at 350 nm. Linearity proved satisfactory between 0.025 and 2.5 μg mL−1 of serum. This assay for doxycycline showed good precision (coefficient of variation 〈6%) and allowed quantitation of serum levels as low as 25 ng mL−1 using 100 μL samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02267911
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  • 8
    Electronic Resource
    Electronic Resource
    A stability-indicating high-performance liquid chromatographic assay for the determination of some pharmaceutically important nitrocompounds (1990)
    Springer
    Chromatographia 30 (1990), S. 176-180 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Flurazolidone and other nitro-pharmaceuticals ; Quantitative analysis ; Stability tests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simple, rapid and precise stability-indicating high performance liquid chromatographic assay for the determination of furazolidone (I), nitrofurazone (II), nitrofurantoin (III), niridazole (IV) and nifuroxime (V) in pure form and in pharmaceutical preparations has been devloped. Reversed phase chromatography was conducted using a Lichrosorb R.P. 18 column (250×4 mm), with methanol-water-buffer pH3 (40∶55∶5) eluent and detection at 365, 375, 367, 368 and 340 nm respectively. Calibration graphs were linear over the concentration ranges 3–18, 1–10, 1–10, 3–18 and 3–18 μm·ml−1. Reoveries from bulk drugs were 99.94–100.00 with a relative standard deviation of 1.02–1.61. Sunlight degradation of most of the studied drugs to 5-nitrofuraldehyde was observed. Accelerated stability studies have been carried out on each drug by exposure to sunlight for different time periods. Graphs of log of the remaining concentration (Log. C) against time indicated that photodecomposition of the nitrocompounds is a first order reaction. The proposed method was applied to some representative dosage forms and the results obtained were in good agreement with those obtained by the official methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02274542
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  • 9
    Electronic Resource
    Electronic Resource
    Urine clean-up method for determination of six arsenic species by LC-AAS involving microwave assisted oxidation and hydride generation (1996)
    Springer
    Chromatographia 43 (1996), S. 507-512 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Urine clean-up ; Arsenic speciation ; Microwave assisted oxidation ; Atomic absorption spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An effective clean-up method based on the precipitation of most high molecular mass inorganic salts and organic compounds in ethanol at −15°C has been developed for the determination of arsenite, arsenate, monomethylarsonate (MMA), dimethylarsinate (DMA), arsenobetaine (AsB) and arsenocholine (AsC) in urine. Analyte recovery was close to 100%. The six species were separated on an anionic HPLC column and determined by microwave-assisted oxidation-hydride generation-atomic absorption spectrometry. The detection limits and relative standard deviation of the whole procedure were within the 8–15 μg L−1 and 2.5–5.3% ranges, respectively, which allows application to the analysis of urine of people exposed to arsenic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02293002
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  • 10
    Electronic Resource
    Electronic Resource
    Ability of the earthworms Aporrectodea rosea and Aporrectodea trapezoides to increase plant growth and the foliar concentration of elements in wheat (Triticum aestivum cv. spear) in a sandy loam soil (1994)
    Springer
    Biology and fertility of soils 18 (1994), S. 150-154 
    Details
    ISSN: 1432-0789
    Keywords: Earthworm ; Aporrectodea rosea ; Aporrectodea trapezoides ; Wheat ; Foliar concentration ; Trace elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a greenhouse study, the ability of the earthworms Aporrectodea rosea and A. trapezoides to influence the foliar concentration of elements and the growth of wheat plants was assessed 27 days after sowing in a sandy loam soil. The presence of A. rosea and A. trapezoides (at densities equivalent to 314 and 471 m-2, respectively) caused a significant increase in the shoot dry weight of wheat. The presence of A. rosea and A. trapezoides (at densities equivalent to 314 and 157 m-2, respectively) was also associated with a significant increase in the root dry weight of wheat. The presence of A. rosea caused a significant increase in the foliar concentration of Ca, Cu, K, Mn, N, Na, and P, but did not influence the foliar concentration of Al, B, Fe, Mo, Mg, S, and Zn. The presence of A. trapezoides was associated with a significant increase in the foliar concentration of Al, Ca, Fe, K, Mn, N, and Na, but did not influence the foliar concentration of B, Cu, Mo, Mg, P, S, and Zn. These results demonstrate the potential of A. rosea and A. trapezoides to increase the growth of wheat in a sandy loam soil and suggest that the mechanism by which they increased plant growth was, in part, through increasing the availability and uptake of nutrients from this soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336462
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