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  • Agrobacterium rhizogenes  (2)
  • Solanum tuberosum  (2)
  • Bioassay  (1)
  • Springer  (4)
  • American Institute of Physics
  • American Physical Society
  • Oxford University Press
Collection
Keywords
Publisher
  • Springer  (4)
  • American Institute of Physics
  • American Physical Society
  • Oxford University Press
Years
  • 1
    Electronic Resource
    Electronic Resource
    Genetic modification of potato development using Ri T-DNA (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 440-446 
    Details
    ISSN: 1432-2242
    Keywords: Agrobacterium rhizogenes ; Potato ; Somaclonal variation ; Genetic manipulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Forty-two potato plants were regenerated from a hairy-root line obtained after infection of a shoot of Solanum tuberosum cv ‘Desiree’ with Agrobacterium rhizogenes strain LBA 9402 (pRil855). Transformed plants were uniform and had a distinct phenotype and development compared with untransformed controls. Their growth was vigorous, especially early in their development, their roots were abundant and showed reduced geotropism, their leaves were slightly crinkled and glossy and they produced longer tubers with more frequent, prominent eyes. Cytological examination showed that ten of the forty-two transformed plants had either 47 or 49 chromosomes instead of the normal 48. In two of these aneuploids structural changes were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273752
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 744-750 
    Details
    ISSN: 1432-2242
    Keywords: Genetic manipulation ; Neomycin phosphotransferase ; Mixed infection ; Somaclonal variation ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Derivatives of potato (Solanum tuberosum cv.'s ‘Maris Bard’ and ‘Desiree’) transformed with disarmed T-DNA from genetically engineered Agrobacterium tumefaciens strains were isolated. The transformed plants were recovered from shoot-forming tumours induced by infection of wounds with mixedcultures of shoot-inducing A. tumefaciens strains T37 and either Agrobacterium strain LBA1834(pRAL1834), (Hille et al. 1983) or LBA4404(pBIN6; pRAL4404), (Bevan 1984). Two small-scale feasibility experiments gave at least four ‘Maris Bard’ plants transformed with pRAL1834 T-DNA and two ‘Desiree’ plants with pBIN6 T-DNA. The transformed ‘Maris Bard’ plants were morphologically abnormal and highly aneuploid. This was probably an unfortunate side-effect of a tissue culture-step introduced to promote the efficiency of shoot regeneration. The transformed ‘Desiree’ plants, in contrast, were isolated without promoting additional shoot-growth. They were morphologically normal, contained 47 and the euploid 48 chromosomes per cell respectively and had improved growth on media containing kanamycin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260785
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  • 3
    Electronic Resource
    Electronic Resource
    Presence of Locusta diuretic hormone in endocrine cells of the ampullae of locust Malpighian tubules (1996)
    Springer
    Cell & tissue research 285 (1996), S. 331-339 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Diuretic hormone ; Endocrine cells ; Midgut ; Malpighian tubules ; Bioassay ; Immunocytochemistry ; Locusta migratoria (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. This is an investigation of an endocrine cell type in the midgut of the migratory locust Locusta migratoria. This cell type is found in the posterior region of the midgut and is especially common in the ampullae through which Malpighian tubules drain into the gut at the midgut-hindgut junction. Strong Locusta diuretic hormone-like immunoreactivity in these cells was colocalized with FMRFamide- and substance P-like immunoreactivities. At the ultrastructural level, immunoreactivity for Locusta diuretic hormone was found in spherical granules (mean diameter of 450 nm), the contents of which showed variable electron density. Fractionation of a methanolic extract of the ampullae by reversed-phase high performance liquid chromatography revealed the presence of two peaks of Locusta diuretic hormone-like immunoreactive material, both of which stimulate cyclic AMP production by isolated Malpighian tubules. The more hydrophobic material is most likely Locusta diuretic hormone, which has the same retention time when chromatographed under identical conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050650
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  • 4
    Electronic Resource
    Electronic Resource
    Developmental regulation of RI TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree) (1986)
    Springer
    Plant molecular biology 6 (1986), S. 321-330 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium rhizogenes ; Solanum tuberosum ; T-DNA ; gene expression ; tuberisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Expression of TL-DNA from Agrobacterium rhizogenes plasmid pRi 1855 was examined in a transformed derivative of Solanum tuberosum cv. Desiree, D9X8a. Northern blot analysis identified at least nine TL-DNA coded transcripts in roots, shoots and tubers but their relative abundance differed within and between organs. This revealed a distinctive pattern of organ specified differential expression. Grafting experiments showed that the abnormal shape of tubers of transformed potato was probably determined by TL-DNA products synthesised within the tuber and not by diffusable products synthesised in other parts of the plant. The abundance of at least one transcript, tr5, was probably determined by culture conditions. Implications for functions and control of expression of Ri TL-DNA genes are discussed. It is suggested that Ri TL-DNA provides a convenient and extensive set of model genes to study variation and stability of expression of linked foreign genes introduced into plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034939
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