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  • Life and Medical Sciences  (7)
  • Senescence  (4)
  • Wiley-Blackwell  (7)
  • Springer  (4)
  • American Institute of Physics
  • American Institute of Physics (AIP)
  • Blackwell Publishing Ltd
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Keywords
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  • Wiley-Blackwell  (7)
  • Springer  (4)
  • American Institute of Physics
  • American Institute of Physics (AIP)
  • Blackwell Publishing Ltd
Years
  • 1
    Electronic Resource
    Electronic Resource
    Lariat RNA of a group II intron in a filamentous fungus (1987)
    Springer
    Current genetics 12 (1987), S. 291-295 
    Details
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Group II intron ; Lariat RNA ; Senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In senescing strains of the filamentous ascomycete Podospora anserina, the first intron (il) of the mitochondrial gene for cytochrome-c-oxidase subunit I (CO I), a group II intron, accumulates as a circular mitochondrial plasmid (plDNA). In both juvenile and senescent wild type strain s two highly abundant transcripts were detected homologous to il and plDNA. In this report we show that these RNAs are identical molecules having different conformations: the first is a lariat, the second a corresponding linear molecule probably resulting from breakage of the branched circular form. Our findings suggest that the transcripts arise from processing of CO I pre-mRNA, including il, rather than from transcription of the excised plasmid. The significance of the lariat RNA concerning plDNA amplification via a postulated reverse transcription mechanism is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435292
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  • 2
    Electronic Resource
    Electronic Resource
    Transformation to senescence with plasmid like DNA in the ascomycete Podospora anserina (1980)
    Springer
    Current genetics 2 (1980), S. 181-184 
    Details
    ISSN: 1432-0983
    Keywords: Transformation ; Senescence ; Podospora anserina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the ascomycete Podospora anserina senescence through strain aging is under nucleo-cytoplasmic control and inducible in juvenile mycelia by an ‘infective principle’ transferred after cytoplasmic contact via anastomoses. A specific DNA called plasmid-like (pl) DNA, present exclusively in aging mycelia, was found to be identical with this ‘infective principle’, since it was possible to transform juvenile protoplasts to senescence by using purified p1DNA. Therefore a specific function may be attributed to this ccc DNA. Its direct involvement in a genetically programed senescence is confirmed and its development as a vector for transfer of genetic information in eukaryotes can be undertaken.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435683
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  • 3
    Electronic Resource
    Electronic Resource
    Development of a eukaryotic cloning system in Podospora anserina (1982)
    Springer
    Current genetics 6 (1982), S. 219-222 
    Details
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Eukaryotic cloning system ; Senescence ; Long-lived mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In developing a system for molecular cloning with the Podospora anserina plasmid (p1DNA) it is necessary to find recipient strains which are resistant to p1DNA mediated senescence. Three long lived double mutants which fail to exhibit spontaneous aging were genetically and biochemically analysed. All mutants were infected with p1DNA. The mutant ca viv became irreversibly senescent and therefore was not further tested. The second mutant, gr viv showed some symptoms of aging but never died. The third strain i viv remained resistant to aging from p1DNA infection and has thus proven to be the best host strain available for molecular cloning in this system. A DNA analysis of the latter two strains revealed: 1. There is no difference from the wild strain with respect to the structure of mtDNA and the integration site of the p1DNA. 2. Of the two strains, only i viv contains free p1DNA in its mitochondria but in low amounts if compared to the wild strain. These experimental results are interpreted as follows: 1. The gr viv strain does not liberate spontaneously the p1DNA from mtDNA, but following infection is able to replicate and express this plasmid and therefore is a potential host for transformation. 2. The i viv strain liberates the mitochondrial plasmid but does not express senescence even when infected with p1DNA. Therefore, this strain is an ideal recipient for transformation provided a marker other than senescence is cloned.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390341
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  • 4
    Electronic Resource
    Electronic Resource
    The onset of senescence is affected by DNA rearrangements of a discontinuous mitochondrial gene in Podospora anserina (1985)
    Springer
    Current genetics 9 (1985), S. 373-382 
    Details
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Rearrangements of mtDNA ; Senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mapping and transcription studies have revealed that in Podospora anserina the causative agent of senescence, a mitochondrial plasmid (p1DNA), is identical with intronl of the discontinuous gene for cytochrome-c-oxidase subunit 1 (COI), which is 2 kpb from the discontinuous gene for cytochrome b (Cytb). A mitochondrial mutant (ex1) devoid of the COI, but not of the Cytb gene provides longevity. A molecular model for the onset of senescence is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421608
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  • 5
    Electronic Resource
    Electronic Resource
    Development and growth of compound tooth plates in Callorhinchus milii (chondrichthyes, holocephali) (1994)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 222 (1994), S. 73-89 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The chimaeroid holocephalian fishes are distinguished among extant chondrichthyans by the possession of three pairs of tooth plates, evergrowing and partially hypermineralized, that are not shed and replaced like the teeth of living elasmobranchs. Although derivation of the chimaeroid tooth plate from the fusion of members of a plesiomorphic chondrichthyan tooth family has been proposed, evidence for this hypothesis has been lacking. A new analysis of the development and structure of the tooth plates in Callorhinchus milii (Holocephali, Chimaeriformes) reveals the compound nature of the tooth plates in a chimaeroid fish. Each tooth plate consists of an oral and aboral territory that form independently in the embryo and maintain separate growth surfaces through life. The descending lamina on the aboral surface of the tooth plate demarcates the growth surface of the aboral territory. Comparison with the tooth plates of Chimaera monstrosa indicates that compound tooth plates may be a feature of all chimaeroids in which a descending lamina is present. The tooth plates in these fishes represent the fusion of two members of a reduced tooth family. The condition of the tooth plates in C. milii is plesiomorphic for chimaeroids and is of evolutionary significance in that it provides further evidence to support a lyodont dentition in chimaeroid fishes similar to that found in other chondrichthyans. © 1994 Wiley-Liss, Inc.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052220108
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  • 6
    Electronic Resource
    Electronic Resource
    Circular genetic maps (1967)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 70 (1967), S. 1-12 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The widespread occurrence of genetic circularity suggests a selective advantage to map circularity per se. Circularity permits gene clustering relations not possible in linear maps; that is, every gene in a circular map can have two nearest map neighbors, two next nearest, etc. It seems possible that map circularity is a consequence of the selective forces responsible for the clustering of genes of related function. Certain features of the pattern of crossing-over in various fungi suggest that circularity of linkage maps is there to be found. A concurrence of high frequencies of second-division segregation and negative chromatid interference across the centromere, both of which phenomena have been reported, is a feature of a simple hypothetical crossover pattern that generates circular linkage maps.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040700403
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  • 7
    Electronic Resource
    Electronic Resource
    Genetic recombination and replication in bacteriophageMost of the new experiments described in this paper were performed by the first two authors at the Biology Division of the Oak Ridge National Laboratory under Contract NO. W-7405-eng-26 for the Atomic Energy Commission.The remainder of the recent experiments and most of the analyses were completed at the University of Rochester and supported by a research grant, C-2306, from the National Cancer Institute of the National Isntitutes of Health, Public Health Service. (1955)
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 45 (1955), S. 51-74 
    Details
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1030450505
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  • 8
    Electronic Resource
    Electronic Resource
    Das Experiment: Dikaryophase und Bildung von Meiosporangien bei höheren Pilzen (1976)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 6 (1976), S. 53-57 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.19760060205
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  • 9
    Electronic Resource
    Electronic Resource
    Development and tissue-specific distribution of mouse small heat shock protein hsp25 (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 103-111 
    Details
    ISSN: 0192-253X
    Keywords: Mouse ; development ; small heat shock protein ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have investigated the developmental and tissue-specific distribution of the mouse small hsp25 by immunohistology using an antibody that specifically identifies hsp25. Our analysis shows that the relative amount of hsp25 increases during embryogenesis. Through days 13-20 of embryogenesis, hsp25 accumulation is predominant in the various muscle tissues, including the heart, the bladder, and the back muscles. hsp25 is detectable also in neurons of the spinal cord and the purkinje cells. Furthermore analysis of the closely related α, B-crystallin shows that in several tissues, including the bladder, the notochordal sheath and the eye lens both proteins are coexpressed. Our studies demonstrate that mammalian hsp25 accumulation is developmentally regulated during mouse embryogenesis and support the view of an important functional role of small heat shock proteins in normal cell metabolism. © 1993Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020140204
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  • 10
    Electronic Resource
    Electronic Resource
    Influence of the codon following the initiation codon on the expression of the lacZ gene in Saccharomyces cerevisiae (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 7 (1991), S. 157-165 
    Details
    ISSN: 0749-503X
    Keywords: Translation initiation ; codon usage: mRNA structure ; yeast ; lacZ fusion protein ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A set of 32 different codons were introduced in a lacZ experssion vector (pPTK400) immediately 3′ from the AUG initiation codon. Expression of the lacZ gene was determined in Saccharomyces cerevisiae by measuring the amount of β-galactosidase fusion protein using immuno-gel electrophoresis. A 5·3-fold difference in expression was found among the various constructs. It was found that there was no preference for a certain nucleotide in any position of the second codon and there was no distinct correlation between the level of tRNA corresponding to any particular second codon and expression. No correlation could be found between the local secondary structure and expression. When the overall codon usage in yeast and the codon usage in the second position of the mRNA is compared, there is no obvious significant difference in preference. This indicates that in yeast, in contrast to Escherichia coli, the codon choice at the beginning of the mRNA does not deviate from the one further downstream and is determined by the requirements for optimal translation elongation. Important determinatnts of the optimal context for an initiation codon in yeast therfore must be located mainly 5′ from this codon.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320070209
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