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  • cDNA  (9)
  • Korea  (7)
  • permeability  (7)
  • thermal diffusivity  (7)
  • sexual isolation  (5)
  • Springer  (35)
  • American Geophysical Union (AGU)
  • 1
    Electronic Resource
    Electronic Resource
    Prevalence of human foamy virus-related sequences in the Korean population (1998)
    Springer
    Journal of biomedical science 5 (1998), S. 267-273 
    Details
    ISSN: 1423-0127
    Keywords: Grave's disease ; Human foamy virus ; Korea ; Molecular epidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The possible association of human foamy virus (HFV) with human thyroid disorders such as Graves' disease (GD) has been a topic of controversy due to the inconsistent results reported by several groups of investigators. Here we report the investigation of the presence of HFV-related sequences in the Korean population. DNA was obtained from peripheral blood lymphocytes from 24 GD patients and 23 healthy blood donors and subjected to PCR amplification using three sets of nested primers derived fromgag, env, and LTR regions of the HFV genome. Contrary to previously reported studies, our analysis identified HFV-related sequences in the genomes of both healthy individuals and the GD patients. However, the nature of the HFV genome present in each group appeared to be different. We detected all 3 regions of HFV-related sequences in 29% of the HFV-positive GD patients, while no samples in the control group amplified all three regions. This suggests that the LTR may be used as a tool for screening for HFV in GD patients. Our data favor the hypothesis of a relationship between GD and the presence of HFV-related sequences, though in a complex way.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02255858
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning and characterization of anther-preferential cDNA encoding a putative actin-depolymerizing factor (1993)
    Springer
    Plant molecular biology 21 (1993), S. 39-45 
    Details
    ISSN: 1573-5028
    Keywords: actin-depolymerizing factor ; cDNA ; developmental regulation ; male gametophyte ; anther
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone, LMP131A, which is preferentially expressed in mature anther was isolated from a lily cDNA library. Northern blot analysis and plaque hybridization expriments showed that the LMP131A mRNA is present at ca. 0.3% of the mRNA in mature pollen and is not detectable in carpel, petal, floral bud, leaf, or root. The clone contains an open reading frame of 139 amino acid residues which shows greater than 40% sequence identity in a 91 amino acid overlap to animal actin-depolymerizing factors (ADF), cofilin and destrin. The sequences at and near the actin-binding site are most conserved. Using the lily clone as a probe, a cDNA clone, BMP1, was isolated from a mature anther library of Brassica napus. The expression pattern of the BMP1 clone was the same as that of the lily clone. The Brassica anther-preferential clone contains an open reading frame which is 79% identical to the lily LMP131A protein. Southern blot analysis showed that there are one or a few copies of the putative ADF genes in B. napus and Arabidopsis thaliana.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039616
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  • 3
    Electronic Resource
    Electronic Resource
    Evaluation of a Human Bio-Engineered Skin Equivalent for Drug Permeation Studies (2000)
    Springer
    Pharmaceutical research 17 (2000), S. 1092-1097 
    Details
    ISSN: 1573-904X
    Keywords: drug delivery systems ; skin alternatives ; transdermal drug delivery ; permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. To test the barrier function of a bio-engineered human skin (BHS) using three model drugs (caffeine, hydrocortisone, and tamoxifen) in vitro. To investigate the lipid composition and microscopic structure of the BHS. Methods. The human skin substitute was composed of both epidermal and dermal layers, the latter having a bovine collagen matrix. The permeability of the BHS to three model drugs was compared to that obtained in other percutaneous testing models (human cadaver skin, hairless mouse skin, and EpiDerm™). Lipid analysis of the BHS was performed by high performance thin layered chromatrography. Histological evalulation of the BHS was performed using routine H&E staining. Results. The BHS mimicked human skin in terms of lipid composition, gross ultrastructure, and the formation of a stratum corneum. However, the permeability of the BHS to caffeine, hydrocortisone, and tamoxifen was 3-4 fold higher than that of human cadaver skin. Conclusions. In summary, the results indicate that the BHS may be an acceptable in vitro model for drug permeability testing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026405712870
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  • 4
    Electronic Resource
    Electronic Resource
    Thermal Diffusivity Measurement of Two-Layer Optical Thin-Film Systems Using Photoacoustic Effect1 (2000)
    Springer
    International journal of thermophysics 21 (2000), S. 543-552 
    Details
    ISSN: 1572-9567
    Keywords: antireflection coating ; parameter estimation ; photoacoustic effect ; thermal diffusivity ; through-plane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In this study, we designed and developed two-layer antireflection (AR) optical coating samples on glass substrates, using different evaporation conditions of coating rates and substrate temperatures for two dielectric materials, MgF2 and ZnS, with different refractive indices. The through-plane thermal diffusivity of these systems was measured using the photoacoustic effect. The optical thicknesses of MgF2 and ZnS layers were fixed at 5λ/4 (λ=514.5 nm) and λ, respectively, and the thermal diffusivities of the samples were obtained from the measured amplitude of the photoacoustic signals by changing the chopping frequency of the Ar+ laser beam. The results demonstrated that the thermal diffusivity of the sample fabricated under the conditions of 10μÅ·s−1 and 150°C had the maximum value and that the results were directly related to the microstructure of the film system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006612403028
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  • 5
    Electronic Resource
    Electronic Resource
    Metal contamination of soils and dusts in Seoul metropolitan city, Korea (1995)
    Springer
    Environmental geochemistry and health 17 (1995), S. 139-146 
    Details
    ISSN: 1573-2983
    Keywords: Metal contamination ; Seoul ; Korea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Medicine
    Notes: Abstract To investigate the dispersion patterns and the characteristics of heavy metal contamination due to urbanisation and industrialisation, soils and dusts collected from the Seoul area were analysed for Cu, Pb, Zn and Cd. The metal concentrations in most soils and dusts are higher than the world averages. The pollution index ((Σ Metal concentrations in soils/Permissible level for metal)÷(Number of metals)) of soils and dusts is 〉 1 in most of the Seoul area, a result that concurs with the industrialisation and urbanisation index of the Seoul area. The soils are contaminated with Cu, Zn, Cd and particularly Pb. This suggests that the contamination of the soils in the Seoul area are mainly caused by vehicular emissions. The pollution index of soil is the highest in the Kuro area where Cu and Zn contamination in soils are due to the indigenous brass and bronze factories. From the discriminant analysis, the Seoul area may be partitioned into control, traffic and industrialized areas by the metal concentrations in the order of Zn 〉 Cu 〉 Pb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00126082
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  • 6
    Electronic Resource
    Electronic Resource
    A new model for impregnation mechanisms in different GF/PP commingled yarns (1994)
    Springer
    Applied composite materials 1 (1994), S. 351-371 
    Details
    ISSN: 1573-4897
    Keywords: commingled yarn ; impregnation ; consolidation ; thermoplastics ; processing ; permeability ; mechanical testing ; mathematical modelling ; compression moulding ; glass fibre bed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Impregnation mechanisms of different kinds of GF/PP commingled yarns have been studied. As the reinforcing fibres were always the same, a global description has been worked out. Two different mathematical approaches for fibre bed permeability (Kozeny-Carman and Gutowski) were compared. The constants of the applied mathematical models have to stay the same if the fibre reeinforcement and the fibre arrangement is the same. Neither the kind of matrix, nor the fibre volume content may change these constants. Differences in the degree of impregnation after the same process conditions can be only due to different sizes of fibre agglomerations, thus the initial distribution of reinforcing fibres and matrix. For an exact determination of impregnation times and conditions the exact distribution of fibres in the intermediate material and after processing has to be known. This distribution is determined by SEM microscopy and data given from the material supplier. The importance of different process parameters, such as temperature, pressure, processing time is weighted by determining the density and mechanical properties of the specimens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00568041
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  • 7
    Electronic Resource
    Electronic Resource
    Developmental isolation and subsequent adult behavior ofDrosophila paulistorum. II. Prior experience (1996)
    Springer
    Behavior genetics 26 (1996), S. 15-25 
    Details
    ISSN: 1573-3297
    Keywords: Mating behavior ; reproductive isolation ; sexual isolation ; semispecies ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Preadult seclusion imposed upon members of theDrosophila paulistorum complex of intersterile semispecies significantly decreased sexual isolation between adults of the semispecies. While seclusion at any developmental stage had this effect, there were quantitative variations correlated with the stage at which seclusion had been initiated: the earlier the stage at which seclusion began, the more frequent were the heterogamic matings. All the stages of development seemed to contribute to final adult sexual behavior, with no single stage emerging as the most important experiential phase. Seclusion also significantly affected intrasemispecific matings, in that flies were more likely to mate with partners having similar experiences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02361155
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  • 8
    Electronic Resource
    Electronic Resource
    Developmental isolation and subsequent adult behavior ofDrosophila paulistorum. I. Survey of the six semispecies (1992)
    Springer
    Behavior genetics 22 (1992), S. 545-556 
    Details
    ISSN: 1573-3297
    Keywords: mating behavior ; reproductive isolation ; sexual isolation ; sexual selection ; semispecies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract We present results deriving from the inception of an investigation (involving 4,000 scored matings) into the development of discriminatory behavior inDrosophila paulistorum. We examined two complementary situations for their effects on mating behavior: physical isolation (seclusion) beginning at each of six developmental stages and exposure to heterosemispecifics during all preadult stages (mixed culturing). Preadult seclusion at certain stages decreased sexual isolation between three of four semispecific pairs. Seclusion effect, or lack thereof, varied with semispecies and with the stage at which seclusion was imposed: the earlier the stage at which seclusion was initiated, the more drastic the effect. In contrast, mixed culturing increased sexual isolation between the one pair of semispecies that was tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01074307
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  • 9
    Electronic Resource
    Electronic Resource
    Developmental isolation and subsequent adult behavior ofDrosophila paulistorum. III. Alternative rearing (1996)
    Springer
    Behavior genetics 26 (1996), S. 27-37 
    Details
    ISSN: 1573-3297
    Keywords: Mating behavior ; reproductive isolation ; sexual isolation ; semispecies ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract We have been utilizing membres of theDrosophila paulistorum complex of semispecies to study the development of adult discriminatory behavior. In this study, three rearing methods were used to examine the effects of previous exposure to heterosemispecifics on reproductive isolation among the various semispecies. Experimental flies were exposed to heterosemispecifics by one of three methods: exposure to airborne heterosemispecific stimuli from egg through sexual maturity, physical contact with heterosemispecifics only during the postimaginal period, and mixed culturing (total physical contact with heterosemispecific individuals during each of five preimaginal stages). Effects of the first treatment varied with semispecies. The second treatment had no significant effect on mating behavior. The third treatment of mixed culturing reinforced sexual isolation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02361156
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  • 10
    Electronic Resource
    Electronic Resource
    A rice (Oryza sativa L.) cDNA encodes a protein sequence homologous to the eukaryotic ribosomal 5S RNA-binding protein (1993)
    Springer
    Plant molecular biology 23 (1993), S. 409-413 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; cloning ; rice ; L5 ; ribosomal 5 S RNA-binding protein ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rice (Oryza sativa L.) cDNA clone coding for the cytoplasmic ribosomal protein L5, which associates with 5 S rRNA for ribosome assembly, was cloned and its nucleotide sequence was determined. The primary structure of rice L5, deduced from the nucleotide sequence, contains 294 amino acids and has intriguing features some of which are also conserved in other eucaryotic homologues. These include: four clusters of basic amino acids, one of which may serve as a nucleolar localization signal; three repeated amino acid sequences; the conservation of glycine residues. This protein was identified as the nuclear-encoded cytoplasmic ribosomal protein L5 of rice by sequence similarity to other eucaryotic ribosomal 5 S RNA-binding proteins of rat, chicken, Xenopus laevis, and Saccharomyces cerevisiae. Rice L5 shares 51 to 62% amino acid sequence identity with the homologues. A group of ribosomal proteins from archaebacteria including Methanococcus vanniellii L18 and Halobacterium cutirubrum L13, which are known to be associated with 5 S rRNA, also related to rice L5 and the other eucaryotic counterparts, suggesting an evolutionary relationship in these ribosomal 5 S RNA-binding proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029016
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