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  • Fluorescence detection  (4)
  • Springer  (4)
  • American Geophysical Union (AGU)
Collection
Keywords
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  • Springer  (4)
  • American Geophysical Union (AGU)
Years
  • 1
    Electronic Resource
    Electronic Resource
    Analysis and chromatographic separation of some steroid hormones on NH2 layers (1994)
    Springer
    Chromatographia 39 (1994), S. 97-102 
    Details
    ISSN: 1612-1112
    Keywords: Thin-layer chromatography ; Amino modified silica gel ; Steroid hormones ; Thermal activation ; Fluorescence detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The previously described analytical method for carbohydrates, catecholamines, uric acid, creatine and creatinine using thin-layer chromatography on aminomodified HPTLC plates and subsequent thermal activation of the chromatogram zones is expanded to include several steroid hormones. Specifically, they are the pharmacologically relevant compounds cortisone and hydrocortisone, estradiol and estradiol benzoate, estriol, estrone, methyltestosterone, testosterone and testosterone propionate, prednisolone, pregnandiol and triol, progesterone and Reichstein's S.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02320466
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Use of a new adsorbent in the separation and detection of glucose and fructose by HPTLC (1989)
    Springer
    Chromatographia 28 (1989), S. 364-366 
    Details
    ISSN: 1612-1112
    Keywords: Thin-layer chromatography ; HPTLC ; Glucose and fructose ; Fluorescence detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simple method for separating and detecting glucose and fructose is described. Merely heating the chromatogram under an IR radiator, or on a hotplate or in a drying cabinet, renders components separated on HPTLC plates NH2 visible under UV light. The detection limits are similar to those obtained with spray reagents and dipping techniques. Possible application of the method to other carbohydrates is briefly mentioned.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02261015
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  • 3
    Electronic Resource
    Electronic Resource
    Application of a thermalin situ reaction for fluorimetric detection of carbohydrates on NH2-layers (1990)
    Springer
    Chromatographia 29 (1990), S. 467-472 
    Details
    ISSN: 1612-1112
    Keywords: Thin-layer chromatography ; NH2-layers ; Carbohydrates ; Fluorescence detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Based on experience in the purely thermally induced (no analytical reagents) fluorimetric detection of glucose and fructose after thin-layer chromatographic separation on NH2-layers, the application of this method to other sugars is described. After separation with mobile phases containing pyridine, this “dry”in situ derivatization permits a very simple qualitative and quantitative method of detection for the 20 carbohydrates studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02261396
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  • 4
    Electronic Resource
    Electronic Resource
    Qualitative and quantitative analysis of uric acid, creatine and creatinine together with carbohydrates in biological material by HPTLC (1991)
    Springer
    Chromatographia 32 (1991), S. 307-316 
    Details
    ISSN: 1612-1112
    Keywords: Thin-layer chromatography ; Amino modified silica ; Creatine, creatinine, uric acid and carbohydrates ; Fluorescence detection ; In-situ densitometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary In clinical diagnosis creatine, creatinine and uric acid are important parameters for the evaluation of renal diseases, and are partially responsible for gout and the formation of renal calculi. The determination of various carbohydrates, especially glucose, in urine and serum serves as an indicator of diabetes and other carbohydrate anomalies. A simple, rapid and economic method for the simultaneous screening and quantitation of these compounds is presented. No sample preparation is necessary for the determination in urine or in serum. The proposed method consists of separations on an amino modified HPTLC precoated plate. The detection of all relevant substances is reproducibly performed by simply heating the chromatogram to give stable fluorescent derivatives. The detection limits in all cases lie significantly below the physiological range.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02321427
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