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  • Drosophila melanogaster  (8)
  • Korea  (7)
  • permeability  (7)
  • Arabidopsis thaliana  (5)
  • Springer  (27)
  • American Geophysical Union (AGU)
  • 11
    Electronic Resource
    Electronic Resource
    Cereport™ (RMP-7) Increases the Permeability of Human Brain Microvascular Endothelial Cell Monolayers1 (1999)
    Springer
    Pharmaceutical research 16 (1999), S. 1360-1365 
    Details
    ISSN: 1573-904X
    Keywords: Cereport ; bradykinin B2 receptor ; human ; brain endothelium ; permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. To study Cereport (RMP-7, bradykinin B2 agonist) effects on human brain microvascular endothelial cell (HBMEC) monolayer permeability. Methods. HBMEC grown on transwell membranes were exposed to Cereport. The monolayer permeability was determined with [I4C]-inulin (MW. 5,200) and [3H]-dextran (MW. 70,000). Results. Cereport increased the HBMEC permeability to [l4C]-inulin, but not to [3H]-dextran. The effect was transient, maximal at 15 min (i.e., 79.3% increase), and polarized to the basolateral membrane. An inverted U, dose-response curve was observed with active concentrations of Cereport from 0.01 to 0.5 nmol/L, the plateau maximal effect between 0.5 and 10 nmol/L, and loss of activity at the highest concentration, i.e., 20 nmol/L. Cyclic AMP-specific phosphodiesterase 3 (PDE3) inhibitor rolipram (10 μmol/L) abolished Cereport effects, while cGMP-specific PDE5 inhibitor, zaniprast (50 μmol/L) enhanced by 31 % (p 〈 0.05) the effect of 0.1 nmol/L Cereport. Unlabeled Cereport displaced [12 5I]-bradykinin and/or [125I]-Cereport from the basolateral side. There was no specific Cereport binding to the apical side. Conclusions. Cereport exerts specific time, dose and size dependent actions on HMBEC monolayer that are restricted to the basolateral membrane. Its effects can be further modulated through changes in cAMP and cGMP second messenger systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018938722768
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  • 12
    Electronic Resource
    Electronic Resource
    Autonomous transposition of gypsy mobile elements and genetic instability in Drosopbila melanogaster (1990)
    Springer
    Molecular genetics and genomics 224 (1990), S. 303-308 
    Details
    ISSN: 1617-4623
    Keywords: Transposable elements ; Genetic instability ; Drosophila melanogaster ; gypsy (mdg4)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The laboratory imitator strain (MS) of Drosophila melanogaster is characterized by an elevated frequency of spontaneous mutation (10−3–10−4). Mutations occur in both sexes at premeiotic stages of germ cell development. The increased mutability is a characteristic feature of MS itself, since it appears in the absence of outcrossing. Most of the mutations arising in this strain are unstable: reversions to wild type, high frequency mutation to new mutant states and replicating instability were observed. We have investigated the localization of the transposable genetic elements mdg1, 412, mdg3, gypsy (mdg4), copia and P in the X chromosomes of the MS and in the mutant lines y, ct, sbt derived from it by in situ hybridization. The P element was not found in any of these strains. The distributions of mdg1, 412, mdg3 and copia were identical in the X chromosomes of the MS and its derivatives. However, the sites of hybridization with gypsy differ in the various lines tested. In the polytene chromosomes of MS animals significant variation in location and number of copies of the gypsy element was demonstrated between different larvae; copy numbers as high as 30–40 were observed. These results suggest autonomous transposition of gypsy in the MS genome while several other mobile elements remain stable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271566
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  • 13
    Electronic Resource
    Electronic Resource
    The introduction of a transpositionally active copy of retrotransposon GYPSY into the Stable Strain of Drosophila melanogaster causes genetic instability (1994)
    Springer
    Molecular genetics and genomics 242 (1994), S. 472-477 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila melanogaster ; Genetic instability ; gypsy ; Mobile elements ; Retrotransposons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A previously described genetic system comprising a Mutator Strain (MS) and the Stable Strain (SS) from which it originated is characterized by genetic instability caused by transpositions of the retrotransposon gypsy. A series of genetic crosses was used to obtain three MS derivatives, each containing one MS chromosome (X, 2 or 3) in the environment of SS chromosomes. All derivatives are characterized by elevated frequencies of spontaneous mutations in both sexes. Mutations appear at the premeiotic stage and are unstable. Transformed derivatives of SS and another stable strain 208 were obtained by microinjection of plasmid DNA containing transpositionally active gypsy inserted into the Casper vector. In situ hybridization experiments revealed amplification and active transposition of gypsy in SS derivatives, while the integration of a single copy of gypsy into the genome of 208 does not change the genetic properties of this strain. We propose that genetic instability in the MS system is caused by the combination of two factors: mutation(s) in gene(s) regulating gypsy transposition in SS and its MS derivatives, and the presence of transpositionally active gypsy copies in MS but not SS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281799
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  • 14
    Electronic Resource
    Electronic Resource
    Transposition of mobile elements gypsy (mdg4) and hobo in germ-line and somatic cells of a genetically unstable mutator strain of Drosophila melanogaster (1991)
    Springer
    Molecular genetics and genomics 229 (1991), S. 437-444 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila melanogaster ; Transposition ; Genetic instability ; gypsy (mdg4) ; hobo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using the in situ hybridization technique, we have analysed the distribution of mobile elements in the X chromosomes of male offspring of individual mutator strain (MS) males crossed to attached-X females. The experiments demonstrate varying cytological localization of the mobile elements gypsy (mdg4) and hobo among different individuals. The other mobile elements investigated (mdgl, mdg3, 412, 297, copia, 17.6, Doc, H.M.S. Beagle, Springer, FB) display no changes in insertion sites. Such an experiment is equivalent to analysis of separate gametes of an MS individual. Thus, the ability of gypsy and hobo to transpose in germ-line cells is demonstrated directly. Transpositions occur at premeiotic stages of germ cell development, since they appear in clusters. Analysis of gypsy and hobo transposition events shows that they occur independently. The same experiment demonstrates that gypsy localization varies significantly between different salivary gland cells of an MS individual. Two types of gypsy hybridization sites can be distinguished: “permanent” sites, common to all cells, and “additional” ones varying between neighbouring salivary gland cells. These additional sites indicate gypsy transposition in somatic cells of the MS. Transposition of the hobo element in somatic cells has also been observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267467
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  • 15
    Electronic Resource
    Electronic Resource
    Molecular analysis of the gypsy (mdg4) retrotransposon in two Drosophila melanogaster strains differing by genetic instability (1990)
    Springer
    Molecular genetics and genomics 223 (1990), S. 305-309 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila melanogaster ; Genetic instability ; gypsy (mdg4) ; Retrotransposon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The structural organization of the retrotransposon gypsy (mdg4) is investigated in two Drosophila melanogaster strains. One of them, the stable w strain (SS), is characterized by a small copy number and stable localization of gypsy. In the other, unstable mutator strain (MS) which is derived from SS, the gypsy copy number and the frequency of its transposition are greatly increased. Genomic gypsy copies cloned from both strains display structural differences allowing them to be divided into two subfamilies. At the nucleotide level, these differences involve single substitutions, deletions and insertions. Southern blot analysis revealed that SS possesses only gypsy elements that belong to one subfamily, while in MS only gypsy copies from the other subfamily were amplified and transposed. The transcriptional activity of gypsy was also studied. Despite the structural differences, plasmid-borne copies of each type of gypsy exhibit equal transcriptional activity in transfected tissue culture cells. Nevertheless, although a high level of gypsy transcription is observed in MS, gypsy poly(A)+RNA is not detected in SS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265067
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  • 16
    Electronic Resource
    Electronic Resource
    COP1b, an isoform of COP1 generated by alternative splicing, has a negative effect on COP1 function in regulating light-dependent seedling development in Arabidopsis (1998)
    Springer
    Molecular genetics and genomics 257 (1998), S. 387-391 
    Details
    ISSN: 1617-4623
    Keywords: Key words Photomorphogenesis ; COP1 ; Alternative splicing ; Seedling development ; Arabidopsis thaliana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract COP1 is a negative regulator of Arabidopsis light-dependent development. Mutation of the COP1 locus causes constitutive photomorphogenesis in the dark. Here, we report the identification of an isoform of the COP1 protein, named COP1b, which is generated by alternative splicing. COP1b has a 60-amino acid deletion in the WD-40 repeat domain relative to the full-length COP1. This splicing step is light-independent and takes place mostly in mature seeds and in germinating seedlings. Transgenic Arabidopsis plants that overexpress COP1b show a de-etiolated phenotype in the dark, with a short hypocotyl, open and developed cotyledons. The transgenic seedlings are adult-lethal. These phenotypes closely resemble that of severe cop-1 mutants, indicating that COP1b has a dominant negative effect on COP1 function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050662
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  • 17
    Electronic Resource
    Electronic Resource
    Characterisation of the PsbX protein from Photosystem II and light regulation of its gene expression in higher plants (1999)
    Springer
    Plant molecular biology 40 (1999), S. 737-744 
    Details
    ISSN: 1573-5028
    Keywords: 4.1 kDa protein ; Arabidopsis thaliana ; low-molecular-mass proteins ; photosystem II ; PsbX ; spinach
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The location and expression of the previously uncharacterised photosystem II subunit PsbX have been analysed in higher plants. We show that this protein is a component of photosystem II (PSII) core particles but absent from light-harvesting complexes or PSII reaction centres. PsbX is, however, localised to the near vicinity of the reaction centre because it can be cross-linked to cytochrome b559, which is known to be associated with the D1/D2 dimer. We also show that the expression of this protein is tightly regulated by light, since neither protein nor mRNA is found in dark-grown plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006286706708
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  • 18
    Electronic Resource
    Electronic Resource
    Bdelloid rotifers from Korea (2000)
    Springer
    Hydrobiologia 439 (2000), S. 91-101 
    Details
    ISSN: 1573-5117
    Keywords: Rotifera ; Bdelloidea ; Korea ; taxonomy ; biogeography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-five samples from 18 terrestrial and/or freshwater habitats in the eastern part of Korea yielded 38 new records of bdelloid rotifers, 22 of which are new to the Asian fauna. Among these Korean new records, Dissotrocha aculeata reversa Berzins and Habrotrocha plana Milne were recorded outside their type localities for the first time, and Macrotrachela bullata (Murray) was reported only from two countries after its description. Bradyscela granulosa de Koning, Habrotrocha gracilis gracilis Montet, Macrotrachela latior Donner, Philodina duplicalcar (de Koning) and P. rugosa coriacea Bryce are recorded outside Europe for the first time. The taxonomy and distribution of these rare species are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004181414535
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  • 19
    Electronic Resource
    Electronic Resource
    An Arabidopsis thaliana protein homologous to cyanobacterial high-light-inducible proteins (2000)
    Springer
    Plant molecular biology 42 (2000), S. 345-351 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; ELIP ; EST ; high light ; HLIP ; LHC protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An Arabidopsis thaliana cDNA clone encoding a novel 110 amino acid thylakoid protein has been sequenced. The in vitro synthesized protein is taken up by intact chloroplasts, inserted into the thylakoid membrane and the transit peptide is cleaved off during this process. The mature protein is predicted to contain 69 amino acids, to form one membrane-spanning α-helix and to have its N-terminus at the stromal side of the thylakoid membrane. The protein showed similarity to the LHC, ELIP and PsbS proteins of higher plants, but more pronounced to the high-light-inducible proteins (HLIPs) of cyanobacteria and red algae, to which no homologue previously has been detected in higher plants. As for HLIP and ELIP, high light increases the mRNA levels of the corresponding gene. Sequence comparisons indicate that the protein may bind chlorophyll and form dimers in the thylakoid membrane. The level of expression of the protein seems to be far lower than that of normal PSI and PSII subunits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006365213954
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  • 20
    Electronic Resource
    Electronic Resource
    Retrotransposon Gypsy and genetic instability in Drosophila (review) (1991)
    Springer
    Genetica 85 (1991), S. 13-22 
    Details
    ISSN: 1573-6857
    Keywords: Drosophila melanogaster ; genetic instability ; gypsy ; mobile elements ; retrotransposons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The laboratory mutator strain (MS) has properties which can be characterized as genetic instability. It exhibits the high level of gypsy autonomous transposition in somatic and germ cells. This paper summarizes all the data concerning this system and gypsy itself that has been obtained in our works during the last years.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00056102
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