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  • American Geophysical Union  (91)
  • National Academy of Sciences  (47)
  • Molecular Diversity Preservation International  (36)
  • Frontiers Media  (32)
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  • 1
    Publication Date: 2017-04-04
    Description: Shallow slab devolatilization is not only witnessed through fluid expulsion at accretionary prisms, but is also evidenced by active serpentinite seamounts in the shallow fore-arc region of the Mariana convergent margin. Ocean Drilling Program (ODP) Leg 195 recovered serpentinized peridotites that present a unique opportunity to study the products of shallow level exchanges between the upper mantle and slab-derived fluids. Similar to samples recovered during ODP Leg 125, the protoliths of these fore-arc serpentinized peridotites are mantle harzburgites that have suffered large volume melt extraction (up to 25%) prior to interactions with fluids released from the downgoing Pacific Plate. Samples recovered from both ODP legs 125 and 195 show U-shaped rare earth element (REE) patterns and very low REE abundances (0.001–0.1 chondrites). Relative to global depleted mantle values these rocks typically have 1–2 orders of magnitude lower high field strength elements, REE, Th, and U contents. Interestingly, all fore-arc rocks thus far examined show extreme enrichments of fluid mobile elements (FME: B, As, Cs, Sb, Li). Because the elemental and B, Li, and Sr isotope systematics in these fore-arc serpentinites point to nonseawater-related processes, studies of elemental excesses and anomalous isotopic signatures allow assessment of how much of the subducted inventory is lost during the initial subduction process between 10 and 40 km. On the basis of similar but substantial enrichments of FME in the Mariana fore-arc samples recovered at ODP legs 125 and 195, we report large slab inventory depletions of B ( 75%), Cs ( 25%), As ( 15%), Li ( 15%), and Sb ( 8%); surprisingly low (generally less than 2%) depletions of Rb, Ba, Pb, U, Sr; and no depletions in REE and the high field strength elements (HFSE). Such slab-metasomatized mantle wedge materials may be dragged to depths of arc magma generation, as proposed by Tatsumi (1986) and Straub and Layne (2002) and thus represent an unexplored class of mantle material, different in its origins, physical properties and geochemical fingerprint from mantle rocks traditionally used in modeling a wide range of subduction zone processes.
    Description: Published
    Description: B09205
    Description: 2.3. TTC - Laboratori di chimica e fisica delle rocce
    Description: JCR Journal
    Description: reserved
    Keywords: Shallow slab fluid ; Mariana arc-basin ; 04. Solid Earth::04.04. Geology::04.04.05. Mineralogy and petrology ; 04. Solid Earth::04.04. Geology::04.04.07. Rock geochemistry
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 2
    Publication Date: 2017-04-04
    Description: Recent examinations of the chemical fluxes through convergent plate margins suggest the existence of significant mass imbalances for many key species: only 20–30% of the to-the-trench inventory of large-ion lithophile elements (LILE) can be accounted for by the magmatic outputs of volcanic arcs. Active serpentinite mud volcanism in the shallow forearc region of the Mariana convergent margin presents a unique opportunity to study a new outflux: the products of shallow-level exchanges between the upper mantle and slab-derived fluids. ODP Leg 125 recovered serpentinized harzburgites and dunites from three sites on the crests and flanks of the active Conical Seamount. These serpentinites have U-shaped rare earth element (REE) patterns, resembling those of boninites. U, Th, and the high field strength elements (HFSE) are highly depleted and vary in concentration by up to 2 orders of magnitude. The low U contents and positive Eu anomalies indicate that fluids from the subducting Pacific slab were probably reducing in nature. On the basis of substantial enrichments of fluid-mobile elements in serpentinized peridotites, we calculated very large slab inventory depletions of B (79%), Cs (32%), Li (18%), As (17%), and Sb (12%). Such highly enriched serpentinized peridotites dragged down to depths of arc magma generation may represent an unexplored reservoir that could help balance the input-output deficit of these elements as observed by Plank and Langmuir (1993, 1998) and others. Surprisingly, many species thought to be mobile in fluids, such as U, Ba, Rb, and to a lesser extent Sr and Pb, are not enriched in the rocks relative to the depleted mantle peridotites, and we estimate that only 1–2% of these elements leave the subducting slabs at depths of 10 to 40 km. Enrichments of these elements in volcanic front and behind-the-front arc lavas point to changes in slab fluid composition at greater depths.
    Description: Published
    Description: 1-24
    Description: partially_open
    Keywords: Serpentinite ; Ocean Drilling Program ; Forearc ; Mantle ; Marianas ; Subduction ; 04. Solid Earth::04.01. Earth Interior::04.01.02. Geological and geophysical evidences of deep processes ; 04. Solid Earth::04.04. Geology::04.04.05. Mineralogy and petrology ; 04. Solid Earth::04.04. Geology::04.04.07. Rock geochemistry ; 04. Solid Earth::04.07. Tectonophysics::04.07.06. Subduction related processes
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
    Format: 462 bytes
    Format: 2257702 bytes
    Format: text/html
    Format: application/pdf
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  • 3
    Publication Date: 2022-05-26
    Description: Author Posting. © American Geophysical Union, 2020. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 125(5), (2020): e2019JC015920, doi:10.1029/2019JC015920.
    Description: A major surface circulation feature of the Arctic Ocean is the Transpolar Drift (TPD), a current that transports river‐influenced shelf water from the Laptev and East Siberian Seas toward the center of the basin and Fram Strait. In 2015, the international GEOTRACES program included a high‐resolution pan‐Arctic survey of carbon, nutrients, and a suite of trace elements and isotopes (TEIs). The cruises bisected the TPD at two locations in the central basin, which were defined by maxima in meteoric water and dissolved organic carbon concentrations that spanned 600 km horizontally and ~25–50 m vertically. Dissolved TEIs such as Fe, Co, Ni, Cu, Hg, Nd, and Th, which are generally particle‐reactive but can be complexed by organic matter, were observed at concentrations much higher than expected for the open ocean setting. Other trace element concentrations such as Al, V, Ga, and Pb were lower than expected due to scavenging over the productive East Siberian and Laptev shelf seas. Using a combination of radionuclide tracers and ice drift modeling, the transport rate for the core of the TPD was estimated at 0.9 ± 0.4 Sv (106 m3 s−1). This rate was used to derive the mass flux for TEIs that were enriched in the TPD, revealing the importance of lateral transport in supplying materials beneath the ice to the central Arctic Ocean and potentially to the North Atlantic Ocean via Fram Strait. Continued intensification of the Arctic hydrologic cycle and permafrost degradation will likely lead to an increase in the flux of TEIs into the Arctic Ocean.
    Description: Funding for Arctic GEOTRACES was provided by the U.S. National Science Foundation, Swedish Research Council Formas, French Agence Nationale de la Recherche and LabexMER, Netherlands Organization for Scientific Research, and Independent Research Fund Denmark. Data from GEOTRACES cruises GN01 (HLY1502) and GN04 (PS94) have been archived at the Biological and Chemical Oceanography Data Management Office (Biological and Chemical Oceanography Data Management Office (BCO‐DMO); https://www.bco-dmo.org/deployment/638807) and PANGAEA (https://www.pangaea.de/?q=PS94&f.campaign%5B%5D=PS94) websites, respectively. The inorganic carbon data are available at the NOAA Ocean Carbon Data System (OCADS; doi:10.3334/CDIAC/OTG.CLIVAR_ARC01_33HQ20150809).
    Description: 2020-10-08
    Keywords: Arctic Ocean ; Transpolar Drift ; trace elements ; carbon ; nutrients ; GEOTRACES]
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 4
    Publication Date: 2015-02-23
    Description: Marine sponges are major habitat-forming organisms in coastal benthic communities and have an ancient origin in evolution history. Here, we report significant accumulation of polyphosphate (polyP) granules in three common sponge species of the Caribbean coral reef. The identity of the polyP granules was confirmed by energy-dispersive spectroscopy (EDS) and by the fluorescence properties of the granules. Microscopy images revealed that a large proportion of microbial cells associated with sponge hosts contained intracellular polyP granules. Cyanobacterial symbionts cultured from sponges were shown to accumulate polyP. We also amplified polyphosphate kinase (ppk) genes from sponge DNA and confirmed that the gene was expressed. Based on these findings, we propose here a potentially important phosphorus (P) sequestration pathway through symbiotic microorganisms of marine sponges. Considering the widespread sponge population and abundant microbial cells associated with them, this pathway is likely to have a significant impact on the P cycle in benthic ecosystems.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 5
    Publication Date: 2017-08-07
    Description: Extensive DNA sequence data have made it possible to reconstruct human evolutionary history in unprecedented detail. We introduce a method to study the past several hundred thousand years. Our results show that (i) the Neanderthal–Denisovan lineage declined to a small size just after separating from the modern lineage, (ii) Neanderthals and Denisovans separated soon thereafter, and (iii) the subsequent Neanderthal population was large and deeply subdivided. They also (iv) support previous estimates of gene flow from Neanderthals into modern Eurasians. These results suggest an archaic human diaspora early in the Middle Pleistocene.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 6
    Publication Date: 2015-04-13
    Description: The majority of bacteria in the natural environment live within the confines of a biofilm. The Gram-positive bacterium Bacillus subtilis forms biofilms that exhibit a characteristic wrinkled morphology and a highly hydrophobic surface. A critical component in generating these properties is the protein BslA, which forms a coat across the surface of the sessile community. We recently reported the structure of BslA, and noted the presence of a large surface-exposed hydrophobic patch. Such surface patches are also observed in the class of surface-active proteins known as hydrophobins, and are thought to mediate their interfacial activity. However, although functionally related to the hydrophobins, BslA shares no sequence nor structural similarity, and here we show that the mechanism of action is also distinct. Specifically, our results suggest that the amino acids making up the large, surface-exposed hydrophobic cap in the crystal structure are shielded in aqueous solution by adopting a random coil conformation, enabling the protein to be soluble and monomeric. At an interface, these cap residues refold, inserting the hydrophobic side chains into the air or oil phase and forming a three-stranded β-sheet. This form then self-assembles into a well-ordered 2D rectangular lattice that stabilizes the interface. By replacing a hydrophobic leucine in the center of the cap with a positively charged lysine, we changed the energetics of adsorption and disrupted the formation of the 2D lattice. This limited structural metamorphosis represents a previously unidentified environmentally responsive mechanism for interfacial stabilization by proteins.
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    Electronic ISSN: 1091-6490
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  • 7
    Publication Date: 2016-12-20
    Description: Leaf shape varies spectacularly among plants. Leaves are the primary source of photoassimilate in crop plants, and understanding the genetic basis of variation in leaf morphology is critical to improving agricultural productivity. Leaf shape played a unique role in cotton improvement, as breeders have selected for entire and lobed leaf morphs resulting from a single locus, okra (l-D1), which is responsible for the major leaf shapes in cotton. The l-D1 locus is not only of agricultural importance in cotton, but through pioneering chimeric and morphometric studies, it has contributed to fundamental knowledge about leaf development. Here we show that an HD-Zip transcription factor homologous to the LATE MERISTEM IDENTITY1 (LMI1) gene of Arabidopsis is the causal gene underlying the l-D1 locus. The classical okra leaf shape allele has a 133-bp tandem duplication in the promoter, correlated with elevated expression, whereas an 8-bp deletion in the third exon of the presumed wild-type normal allele causes a frame-shifted and truncated coding sequence. Our results indicate that subokra is the ancestral leaf shape of tetraploid cotton that gave rise to the okra allele and that normal is a derived mutant allele that came to predominate and define the leaf shape of cultivated cotton. Virus-induced gene silencing (VIGS) of the LMI1-like gene in an okra variety was sufficient to induce normal leaf formation. The developmental changes in leaves conferred by this gene are associated with a photosynthetic transcriptomic signature, substantiating its use by breeders to produce a superior cotton ideotype.
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  • 8
    Publication Date: 2020-02-03
    Description: Allostery can be manifested as a combination of repression and activation in multidomain proteins allowing for fine tuning of regulatory mechanisms. Here we have used single molecule fluorescence resonance energy transfer (smFRET) and molecular dynamics simulations to study the mechanism of allostery underlying negative cooperativity between the two agonists glutamate and glycine in the NMDA receptor. These data show that binding of one agonist leads to conformational flexibility and an increase in conformational spread at the second agonist site. Mutational and cross-linking studies show that the dimer–dimer interface at the agonist-binding domain mediates the allostery underlying the negative cooperativity. smFRET on the transmembrane segments shows that they are tightly coupled in the unliganded and single agonist-bound form and only upon binding both agonists the transmembrane domain explores looser packing which would facilitate activation.
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  • 9
    Publication Date: 2018-03-19
    Description: Here, we present a generalized method of guide RNA “tuning” that enables Cas9 to discriminate between two target sites that differ by a single-nucleotide polymorphism. We employ our methodology to generate an in vivo mutation prevention system in which Cas9 actively restricts the occurrence of undesired gain-of-function mutations within a population of engineered organisms. We further demonstrate that the system is scalable to a multitude of targets and that the general tuning and prevention concepts are portable across engineered Cas9 variants and Cas9 orthologs. Finally, we show that the mutation prevention system maintains robust activity even when placed within the complex environment of the mouse gastrointestinal tract.
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  • 10
    Publication Date: 2020-04-10
    Description: Heparin is the most widely prescribed biopharmaceutical in production globally. Its potent anticoagulant activity and safety makes it the drug of choice for preventing deep vein thrombosis and pulmonary embolism. In 2008, adulterated material was introduced into the heparin supply chain, resulting in several hundred deaths and demonstrating the need for alternate sources of heparin. Heparin is a fractionated form of heparan sulfate derived from animal sources, predominantly from connective tissue mast cells in pig mucosa. While the enzymes involved in heparin biosynthesis are identical to those for heparan sulfate, the factors regulating these enzymes are not understood. Examination of the promoter regions of all genes involved in heparin/heparan sulfate assembly uncovered a transcription factor-binding motif for ZNF263, a C2H2 zinc finger protein. CRISPR-mediated targeting and siRNA knockdown of ZNF263 in mammalian cell lines and human primary cells led to dramatically increased expression levels of HS3ST1, a key enzyme involved in imparting anticoagulant activity to heparin, and HS3ST3A1, another glucosaminyl 3-O-sulfotransferase expressed in cells. Enhanced 3-O-sulfation increased binding to antithrombin, which enhanced Factor Xa inhibition, and binding of neuropilin-1. Analysis of transcriptomics data showed distinctively low expression of ZNF263 in mast cells compared with other (non–heparin-producing) immune cells. These findings demonstrate a novel regulatory factor in heparan sulfate modification that could further advance the possibility of bioengineering anticoagulant heparin in cultured cells.
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    Topics: Biology , Medicine , Natural Sciences in General
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