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  • glycoprotein  (3)
  • Sediment transport  (2)
  • Springer  (3)
  • American Geophysical Union  (2)
  • Berlin: Deutsches Institut für Wirtschaftsforschung (DIW)
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Publisher
  • Springer  (3)
  • American Geophysical Union  (2)
  • Berlin: Deutsches Institut für Wirtschaftsforschung (DIW)
  • John Wiley & Sons  (2)
Years
  • 1
    ISSN: 1573-4986
    Keywords: Friend spleen focus-forming virus ; glycoprotein ; oligosaccharide processing ; SFFV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Normal rat kidney cells, non-productively infected with the anaemia-inducing variant of Friend spleen focus-forming virus (F-SFFVA), were metabolically labelled with [2-3H]mannose. The primary translation product of the viral envelope gene (env), representing a glycoprotein with an apparent molecularM r of 55 000 (gp55), was isolated from cell lysates by immunoaffinity chromatography and purified by preparative SDS/PAGE. Radiolabelled oligosaccharides, released from tryptic glycopeptides by treatment with endo-β-N-acetylglucosaminidase H, were characterized chromatographically, by enzymic digestion and by acetolysis. The results revealed that F-SFFVA gp55 obtained from this source carried predominantly oligomannose type sugar chains with five to nine mannoses. As a characteristic feature, glycans with seven to nine mannoses contained, in part, an additional glucose residue. Although the amount of glucosylated species found was higher in F-SFFVA gp55 (about 25% of total endo-H-sensitive oligosaccharides) than in gp55 of the corresponding polycythaemia-inducing variant (F-SFFVP, 16.3%), the overall glycosylation pattern of the F-SFFVA env product closely resembled that of F-SFFVP gp55 [Strubeet al. (1988)J Biol Chem 263:3762–71]. Hence, our results demonstrate that the different intracellular processing and transport of the primary F-SFFVA env product cannot be attributed to aberrant trimming of its oligomannose type glycans.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Glycoconjugate journal 14 (1997), S. 785-793 
    ISSN: 1573-4986
    Keywords: glycoprotein ; glycosylation ; gp120 ; HIV ; MALDI-TOF-MS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Neutral complex-type N-glycans of the envelope glycoprotein 120 of HIV-2, propagated in different host cells, display cell-type specific variations. In order to identify typical structural elements, glycans were analysed by gel filtration, by enzymic sequencing and, in part, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The characteristic substituents of di- tri- and tetraantennary carbohydrate units thus observed include N-acetyllactosamine repeats, bisecting N-acetylglucosamine and fucose linked to the chitobiose core as well as to N-acetyllactosamine antennae. Each glycoprotein preparation displayed a characteristic set of glycoforms. Abbreviations: endo H, endo-β-N-acetylglucosaminidase H; E-PHA, Phaseolus vulgaris agglutinin E4; GlcNAcOH, N-acetyl-glucosaminitol; gp120/HUT78(MOLT4/Mφ/PBL/U937), external envelope glycoprotein 120 of HIV-2, strain D194, propagated in HUT78 (MOLT4, Mφ, PBL, U937) cells; gu, glucose units; HPAEC, high-pH anion-exchange chromatography; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Mφ, human monocytes/macrophages; PBL, human peripheral blood lymphocytes; PNGase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase F
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  • 3
    ISSN: 1573-4986
    Keywords: glycoprotein ; glycopeptides ; N-linked oligosaccharides ; snake venom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In a previous study, we determined the structures of the glycans present in ancrod, a thrombin-like serine protease from the venom of the Malayan pit viperAgkistrodon rhodostoma (Pfeifferet al. (1992)Eur J Biochem 205:961–78). In order to allocate the various carbohydrate chains to distinctN-glycosylation sites of the molecule, we have now isolated individual glycopeptides. Peptide moieties were identified after deglycosylation with peptide-N 4-(N-acetyl-β-glucosaminyl)asparagine amidase F by amino acid analysis and sequencing. Liberated oligosaccharides were assigned to the previously deduced carbohydrate structures by high performance liquid chromatography. Although only quantitative differences were observed, the results indicate that each glycosylation site of ancrod carries its characteristic oligosaccharide pattern. Furthermore, all potential sites were shown to be substituted by carbohydrates.
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  • 4
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2005. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 110 (2005): C09025, doi:10.1029/2004JC002727.
    Description: A large flood of the Eel River, northern California, created a thick sediment deposit between water depths of 50 and 70 m in January 1997. The freshwater plume, however, confined sediment delivery to water depths shallower than 30 m. Mechanisms proposed to explain the apparent cross-shelf transport include dispersal by oceanographic currents, resuspension by energetic waves, and gravitationally forced transport of a thin layer of fluidized mud. Field observations indicate that these processes were all active but cannot determine their relative significance or whether these mechanisms alone explain the location, size, and timing of deposition. Approximately 30% of the sediment delivered by the Eel River is accounted for in the midshelf mud bed and inner shelf, but the fate of the remaining 70% is uncertain. A three-dimensional, hydrodynamic model was used to examine potential mechanisms of sediment transport on the Eel River shelf. The model includes suspended sediment transport and was modified to account for a thin, near-bed layer of fluidized mud. It was used to simulate flood dispersal on the Eel River shelf, to compare the relative importance of transport within the near-bed fluid mud layer to suspended sediment transport, and to evaluate sediment budgets for floods. Settling properties of fine-grained sediment, both within the flood plume and the fluid mud layer, critically impact depositional patterns. To a lesser degree, wind-driven ocean currents influence the volume of sediment that escapes the shelf, and wave magnitude affects the cross-shelf location of flood deposits. Though dilute suspension accounts for a large fraction of total flux, cross-shelf transport by gravitational forcing appears necessary to produce a midshelf mud deposit similar in volume, location, and timing to those seen offshore of the Eel River.
    Description: The Office of Naval Research’s Coastal Geoscience Program supported this through program N0014-01-1-008.
    Keywords: Flood sediment dispersal ; Northern California shelf ; Sediment transport
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 5
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2004. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 109 (2004): F02004, doi:10.1029/2003JF000096.
    Description: A 9 month time series of tripod-mounted optical and acoustic measurements of sediment concentration and bed elevation was used to examine depositional processes in relationship to hydrodynamic variables in the Hudson River estuary. A series of cores was also taken directly under and adjacent to the acoustic measurements to examine the relation between the depositional processes and the resulting fine-scale stratigraphy. The measurements reveal that deposition occurs as a result of sediment flux convergence behind a salinity front and that the accumulation rates are sufficient to deposit up to 25 cm of new high-porosity sediment in a single ebb-tidal phase. Subsequent dewatering and erosion reduces the thickness of the initial deposit to several centimeters. These depositional events were only observed on spring tides. Ten depositional events during two spring tidal cycles produced a seasonal deposit of 18 cm, consistent with estimates of seasonal deposition from cores. A proxy for near-bed suspended grain size variations was estimated from the combined acoustic and optical measurements, implying that the erosional processes resuspend only the finer-grained sediments, thus leaving behind silt and very fine grained sand beds. The thickness of the deposited homogenous clayey silt beds, and the vertical separation between beds interlaminated with silt and very fine sand, are roughly consistent with the acoustic measurements of changes in bed elevations during deposition and erosion. The variability in individual bed thickness is the result of variations of processes over an individual tidal cycle and is not a product of variations over the spring neap fortnightly timescale.
    Description: The authors would like to acknowledge the Hudson River Foundation, who provided funding for this work under grant 009/00A.
    Keywords: Sediment transport ; Estuarine processes ; Fluid mud
    Repository Name: Woods Hole Open Access Server
    Type: Article
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