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  • Chalcone synthase  (1)
  • fatty acids  (1)
  • Springer  (2)
  • American Chemical Society (ACS)
  • 1
    Electronic Resource
    Electronic Resource
    Properties of Luminescent Si Nanoparticles in Sol-Gel Matrices (1998)
    Springer
    Journal of sol gel science and technology 11 (1998), S. 267-272 
    Details
    ISSN: 1573-4846
    Keywords: silicon nanoparticles ; photoluminescence ; fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In this wor e preparation and properties of silica sol-gels incorporating luminescent Si nanocrystallites extracted from porous Si are described for the first time. These sol-gel/Si nanocrystallite composite materials are characterized by BET isotherm measurements, photoluminescence spectroscopy, and infrared spectroscopy. To stabilize the photoluminescence (PL) of Si crystallites within the silica matrix, a fatty acid (capric (C10), myristic (C14) or arachidic acid (C20)) is added as a passivation agent during the hydrolysis of tetraethoxysilane. The presence of the fatty acid is crucial to the long-term stability of the Si nanocrystallite luminescence, as the Si visible light emission remains essentially unchanged for more than a month when the fatty acid is present in the mixture but degrades quickly (within days) when absent. The thermal stability of the Si luminescence within the sol-gel is also reported. Fluorescence microscopy reveals that the light-emitting Si crystallites aggregate into micron-sized domains somewhat unevenly throughout the silica matrix. This distribution of Si crystallites can be improved by employing a surfactant, dioctyl sodium sulfosuccinate (DSS).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008606312748
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of the parsley chalcone-synthase promoter in response to different light qualities (1994)
    Springer
    Planta 193 (1994), S. 275-282 
    Details
    ISSN: 1432-2048
    Keywords: Chalcone synthase ; Footprinting in vivo ; Gene expression (transient) ; Light regulation (UV-B photoreceptor, blue-light photoreceptor) ; Petroselinum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the chalcone synthase (chs) promoter from parsley [Petroselinum crispum Miller (A.W. Hill)] for the existence of separate promoter elements responsible for transcriptional activation of the chs gene by UV-B and by blue light. A combination of in-vivo footprinting in parsley cells and light-induced transient expression assays with different chs promoter constructs in parsley protoplasts was used. Dark controls and bluelight-irradiated cells gave identical in-vivo footprints on the chs promoter. Pre-irradiation with blue light prior to a UV-B-light pulse is known to cause a shift in the timing of UV-B-light-induced increase in chs transcription rates. This shift was also manifested on the DNA template, since UV-B-light-induced in-vivo footprints in cells pretreated with blue light were detected earlier than in cells which had been irradiated with a UV-B-light pulse only. Although there was a clear shift in the timing of footprint appearance, the patterns of footprinting did not change. Light-induced transient-expression assays revealed that the shortest tested chs promoter which retained any light responsiveness, was sufficient for mediating both induction by UV light and the blue-light-mediated kinetic shift. These findings argue against a spatial separation of UV-B- and blue-light-responsive elements on the chs promoter. We interpret these data by postulating that the signal transduction pathways originating from the excitation of UV-B- and blue-light receptors merge at the chs promoter, or somewhere between light perception and protein-DNA interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00192541
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