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  • Chalcone synthase  (1)
  • cadmium sulfide  (1)
  • Springer  (2)
  • American Chemical Society (ACS)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of cluster science 11 (2000), S. 359-372 
    ISSN: 1572-8862
    Keywords: cadmium sulfide ; quantum dots ; DNA stabilized
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract In this work, the conditions required to transform quantum-confined cadmium sulfide (Q-CdS) nanoparticles stabilized by calf thymus deoxyribonucleic acid from deep trap photoluminescent states to “band edge”-type luminescence are probed. The presence of fivefold excess sulfide relative to cadmium concentration during cluster synthesis, followed by mild heating at 80°C, results in the desired transformation of the Q-CdS emission spectrum. We also indirectly analyze the accompanying structural changes in the polymeric stabilizer, accomplished in this case by use of well-known spectrofluorometric methods with the dyes ethidium bromide and trisphenanthroline ruthenium(II).
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Chalcone synthase ; Footprinting in vivo ; Gene expression (transient) ; Light regulation (UV-B photoreceptor, blue-light photoreceptor) ; Petroselinum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the chalcone synthase (chs) promoter from parsley [Petroselinum crispum Miller (A.W. Hill)] for the existence of separate promoter elements responsible for transcriptional activation of the chs gene by UV-B and by blue light. A combination of in-vivo footprinting in parsley cells and light-induced transient expression assays with different chs promoter constructs in parsley protoplasts was used. Dark controls and bluelight-irradiated cells gave identical in-vivo footprints on the chs promoter. Pre-irradiation with blue light prior to a UV-B-light pulse is known to cause a shift in the timing of UV-B-light-induced increase in chs transcription rates. This shift was also manifested on the DNA template, since UV-B-light-induced in-vivo footprints in cells pretreated with blue light were detected earlier than in cells which had been irradiated with a UV-B-light pulse only. Although there was a clear shift in the timing of footprint appearance, the patterns of footprinting did not change. Light-induced transient-expression assays revealed that the shortest tested chs promoter which retained any light responsiveness, was sufficient for mediating both induction by UV light and the blue-light-mediated kinetic shift. These findings argue against a spatial separation of UV-B- and blue-light-responsive elements on the chs promoter. We interpret these data by postulating that the signal transduction pathways originating from the excitation of UV-B- and blue-light receptors merge at the chs promoter, or somewhere between light perception and protein-DNA interaction.
    Type of Medium: Electronic Resource
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