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  • Column-switching  (1)
  • Copper  (1)
  • Springer  (2)
  • American Chemical Society (ACS)
  • MDPI Publishing
  • Mineralogical Society of Great Britain and Ireland
Collection
Publisher
  • Springer  (2)
  • American Chemical Society (ACS)
  • MDPI Publishing
  • Mineralogical Society of Great Britain and Ireland
Years
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 51 (1979), S. 19-26 
    ISSN: 1573-5036
    Keywords: Bacteria ; Cofactors ; Copper ; Ethylene ; FMN ; Light ; Methionine ; Plant pathogens ; Pseudomonas ; Substrate ; Temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Methionine, up to 10−3 M, added to a basal medium enhanced bacterial ethylene production in 14 of the 20 bacteria tested. The effects of substrate, cofactors, light, and temperature on ethylene production byPseudomonas solanacearum #25 revealed that the greatest effect occurred when 10−5 M methionine and 10−4 M FMN were combined, from which 4.10μl/l of ethylene were produced. Higher levels of methionine resulted in production of high levels of non-enzymically produced ethylene and death of the bacteria. This non-enzymic production of ethylene was eliminated in the dark. Copper had no effect upon ethylene production. Twenty-nine and 35°C were inhibitory, whereas 19°C appeared to be near optimum for ethylene production.Pseudomonas solanacaerum #25 and some other bacteria are capable of ethylene production and methionine and FMN enhance this production.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1612-1112
    Keywords: Terbutaline ; Plasma ; Automated HPLC ; Column-switching ; Electrochemical detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An automated method based on liquid chromatography has been developed for the determination of terbutaline in human plasma in the range of 5–50 pmole·ml−1. The necessary sensitivity and selectivity was obtained by using electrochemical detection and a microprocessor-controlled column switching system. A combination of three columns was used: a C8 type for pre-separation, a C18 type for trapping and, for final separation, a strongly acidic ion exchanger. The accuracy of the method was examined by comparison with a method based on gas chromatography — mass spectrometry. The overall precision was ±3.5% and ±2.2% respectively at 5 and 50 pmole·ml−1. The total absolute recovery for terbutaline and internal standard at the above concentration levels were in the range 85–106%.
    Type of Medium: Electronic Resource
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