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  • Immunocytochemistry  (2)
  • Key words RFLPs  (2)
  • Nudibranchia  (2)
  • Springer  (6)
  • American Chemical Society (ACS)
  • American Institute of Physics (AIP)
  • International Union of Crystallography (IUCr)
  • Oxford University Press
Collection
Keywords
Publisher
  • Springer  (6)
  • American Chemical Society (ACS)
  • American Institute of Physics (AIP)
  • International Union of Crystallography (IUCr)
  • Oxford University Press
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Years
  • 1
    Electronic Resource
    Electronic Resource
    A comparative map of wild rice (Zizania palustris L. 2n=2x=30) (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 793-799 
    Details
    ISSN: 1432-2242
    Keywords: Key words RFLPs ; Synteny ; Oryza ; Oat ; Map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We present the first genetic map of wild rice (Zizania palustris L., 2n=2x=30), a native aquatic grain of northern North America. This map is composed principally of previously mapped RFLP (restriction fragment length polymorphism) genetic markers from rice (Oryza sativa 2n=2x=24). The map is important as a foundation for genetic and crop improvement studies as well as a reference for genome organization comparisons among species of Gramineae. A comparative mapping approach with rice is especially useful because wild rice is grouped in the same subfamily, Oryzoideae, and no other mapping comparison has yet been made within the subfamily. As rice is the reference point for mapping and gene cloning in cereals, establishing a consensus map within the subfamily identifies conserved and unique regions. The genomes of wild rice and rice differ in total DNA content (wild rice has twice that of rice) and the number of chromosome pairs (wild rice=15 versus rice=12). The wild rice linkage map reported herein consists of 121 RFLP markers on 16 linkage groups spanning 1805 cM. Two linkage groups consist of only two markers. Colinear markers were found representing all rice linkage groups except #12. The majority of rice loci mapped to colinearly arranged arrays in wild rice (92 of 118). Features of the map include duplication of portions of three rice linkage groups and three possible translocations. The map gives basic information on the composition of the wild rice genome and provides tools to assist in the domestication of this important food source.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051298
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  • 2
    Electronic Resource
    Electronic Resource
    A comparative map of wild rice (Zizania palustris L. 2n=2x=30) (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 677-684 
    Details
    ISSN: 1432-2242
    Keywords: Key words RFLPs ; Synteny ; Oryza ; Oat ; Map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We present the first genetic map of wild rice (Zizania palustris L., 2n=2x=30), a native aquatic grain of northern North America. The map is composed principally of previously mapped RFLP (restriction fragment length polymorphism) genetic markers from rice (Oryza sativa 2n=2x=24). The map is important as a foundation for genetic and crop improvement studies, as well as a reference for genome organization comparisons among Gramineae species. A comparative mapping approach with rice is especially useful because wild rice is grouped in the same subfamily, Oryzoideae, and no other mapping comparison has yet been made within the subfamily. As rice is the reference point for mapping and gene cloning in cereals, establishing a consensus map within the subfamily identifies conserved and unique regions. The genomes of wild rice and rice differ in total DNA content (wild rice has twice that of rice) and chromosome pairs (wild rice=15 versus rice=12). The wild rice linkage map reported herein consists of 121 RFLP markers on 16 linkage groups spanning 1805 cM. Two linkage groups consist of only two markers. Colinear markers were found representing all rice linkage groups except #12. The majority of rice loci mapped to colinearly arranged arrays in wild rice (92 of 118). Features of the map include duplication of portions of three rice linkage groups and three possible translocations. The map gives basic information on the composition of the wild rice genome and provides tools to assist in the domestication of this important food source.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051530
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  • 3
    Electronic Resource
    Electronic Resource
    The Golgi apparatus in epidermal mucoid and ellipsoid-vacuolate cells ofAeolidia papillosa andCoryphella rufibranchialis (Nudibranchia) (1980)
    Springer
    Protoplasma 102 (1980), S. 217-233 
    Details
    ISSN: 1615-6102
    Keywords: Epidermis ; Golgi apparatus ; Nudibranchia ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The epidermal cell layer of the apical end of the ceras was investigated in two species of aeolid nudibranchs. Based on cellular inclusions, mostly two cell types were found: mucoid and ellipsoid-vacuolate cells. Mucoid cells ofCoryphella rufibranchialis have large heterogeneous and fibrillar secretory granules whereas inAeolidia papillosa, the granules are homogeneous, but vary in electron density from one cell to another. Ellipsoid-vacuolate cells contained large quantities of small vacuoles with an included ellipsoidal structure. Both species contained very numerous ellipsoid-vacuolate cells. Secretory granules and ellipsoid-vacuoles appear to arise from the Golgi apparatus and these contents stain with PAS, suggesting a polysaccharide composition. Mucoid cells contained both secretory granules and ellipsoid-vacuoles which may arise from the same Golgi apparatus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279589
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  • 4
    Electronic Resource
    Electronic Resource
    Light and electron microscopic cytochemistry of the epidermal mucoid cells ofAeolidia papillosa andCoryphella rufibranchialis (Mollusca, Nudibranchia) (1983)
    Springer
    Protoplasma 114 (1983), S. 14-22 
    Details
    ISSN: 1615-6102
    Keywords: Cytochemistry ; Epidermis ; Golgi apparatus ; Mucoid cells ; Nudibranchia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The epidermal mucoid cells in the cerata ofAeolidia papillosa andCoryphella rufibranchialis were cytochemically tested to determine the composition of their secretory products. The PAS, alcian blue, PA-TSC-SP and high iron diamine stains were used to determine the presence of acidic, sulphated, and periodate-reactive groups on the mucopolysaccharides. The secretory granules in mucoid cells ofA. papillosa consisted of periodate-reactive mucopolysaccharides with acid groups other than sulphates. Each granule also contained fibrils which were not oxidized by periodic acid. The mucoid secretory granules inC. rufibranchialis contained weakly acidic sulphated mucopolysaccharides, but no periodate-reactive polysaccharide groups. In both aeolid nudibranchs the Golgi apparatus at the base of the mucoid cells contained products which stained the same as those in the secretory granules, confirming that the Golgi apparatus is involved in the synthesis of mucoid secretory products. In addition the mucoid cell Golgi complex ofC. rufibranchialis also gave rise to ellipsoid vacuoles which contained sulphated mucopolysaccharides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279864
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  • 5
    Electronic Resource
    Electronic Resource
    Stereological study of gonadotropes in the frog, Rana pipiens, after GnRH stimulation in vitro (1990)
    Springer
    Cell & tissue research 262 (1990), S. 171-176 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Gonadotropes ; Morphometry ; Stereology ; Rana pipiens (Anura)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Previous physiological results have indicated the existence of two releasable pools of gonadotropins in amphibian pituitaries: an acute releasable pool that appears independent of protein synthesis, and a storage pool involved in chronic release that depends on protein synthesis. To elucidate the ultrastructural localization of these pools and the morphological changes induced in gonadotrope cells after treatment with gonadotropin-releasing hormone, we carried out a morphometric study of immuno-identified gonadotrope cells using an in vitro superfusion system. Treatment with gonadotropin-releasing hormone induced a degranulation of small (110–255 nm) and medium (236–360 nm) secretory granules as well as hypertrophy of the endoplasmic reticulum and Golgi complex. Simultaneous incubation with gonadotropin-releasing hormone and cycloheximide inhibited the release of secretory granules although the endoplasmic reticulum and Golgi complex were hypertrophied. These morphological results strongly suggest: (1) that gonadotropin-releasing hormone induces degranulation and hypertrophy of the biosynthetic machinery in gonadotrope cells; and (2) that the activation of the endoplasmic reticulum and Golgi complex by stimulation with gonadotropin-releasing hormone is independent of protein synthesis, while the release of secretory granules is protein synthesis-dependent. In addition, the second or “storage” pool of gonadotropin is associated mainly with the small and medium secretory granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327759
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  • 6
    Electronic Resource
    Electronic Resource
    Immunocytochemical and ultrastructural study of the frog (Rana pipiens) pars distalis with special reference to folliculo-stellate cell function during in vitro superfusion (1989)
    Springer
    Cell & tissue research 256 (1989), S. 623-630 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Prolactin cells ; Gonadotropic cells ; ACTH cells ; Folliculo-stellate cells ; Rana pipiens (Anura)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The colloidal gold immunocytochemical technique was used to determine the ultrastructural features of the glandular cells in the pituitaries of male frogs, Rana pipiens, both in vivo and after superfusion in vitro. Specific reactions to antisera against bullfrog gonadotropins, human prolactin, and synthetic 1–39 corticotropin allowed identification of the 3 corresponding types of glandular cells. No immunoreaction was obtained with antisera against human or ovine-growth hormone, human β-thyrotropin hormone, and bovine S-100 protein. General morphological features of these immunocytochemically identified glandular cells were similar to those of equivalent cells previously described in other amphibian species. Non-glandular folliculo-stellate cells were distinctive. In freshly removed pituitaries, these folliculo-stellate cells contained lysosome-like structures, but did not show phagocytic vacuoles in the cytoplasm; they contained many mitochondria, and the Golgi complex and endoplasmic reticulum were relatively undeveloped. After 4 or 18 h of superfusion, some immunoreactive gonadotropic, prolactin, and corticotropic cells showed degeneration and destruction. In the same gland, folliculo-stellate cells retained a viable appearance, but showed phagocytic vacuoles containing secretory granule-like structures which were immunoreactive to gonadotropic, prolactin, and corticotropic antibodies. Some folliculo-stellate cells showed phagocytic vacuoles containing complete glandular cells. These results suggest that superfusion causes a destruction of some of the glandular cells, and that folliculo-stellate cells act as phagocytes when cellular debris or moribund cells are present in the intercellular space in the pituitary parenchyma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225612
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