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  • RFLP  (49)
  • Triticum aestivum  (31)
  • Springer  (76)
  • American Chemical Society
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  • Springer  (76)
  • American Chemical Society
  • Elsevier  (8)
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  • 1
    Electronic Resource
    Electronic Resource
    Yield response of spring wheat cultivars (Triticum aestivum and T. turgidum) to inoculation with Azospirillum brasilense under field conditions (1987)
    Springer
    Biology and fertility of soils 4 (1987), S. 27-35 
    Details
    ISSN: 1432-0789
    Keywords: Triticum aestivum ; T. turgidum ; Nitrogen fixation ; Field inoculation ; Acetylene reduction assay (ARA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eight commercial Israeli spring wheat cultivars (six Triticum aestivum and two T. turgidum) grown with 40 and 120 kg N/ha were tested for responses to inoculation with Azospirillum brasilense. At the low level of N fertilization (40 kg/ha), five cultivars showed significant increases in plant dry weight measured at the milky ripe stage; however, by maturation only the cultivar “Miriam” showed a significant increase in grain yield. Two cultivars, which had shown a positive inoculation effect at the earlier stages, had a significant decrease in grain yield. No significant effect of inoculation was found at the high N level. To confirm those results, four wheat (T. aestivum) cultivars were tested separately over 4 years in 4 different locations under varying N levels. Only Miriam showed a consistently positive effect of Azospirillum inoculation on grain yield. Inoculation increased the number of roots per plant on Miriam compared with uninoculated plants. This effect was found at all N levels. Nutrient (N, P and K) accumulation and number of fertile tillers per unit area were also enhanced by Azospirillum, but these parameters were greatly affected by the level of applied N. It is suggested that the positive response of the spring wheat cultivar “Miriam” to Azospirillum inoculation is due to its capacity to escape water stresses at the end of the growth season.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280347
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  • 2
    Electronic Resource
    Electronic Resource
    Sequence-tagged-sites (STSs) of cDNA clones in Cryptomeria japonica and their evaluation as molecular markers in conifers (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 764-772 
    Details
    ISSN: 1432-2242
    Keywords: Key words Linkage map ; RFLP ; STS ; Conifers ; Cryptomeria japonica ; cDNA ; Polymorphism ; Phylogeny ; Homology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have generated 66 sequence-tagged-site (STS) markers from cDNA clones of Cryptomeria japonica, and 60% of them have already been mapped into C. japonica linkage groups. All of the STS markers showed a single fragment following polymerase chain reaction (PCR) amplification. We investigated by polymorphism of these STS markers in a mapped F2 population and 15 plus trees by means of a restriction endonuclease analysis. Polymorphism levels were 10.6% and 22.7% in the F2 population and the 15 plus trees, respectively. PCR amplification levels of the 66 STS markers in 14 conifer species varied depending on their genetic relationship with C. japonica. Taxodium, which is closely related to C. japonica, had the most amplifications (31.82%), followed by Sequoiadendron giganteum, which is of the same family. The average proportion of PCR amplifications in each family gradually declined in the following order: from Taxodiaceae to Cuppresaceae, Sciadopityaceae, Pinaceae, and Taxaceae. These results are in general agreement with a molecular phylogenetic relationship based on chloroplast DNA. The 66 STS markers will be useful as on anchor point for genome mapping and population genetics, and some of them will also be useful when studying other conifers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050476
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  • 3
    Electronic Resource
    Electronic Resource
    The semidwarf gene, sd-1, of rice (Oryza sativa L.). II. Molecular mapping and marker-assisted selection (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 54-59 
    Details
    ISSN: 1432-2242
    Keywords: Rice ; Semidwarf gene (sd-1) ; RFLP ; Molecular marker ; Marker-assisted selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To establish the location of the semidwarf gene, sd-1, the anthocyanin activator (A), purple node (Pn), purple auricle (Pau), and the isozyme locus, EstI-2, in relation to DNA markers on the molecular linkage map of rice, 20 RFLP markers, previously mapped to the central region of chromosome 1 (McCouch et al. 1988), were mapped onto an F2 population derived from the cross Taichung 65 (A,Pn,Pau)/Taichung 65 (sd-1). sd-1 and EstI-2 were determined to be linked most tightly to RFLP markers RG 109 and RG 220, which cosegregated with each other. The distance between these RFLP markers and sd-1 was estimated to be 0.8 cM, based on an observed recombination value of 0.8%. The order of genes and markers in this region of chromosome 1 was determined to be sd-1 — (EstI-2 — RG220 — RG109) — RG381 — A — Pn — Pau. To test the efficacy of selection for sd-1 based on these linked markers, 50-day-old F2 seedlings derived from another cross, Milyang 23/Gihobyeo, were analyzed for marker genotype. At this age, the semidwarf character could not be clearly detected based on phenotype. In addition, plant height was normally distributed in this population, making it difficult to unambiguously identify plants carrying sd-1. Thirteen seedlings homozygous for the sd-1-associated allele at EstI-2, RG220 and RG109, and 13 seedlings homozygous for the Sd-1-associated allele at all three marker loci were selected for further genetic analysis. At 20 days after heading, the culm lengths of these 26 plants were measured and the expected phenotype was confirmed in every case. These 26 plants were then selfed for four generations and F6 lines were again evaluated to determine whether any recombination among the three molecular markers, or between these markers and the sd-1 gene, could be detected. No recombinants were identified, confirming the tight linkage of these loci and the usefulness of genotypic selection for this recessive semidwarf character prior to the time when it can be evaluated based on phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226982
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  • 4
    Electronic Resource
    Electronic Resource
    A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 835-840 
    Details
    ISSN: 1432-2242
    Keywords: RFLP ; RAPD ; Linkage map ; Sugi (Cryptomeria japonica) ; Three-generation pedigree
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A linkage map for sugi was constructed on the basis of restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and isozyme loci using a three-generation pedigree prepared for genetic analysis of heartwood color. A total of 128 RFLP (123 cDNA and 5 genomic probes), 33 RAPD, 2 isozyme, and 1 morphological (dwarf) loci segregated in 73 progeny. Of the 164 segregating loci, 145 loci were distributed in 20 linkage groups. Of these loci, 91 with confirmed map positions were assigned to 13 linkage groups, covering a total of 887.3 cM. A clustering of markers with distorted segregation was observed in 6 linkage groups. In the four clusters, distortions with a reduction in the number of homozygotes from one parent only were found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222019
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  • 5
    Electronic Resource
    Electronic Resource
    Development of diagnostic PCR markers closely linked to the tomato powdery mildew resistance gene Ol-1 on chromosome 6 of tomato (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 918-924 
    Details
    ISSN: 1432-2242
    Keywords: Keywords Resistance ; Tomato powdery mildew ; Tomato ; Mapping ; Oidium lycopersicum ; RFLP ; Sequence characterised amplified region (SCAR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Lycopersicon hirsutum G1.1560 is a wild accession of tomato that shows resistance to Oidium lycopersicum, a frequently occurring tomato powdery mildew. This resistance is largely controlled by an incompletely dominant gene Ol-1 near the Aps-1 locus in the vicinity of the resistance genes Mi and Cf-2/Cf-5. Using a new F2 population (n=150) segregating for resistance, we mapped the Ol-1 gene more accurately to a location between the RFLP markers TG153 and TG164. Furthermore, in saturating the Ol-1 region with more molecular markers using bulked segregant analysis, we were able to identify five RAPDs associated with the resistance. These RAPDs were then sequenced and converted into SCAR markers: SCAB01 and SCAF10 were L. hirsutum-specific; SCAE16, SCAG11 and SCAK16 were L. esculentum-specific. By linkage analysis a dense integrated map comprising RFLP and SCAR markers near Ol-1 was obtained. This will facilitate a map-based cloning approach for Ol-1 and marker-assisted selection for powdery mildew resistance in tomato breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051562
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  • 6
    Electronic Resource
    Electronic Resource
    Lycopersicon esculentum lines containing small overlapping introgressions from L. pennellii (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 1027-1034 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon esculentum ; L. pennellii ; RFLP ; Introgression lines ; Breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The objective of this project was to introgress small overlapping chromosome segments which cover the genome of L. pennellii into Lycopersicon esculentum lines. The interspecific hybrid was backcrossed to L. esculentum, and a map of 981 cM, based on 146 molecular markers covering the entire genome, was produced. A similar backcross 1 population was selfed for six generations, under strong selection for cultivated tomato phenotypes, to produce 120 introgression lines. The introgression lines were assayed for the above-mentioned molecular markers, and 21 lines covering 936 cM of L. pennellii, with an average introgression of 86 cM, were selected to provide a resource for the mapping of new DNA clones. The rest of the lines have shorter introgressions consisting of specific regions with an average size of 38 cM. The proportion of the L. pennellii genome in the introgression lines was lower than expected (252 cM) because of strong selection against the wild-parent phenotype. The mean introgression rate for ends of linkage groups in the 120 lines was 3 times higher than for other regions of the genome. The introgression lines can assist in RFLP-based gene cloning by allowing the rapid selection of DNA markers that map to specific chromosome segments. The introgression lines also provide a base population for the mapping and breeding for quantitative traits such as salt and drought tolerance that characterize the wild species L. pennellii.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232968
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  • 7
    Electronic Resource
    Electronic Resource
    Sequence-tagged sites (STSs) as standard landmarkers in the rice genome (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 728-734 
    Details
    ISSN: 1432-2242
    Keywords: STS ; RFLP ; Rice ; Genetic map ; Coding region
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Generating sequence-tagged sites (STSs) is a prerequisite to convert a genetic map to a physical map. With the help of sequence information from these STSs one can also isolate specific genes. For these purposes, we have designed PCR primer sets, of 20 bases each, by reference to sequences of restriction fragment length polymorphism (RFLP) landmarkers consisting of rice genomic clones. These markers were evenly distributed over the 12 chromosomes and were shown to be single copy by Southern-blot analysis. With improved PCR protocols, 63 standard STS landmarkers in the rice genome were generated. Similarity searches of all partial sequences of RFLP landmarkers by the FASTA algorithm showed that 2 of the 63 RFLP landmarkers, G357 and G385, contained part of the ORFs of aspartate aminotransferase and protein kinase, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223712
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular changes in protoplast-derived rice plants (1990)
    Springer
    Molecular genetics and genomics 223 (1990), S. 324-328 
    Details
    ISSN: 1617-4623
    Keywords: Rice ; Protoplast ; RFLP ; Methylation ; Polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To determine whether regeneration of rice plants from protoplast culture induces DNA polymorphisms, progeny plants from direct regenerants of such cultures were examined for restriction fragment length polymorphisms (RFLP analysis). Significantly increased levels of DNA polymorphism were found compared with those in non-tissue culture control plants. Analysis with gene sequences representative of different functional domains, revealed that such polymorphisms are apparently widespread and not associated with any particular region. Analysis by comparative digestion with both methylation-sensitive and insensitive restriction enzymes revealed that methylation changes cannot be regarded as a major factor in the induction of these DNA polymorphisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265070
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  • 9
    Electronic Resource
    Electronic Resource
    Analysis of Chinese Spring regenerants obtained from short- and long-term wheat somatic embryogenesis (1995)
    Springer
    Euphytica 82 (1995), S. 263-268 
    Details
    ISSN: 1573-5060
    Keywords: somaclonal variation ; somatic embryogenesis ; tissue culture ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Somatic embryogenesis was initiated from ‘immature embryos’ on Murashige-Skoog (MS) medium plus 2 mg.l-1 2,4-dichlorophenoxyacetic acid, 2% sucrose and 0.6% agarose. Somatic embryos were isolated and regenerated into whole green plants on MS medium devoid of 2,4-D. These regenerants were previously demonstrated to differ in their mitochondrial DNA organization. In order to estimate their characteristics three progenies of short-term culture regenerants and three progenies of long-term culture regenerants were analyzed and compared to the parental line. These somaclones obtained from the wheat variety Chinese Spring were evaluated for variation of 13 agronomic and morphological quantitative characters in comparison to the parental line. Significant variation was observed for plant height, spike length, main tiller diameter, between the somaclones regenerated from long-term culture and their parent. Differences were observed to increase with the duration of culture, leading to a significant modification of the structure of the plants. Several changes occurred during the somatic tissue cultures, but to a lesser extent than has previously been described in the literature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029569
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  • 10
    Electronic Resource
    Electronic Resource
    Gram-negative bacterial flora on the root surface of wheat (Triticum aestivum) grown under different soil conditions (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 273-281 
    Details
    ISSN: 1432-0789
    Keywords: Wheat ; Triticum aestivum ; Rhizosphere ; Soil microflora ; Gram-negative bacteria ; API 20 NE ; Flavobacterium spp ; Cytophaga
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We identified 161 Gram-negative bacterial strains isolated from the root surface of wheat grown under different soil conditions. The strains were divided into seven groups based on major morphological and physiological properties. Taxonomic allocation of the groups was verified by guanine+cytosine contents of DNA. Except for one group, which may be assumed to include bacteria belonging to the genera Flavobacterium and Cytophaga, the various groups were taxonomically united. The distribution of the groups changed with soil improvement. Pseudomonads predominated in unimproved soil, but Flavobacterium and Cytophaga spp. were predominant in the most improved soil. As all the strains were non-fermentative by Hugh and Leifson's test, API 20NE identification was applied. However, many strains were misidentified by this system, especially in the Flavobacterium and Cytophaga spp. group. For ecological studies, the strains were classified to species level by the API 20 NE system and by the results of a combination of guanine+cytosine (mol%) and isoprenoid quinone data. The pattern of distribution of the bacteria on the root surface of wheat varied at species level within one genus depending on soil conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335955
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