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  • Beta vulgaris  (13)
  • Ultrastructure  (7)
  • Springer  (20)
  • American Chemical Society
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  • Springer  (20)
  • American Chemical Society
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  • 1
    Electronic Resource
    Electronic Resource
    Investigations on an interspecific hybrid involving three species of the genus Beta, with special reference to isozyme polymorphism (1986)
    Springer
    Theoretical and applied genetics 73 (1986), S. 261-266 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Sugar beet ; Beta lomatogona ; Beta procumbens ; Interspecific hybrid ; Isozyme polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A tetraploid (2n=36) interspecific hybrid was obtained involving three species belonging to three different sections of Beta. The hybrid was highly sterile and did not show apomixis. At meiosis, up to nine bivalents were observed, most probably resulting from autosyndesis of the chromosomes of Beta lomatogona. For nine isozyme systems, individual enzyme expression was investigated in the parental species and in the hybrids. No silencing of genes or genomes was observed. In the case of some polymeric enzymes interspecific heteropolymers could be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289283
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular characterization of mitochondrial DNA of different subtypes of male-sterile cytoplasms of the sugar beet Beta vulgaris L. (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 11-16 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Cytoplasmic male sterility ; mtDNA ; Restriction fragment patterns ; Filter hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) DNA from eight cytoplasmic male-sterile (cms) lines of sugar beet from different breeding stations was investigated by restriction fragment analysis and Southern hybridization. All cms lines showed similar but not identical restriction and hybridization signal patterns, readily distinguishable from those of fertile (N) cytoplasm. Digestion of the mtDNA with BamHI, EcoRI, SalI, and XhoI revealed distinct differences between the sterile lines, and six subtypes of the S cytoplasm could be distinguished. Differences between the sterile lines were confirmed by hybridization with a $$\overline{\overline {COX}} II$$ gene probe revealing minor, line-specific hybridization signals. The data presented provide evidence for the existence of considerable variation within the only commercially used source of cms in the sugar beet, the Owen's type of cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231271
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  • 3
    Electronic Resource
    Electronic Resource
    Monosomic additions in beet (Beta vulgaris) carrying extra chromosomes of B. procumbens (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 656-664 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Beta procumbens ; Alien monosomic additions ; Plant development in vivo ; Development in vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Alien monosomic additions in beet (Beta vulgaris), each carrying one of the nine chromosomes of B. procumbens, were grown in vivo and in vitro to study the effect of the alien chromosomes on plant development. All additional chromosomes caused a reduction of the growth rate in vivo, which, in one case was so strong that some of the plants died as seedlings. In general, the morphological plant characteristics were not very useful to distinguish the addition types; this could have been the results of the wide variation in the recipient parent. However, some developmental characteristics proved to be highly chromosome-specific; for plants in vivo this was annuality, in combination with early or late flowering. If grown in vitro, chromosome specificity was observed for growth type (rosette or elongated stem), occurrence and rate of vitrification, occurrence and morphology of wound callus, formation of additional meristems on the midribs of leaves, formation of roots and a specific reaction to benzylaminopurine (BAP) the medium. Two chromosome types of B. procumbens caused resistance to the beet cyst nematode.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303509
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  • 4
    Electronic Resource
    Electronic Resource
    Inheritance of resistance to beet necrotic yellow vein virus in Beta vulgaris conferred by a second gene for resistance (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 740-746 
    Details
    ISSN: 1432-2242
    Keywords: Key words Beet necrotic yellow vein virus ; Beta vulgaris ; Inheritance ; Resistance genes ; Rhizomania ; STS markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Rhizomania is a serious disease of sugar beet, caused by beet necrotic yellow vein virus (BNYVV). The disease can only be controlled by the use of resistant cultivars. The accession Holly contains a single dominant gene for resistance, called Rz. The identification of a locus for resistance that differs from Rz would provide possibilities to produce cultivars with multiple resistance to BNYVV. Inheritance of resistance to BNYVV was studied by screening progenies of crosses between resistant plants of the accessions Beta vulgaris subsp. maritima WB42 and B. vulgaris subsp. vulgaris Holly-1–4 or R104. Observed and expected segregation ratios were compared to elucidate whether the resistance genes in the three accessions are alleles or situated on different loci. STS markers, linked to the genes for resistance, were used to study the segregation in more detail. The results demonstrated that the genes for resistance to BNYVV inHolly-1-4 and WB42 are closely linked. The gene for resistance in R104 is at the same locus as in Holly-1-4, and also closely linked to the gene in WB42. As the Holly resistance gene has been named Rz, the name Rz2 is proposed to refer to the resistance gene in WB42. Consequently, the gene Rz should be referred to as Rz1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051292
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  • 5
    Electronic Resource
    Electronic Resource
    Monosomic additions in beet (Beta vulgaris) carrying extra chromosomes of Beta procumbens (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 577-586 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Beta procumbens ; Alien monosomic additions ; Isozyme markers ; Chromosome identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Eleven isozyme systems were used to identify the extra chromosomes, originating from Beta procumbens, in progenies of 33 monosomic additions in beet (B. vulgaris). Nine groups of monosomic additions could be distinguished, representing the nine different chromosome types of B. procumbens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260912
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  • 6
    Electronic Resource
    Electronic Resource
    Rapid hybridization-based assays for identification by DNA probes of male-sterile and male-fertile cytoplasms of the sugar beet Beta vulgaris L. (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 819-824 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Cytoplasmic male sterility ; mtDNA probes ; Miniassays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Methods are described whereby hybridization of mitochondrial (mt) DNA with different DNA probes can definitely distinguish male-fertile and and male-sterile (cms) cytoplasms of sugar beet Beta vulgaris L. We have developed two types of miniassays. (1) Comparative methods requiring the isolation and restriction of total cellular DNA, hybridization with cloned mtDNA fragments from either fertile or male-sterile cytoplasms, and comparison of the hybridization patterns to the fertile-and sterile-specific patterns of mtDNA of sugar beet for the given mtDNA probe. For these analyses, we routinely used 1 g of plant material to determine the type of cytoplasm. (2) Noncomparative (“plus-minus”) methods requiring neither the isolation of pure DNA nor restriction, electrophoresis, or Southern blotting. Instead, alkaline-SDS plant extracts from as little as 50 mg of plant material were dot-blotted and hybridized with fertile-specific (mitochondrial minicircular DNA) and/or cms-specific probes (consisting of a 2.3-kb mtDNA sequence exclusively occurring in the cms cytoplasm). The assays are simple to perform, give definitive results, are nonde-structive to the plants, and may be used in mass screening of sugar beet populations for hybrid production or in in vitro culture processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224996
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  • 7
    Electronic Resource
    Electronic Resource
    Isozyme analysis of primary trisomies in beet (Beta vulgaris L.). Genetical characterization and techniques for chromosomal assignment of two enzyme-coding loci: leucine aminopeptidase and glutamate oxaloacetate transaminase (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 761-768 
    Details
    ISSN: 1432-2242
    Keywords: Primary trisomies ; Beet ; Beta vulgaris ; Isozyme polymorphism ; Chromosomal assignment ; Distorted segregation ; Dosage shift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Segregating families of beet (Beta vulgaris) were used to verify the monofactorial inheritance of two enzyme-coding loci, leucine aminopeptidase (Lap1) and glutamate oxaloacetate transaminase (Got3). With a series of primary trisomies and using three methods to discriminate between the critical trisomic (the locus is situated on the triplicated chromosome) and the non-critical ones, it was possible to allocate the two loci to beet chromosomes I and II, respectively. For the locus Lap1 distorted segregation ratios were estimated, and the incorporation of three alleles into one plant was attempted. In the case of Got3 the measurement of the allele dosage effect after electrophoresis was chosen as the major strategy. The output of laser densitometric scans were subjected to the non-parametrical Wilcoxon-Mann-Whitney test.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222667
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  • 8
    Electronic Resource
    Electronic Resource
    The relation between cell proliferation, differentiation and ultrastructural development in rat intestinal epithelium (1976)
    Springer
    Cell & tissue research 174 (1976), S. 183-199 
    Details
    ISSN: 1432-0878
    Keywords: Rat small intestine ; Differentiation ; Ultrastructure ; Morphometry ; X-irradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural development of the principal cells in rat small intestine was studied by morphometric analyses in relation to the exact cell position along crypt and villus. From the bottom to the tip of the crypt, a gradual increase occurred in absolute size of the total cell, the cytoplasm, the terminal web and of nearly all cell organelles. Also, the relative size of the cytoplasm, mitochondria, microvilli and endoplasmic reticulum increased during crypt cell differentiation. No sudden changes in ultrastructure were observed in the so-called “critical decision zone”, normally located halfway up the crypt where the proliferative activity ceases. At the crypt-villous junction a 1.4–3 fold increase in cell size, cytoplasm, terminal web and of most organelles was noted. Expansion of the proliferative cell compartment over the total length of the crypt as occurs during recovery after a low X-irradiation dose (72 h after 400 R) does not affect the normal development of cellular ultrastructure. These findings are discussed in relation to biochemical and cell kinetic data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222158
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  • 9
    Electronic Resource
    Electronic Resource
    Neurosecretion in the basommatophoran snail Bulinus truncatus (Gastropoda, Pulmonata) (1977)
    Springer
    Cell & tissue research 176 (1977), S. 57-67 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Alcian blue ; Alcian yellow staining ; Ultrastructure ; Bulinus truncatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neurosecretory system of the freshwater snail Bulinus truncatus was investigated. With the Alcian blue-Alcian yellow (AB/AY) staining method at least 10 different types of neurosecretory cells (NSC) were distinguished in the ganglia of the central nervous system. The differences in staining properties of the NSC — with AB/AY the cells take on different shades of green and yellow — are borne out at the ultrastructural level: the NSC types contain different types of neurosecretory elementary granules. The neurosecretory system of B. truncatus is compared to that of Lymnaea stagnalis, the species which has received the most attention among the pulmonates. It appears from the comparison that the systems of both species show many similarities, although some differences are also apparent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220344
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  • 10
    Electronic Resource
    Electronic Resource
    The pit cell: Description of a new type of cell occurring in rat liver sinusoids and peripheral blood (1976)
    Springer
    Cell & tissue research 173 (1976), S. 423-435 
    Details
    ISSN: 1432-0878
    Keywords: Liver ; Rat ; Sinusoid ; Pit cell ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pit cells — a new type of cell first described here and so named because they contain highly characteristic granules — are situated in the wall of rat liver sinusoids, and have hyaloplasmic pseudopodia intermingling with the microvilli of the parenchymal cells. The characteristic granules are mainly situated at one side of the nucleus, the other side showing organelle-free hyaloplasm. Pit cells are also found in portal tracts and in granuloma-like cellular aggregates. They also occur in rat peripheral blood, although there are morphological differences between cells in these two sites. Pit cells can be regarded as regular inhabitants of the sinusoidal wall, and therefore belong to the series of sinusoidal cells, i.e., the endothelial (Wisse, 1972), Kupffer (Widmann et al., 1972; Wisse and Daems, 1970; Wisse, 1974a,b), and fat-storing cells (Ito, 1973). Pit cells do not phagocytose and do not react to a great number of experimental conditions, to which endothelial and Kupffer cells do react (Wisse, 1972, 1974b). Mitosis has been observed in a pit cell. The function of pit cells remains obscure, but an endocrine function is suggested by the morphology of their highly characteristic granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224305
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