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  • Candida albicans  (5)
  • 65H10  (4)
  • Springer  (9)
  • American Chemical Society
  • MDPI Publishing
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  • 1
    Electronic Resource
    Electronic Resource
    Changes in the cell wall glycoprotein composition of Candida albicans associated to the inhibition of germ tube formation by EDTA (1994)
    Springer
    Archives of microbiology 161 (1994), S. 489-494 
    Details
    ISSN: 1432-072X
    Keywords: Candida albicans ; Dimorphism ; Cell wall ; Mannoprotein ; EDTA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hyphal development in Candida albicans was blocked by EDTA. This effect was not due to a general growth inhibition since the chelator did not affect protein and DNA synthesis. Recovery of mycelial growth was observed when EDTA-grown cells were incubated at 37°C in EDTA-free medium. High-molecular-weight mannoproteins (HMWM) that are mycelium-specific wall components, and particularly a 260-kDa species (HMWM-260), were absent in the wall of cells grown under germination conditions in the presence of EDTA. Synthesis of the HMWM-260 species was not inhibited but its incorporation (secretion) into the wall structure seemed to be blocked in EDTA-treated cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307769
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  • 2
    Electronic Resource
    Electronic Resource
    A Quasi-Newton method with modification of one column per iteration (1984)
    Springer
    Computing 33 (1984), S. 353-362 
    Details
    ISSN: 1436-5057
    Keywords: 65H10 ; Nonlinear systems ; Quasi-Newton methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Wir stellen ein neues Quasi-Newton-Verfahren vor zur Lösung von nichtlinearen simultanen Gleichungen. Bei jeder Iteration wird lediglich eine Spalte vonB k verändert, umB k+1 zu erhalten. Dies erlaubt, wohlbekannte Techniken der Linearen Programmierung zur Faktorisierung vonB k zu benützen. Wir beweisen einen Satz über die lokale Konvergenz für die Methode. Der neue Algorithmus wird mit anderen bezüglich seiner numerischen Eigenschaften verglichen.
    Notes: Abstract In this paper we introduce a new Quasi-Newton method for solving nonlinear simultaneous equations. At each iteration only one column ofB k is changed to obtainB k+1 . This permits to use the well-known techniques of Linear Programming for modifying the factorization ofB k . We present a local convergence theorem for a restarted version of the method. The new algorithm is compared numerically with some other methods which were introduced for solving the same kind of problems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02242278
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  • 3
    Electronic Resource
    Electronic Resource
    Relationship between thigmotropism and Candida biofilm formation in vitro (1998)
    Springer
    Mycopathologia 144 (1998), S. 125-129 
    Details
    ISSN: 1573-0832
    Keywords: biofilm ; Candida albicans ; Candida tropicalis ; saliva ; serum ; thigmotropism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The biofilm formation of the oral fungal pathogen Candida on denture acrylic strips coated with saliva or serum was examined in relation to the ability to induce hyphae by thigmotropic reaction, using C. albicans (4 isolates), C. glabrata (3 isolates) and C. tropicalis (3 isolates). Both the degree of biofilm formation and the amount of hyphae exhibiting thigmotropism varied depending upon both the species and strains of Candida. Although there was no significant correlation between the amount of hyphae induced by thigmotropic reaction of fungal isolates and biofilm formation on uncoated control specimens (r = 0.577; p 〈 0.05), the ability of hyphae induced by thigmotropic reaction significantly correlated with the amount of both saliva- and serum-admixed biofilms (r = 0.734; p 〈 0.05 and r = 0.793; p 〈 0.01, respectively). Taken together our in vitro data suggested that the hyphal induction by thigmotropic reaction is of importance in candidal biofilm formation on saliva- or serum-coated acrylic surfaces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007073930933
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  • 4
    Electronic Resource
    Electronic Resource
    Common and form-specific cell wall antigens of Candida albicans as released by chemical and enzymatic treatments (1996)
    Springer
    Mycopathologia 134 (1996), S. 13-20 
    Details
    ISSN: 1573-0832
    Keywords: Candida albicans ; cell wall ; protein and mannoprotein antigens ; Zymolyase ; β-mercaptoethanol ; polyclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In order to investigate the antigenic properties of the proteins and mannoproteins present in the cell surface of Candida albicans, and to identify individual antigenic moieties and their distribution, a number of polyclonal antisera were obtained by immunizing rabbits with chemical and enzymatic cell wall extracts obtained from intact cells from both growth forms (yeast and mycelium) of the fungus. Prior to injection, wall moieties present in the extracts were subjected to different treatments and/or purification procedures such as adsorption onto polystyrenelatex microbeads or electrophoretic separation. When used as probes in indirect immunofluorescence assays, the different antisera gave rise to different fluorescence patterns varying in intensity and topological localization of the reactivity in C. albicans cells. When the different antisera were used as probes in Western blots of wall proteinaceous materials solubilized from both blastospores and germ tubes, differences in reactivity and specificity were readily discernible, allowing to identify a number of common and form-specific cell wall components. Of special interest was the fact that one of the antisera raised, after adsorption onto heat-killed blastospores, specifically recognized medium to low molecular weight moieties present only in the cell wall extracts from germ tubes. When this antiserum was used as probe in immunofluorescence assays, reactivity was confined to the hyphal extensions. Together, these observations seem to indicate that the different antibody preparations described in this report could represent important tools in the study of different aspects of the cell wall biology in C. albicans, including the identification and study of the distribution of common and form-specific cell wall-bound antigens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437047
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  • 5
    Electronic Resource
    Electronic Resource
    Solution of nonlinear systems of equations by an optimal projection method (1986)
    Springer
    Computing 37 (1986), S. 59-70 
    Details
    ISSN: 1436-5057
    Keywords: 65H10 ; Nonlinear equations ; projection methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Wir betrachten eine Block-Version des Nichtlinearen Projektions-Verfahrens mit einer optimalen Kontrolle. Dieses Verfahren wird zur Lösung von Gleichungssystemen verwendet, bei denen die Gleichungsanzahl kleiner oder gleich der Anzahl der Unbekannten ist. Ein lokaler Konvergenzsatz wird bewiesen. Ein numerischer Vergleich mit dem zyklischen Nichtlinearen Projektions-Verfahren wird angestellt.
    Notes: Abstract We consider a block version of the Nonlinear Projection Method under an optimal control. This method is applied to the solution of systems of equations where the number of equation is less than or equal to the number of unknowns. A local convergence theorem is proved. We present a numerical comparison with the cyclic Nonlinear Projection Method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02252734
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  • 6
    Electronic Resource
    Electronic Resource
    Quasi-Newton methods with factorization scaling for solving sparse nonlinear systems of equations (1987)
    Springer
    Computing 38 (1987), S. 133-141 
    Details
    ISSN: 1436-5057
    Keywords: 65H10 ; Nonlinear systems ; Quasi-Newton methods ; sparse matrices ; factorization of matrices
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Wir stellen in dieser Arbeit ein Verfahren vom Quasi-Newton-Typ für große, dünnbesetzte nichtlineare Gleichungssysteme vor, das die QR-Faktorisierung der näherungsweisen Jacobi-Matrix benutzt. Das Verfahren gehört zu einer allgemeinen Klasse von Algorithmen, für die wir die lokale Konvergenz beweisen. Einige numerische Experimente deuten auf die Verläßlichkeit des neuen Algorithmus hin.
    Notes: Abstract In this paper we present a Quasi-Newton type method, which applies to large and sparse nonlinear systems of equations, and uses the Q-R factorization of the approximate Jacobians. This method belongs to a more general class of algorithms for which we prove a local convergence theorem. Some numerical experiments seem to confirm that the new algorithm is reliable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02240178
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  • 7
    Electronic Resource
    Electronic Resource
    Preliminary characterization of the material released to the culture medium byCandida albicans yeast and mycelial cells (1995)
    Springer
    Antonie van Leeuwenhoek 68 (1995), S. 195-201 
    Details
    ISSN: 1572-9699
    Keywords: Candida albicans ; cell wall ; culture filtrates ; mannoproteins ; proteins ; surface antigens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Culture filtrate concentrates were obtained fromCandida albicans yeast and mycelial cells grown in the presence of14C-protein hydrolysate for radioactive labeling of cellular polypeptides. Both growth forms released to the medium minor but significant amounts of proteinaceous materials. The analysis of culture filtrate concentrates by means of SDS-polyacrylamide gel electrophoresis and fluorography revealed a similar and complex electrophoretic pattern, though some qualitative and quantitative differences between samples obtained from yeast and mycelial cells were observed. Materials released, mostly composed of mannoproteins as shown by their affinity towards concanavalin A, presented (i) cross-reactivity (by Western immunoblotting and ELISA) against polyclonal antisera to genuine cell wall components (among them the 58-kilodalton fibrinogen-binding mannoprotein) and (ii) high affinity for polystyrene-latex microbeads. Results presented suggest a possible common identity for the molecules shed to the medium and the cell wall protein and mannoprotein constituents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00871815
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  • 8
    Electronic Resource
    Electronic Resource
    A comparative study on cell wall antigens and cell surface hydrophobicity in clinical isolates ofCandida albicans (1994)
    Springer
    Mycopathologia 127 (1994), S. 1-13 
    Details
    ISSN: 1573-0832
    Keywords: Candida albicans ; Cell surface hydrophobicity ; Cell wall ; Clinical strains ; Protein and mannoprotein antigens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Characterization of common cell surface-bound antigens inCandida albicans strains, particularly those expressed in the walls of mycelial cells might be useful in the diagnosis of systemic candidiasis. Hence, antigenic similarities among wall proteins and mannoproteins fromC. albicans clinical serotype A and B isolates, were studied using polyclonal (mPAbs) and monoclonal (MAb 4C12) antibodies raised against wall antigens from the mycelial form of a commonC. albicans serotype A laboratory strain (ATCC 26555). Zymolyase digestion of walls isolated from cells of the different strains studied grown at 37°C (germination conditions), released, in all cases, numerous protein and mannoprotein components larger than 100 kDa, along with a 33–34 kDa species. The pattern of major antigens exhibiting reactivity towards the mPAbs preparation was basically similar for all the serotype A and B isolates, though minor strain-specific bands were also observed. The immunodeterminant recognized by MAb 4C12 was found to be absent or present in very low amounts inC. albicans isolates other than the ATCC 26555 strain, yet high molecular weight species similar in size (e.g., 260 kDa) to the wall antigen against which MAb 4C12 was raised, were observed, particularly in wall digests from serotype A strains. Cell surface hydrophobicity, an apparently important virulence factor inC. albicans, of the cell population of each serotype B strain was lower than that of the corresponding serotype A counterparts, which is possibly due to the fact that the former strains exhibited a reduced ability to form mycelial filaments under the experimental conditions used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01104005
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  • 9
    Electronic Resource
    Electronic Resource
    Parallel implementations of Broyden's method (1992)
    Springer
    Computing 47 (1992), S. 361-366 
    Details
    ISSN: 1436-5057
    Keywords: 65H10 ; Nonlinear systems ; Broyden's method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Das Verfahren von Broyden liefert einen der effizientesten Algorithmen zur Lösung nichtlinearer Gleichungssysteme. Bei sehr vielen Gleichungen und Unbekannten werden häufig Implementierungen ohne Zwischenspeicherung verwendet (Gomes-Ruggiero, Martinez und Moretti, SIAM J. SCISC 91, erscheint in Kürze). Wir zeigen, daß die Rechnungen so organisiert werden können, daß man ihren Parallelismus ausnützen kann.
    Notes: Abstract Broyden's method is one of the most effective algorithms for solving nonlinear systems of equations. When the number of equations and unknowns is very large, memoryless implementations of this method are frequently used. We analyze one of this implementations (Gomes-Ruggiero, Martínez and Moretti, SIAM J. Sci. Stat. Comput. 1991, to appear) and we show that calculations may be organized in such a way that parallelism can be exploited.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02320203
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