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  • Engineering  (1)
  • HIS3 heterologous auxotrophic marker  (1)
  • Wiley-Blackwell  (2)
  • American Chemical Society
  • MDPI
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  • Wiley-Blackwell  (2)
  • American Chemical Society
  • MDPI
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  • 1
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 7 (1973), S. 479-490 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: FORTRAN subprograms for the solution of systems of linear algebraic equations are evaluated and compared on the basis of execution speed and accuracy. A symmetric, positive definite, banded test matrix is used in each case. The procedures considered are direct solution, iteration and matrix inversion. Both in-core schemes and those requiring the use of auxiliary data storage devices are included. Some of the techniques used require the full coefficient matrix, whereas others account for symmetry, banding, or sparseness of the system.Matrix inversion is found to be an inefficient technique, even if multiple right-hand side constant vectors are to be solved. In such cases, either Gauss elimination with multiple constant vectors treated simultaneously, or decomposition with the retention of the upper and lower triangular matrices, is recommended. Double precision arithmetic is suggested as a means of reducing round-off errors, and should always be employed when permitted by the computer's core capacity.On the basis of the results obtained with the single test system of equations, specific subprograms are recommended for each category of problem.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0749-503X
    Keywords: PCR-targeting ; pFA plasmids ; HIS3 heterologous auxotrophic marker ; geneticin ; green fluorescence protein ; kanMX ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have fused the open reading frames of his3-complementing genes from Saccharomyces kluyveri and Schizosaccharomyces pombe to the strong TEF gene promotor of the filamentous fungus Ashbya gossypii. Both chimeric modules and the cognate S. kluyveri HIS3 gene were tested in transformations of his3 S. cerevisiae strains using PCR fragments flanked by 40 bp target guide sequences. The 1·4 kb chimeric Sz. pombe module (HIS3MX6) performed best. With less than 5% incorrectly targeted transformants, it functions as reliably as the widely used geniticin resistance marker kanMX. The rare false-positive His+ transformants seem to be due to non-homologous recombination rather than to gene conversion of the mutated endogenous his3 allele. We also cloned the green fluorescent protein gene from Aequorea victoria into our pFA-plasmids with HIS3MX6 and kanMX markers. The 0·9 kb GFP reporters consist of wild-type GFP or GFP-S65T coding sequences, lacking the ATG, fused to the S. cerevisiae ADH1 terminator. PCR-synthesized 2·4 kb-long double modules flanked by 40-45 bp-long guide sequences were successfully targeted to the carboxy-terminus of a number of S. cerevisiae genes. We could estimate that only about 10% of the transformants carried inactivating mutations in the GFP reporter. © 1997 by John Wiley & Sons, Ltd.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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