ISSN:
1573-5028
Keywords:
DNA repair
;
Glycine max
;
plastome
;
quantitative PCR
;
suspension cells
;
UV damage
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In order to better understand the biochemical mechanisms of DNA metabolism in chloroplasts, repair of UV induced plastome damage in vivo was determined by exposure of soybean suspension cells to UV light and subsequent quantitation of the damage remaining in nuclear and chloroplast encoded genes with time by quantitative polymerase chain reaction (QPCR). The kinetics of damage rapir in the nuclear rbcS gene suggest that photoreactivation and dark mechanisms are active, while for the plastome encoded psbA gene only a light-dependent repair process was detected which is considerably slower than would be expected for photolyase-mediated photoreactivation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00020467
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