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  • 05.04. Instrumentation and techniques of general interest  (1)
  • Budesonide  (1)
  • Springer  (2)
  • American Chemical Society
  • Cell Press
  • Institute of Physics
  • 1
    ISSN: 1432-1041
    Keywords: Budesonide ; liver ; man ; sulphotransferase ; testosterone ; drug metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Budesonide, a corticosteroid used in the treatment of asthma and allergic reactions, is almost entirely cleared by metabolism in man. We describe the sulphation of budesonide in human liver and lung and provide evidences that the sulphation of budesonide is catalysed by testosterone sulphotransferase. A rapid and reproducible radiometric assay for budesonide sulphotransferase is described. Liver specimens were obtained from 35 men and 65 women and lung specimens from 2 women and 17 men. The average hepatic budesonide sulphation rate was significantly higher in men (41.1 pmol·min−1·ml−1) than women (28.2 pmol·min−1·mg−1). In the lung, the mean budesonide sulphation rate was 5.0 pmol·min−1·mg−1. Testosterone strongly inhibited the hepatic sulphation of budesonide, whereas p-nitrophenol and dopamine were poor inhibitors; the IC50 was 7.0 uM (testosterone), 320 uM (p-nitrophenol) and 510 uM (dopamine). The hepatic rates of testosterone, p-nitrophenol and dopamine sulphation were measured in the same samples assayed for budesonide sulphotransferase. There was a correlation between the hepatic rates of budesonide and testosterone sulphation (P〈0.001; r=0.810). The activity of testosterone sulphotransferase was significantly greater in men than women (22.0 vs. 17.2 pmol·min−1·mg−1), wheres those of dopamine and p-nitrophenol sulphotransferase were not sex dependent. The hepatic activity of budesonide sulphotransferase parallels that of testosterone suggesting that sulphation is an important reaction in the metabolism of budesonide.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2020-11-12
    Description: Public concern about anthropogenic seismic- ity in Italy first arose in the aftermath of the deadly M ≈ 6 earthquakes that hit the Emilia-Romagna region (northern Italy) in May 2012. As these events occurred in a (tectonically active) region of oil and gas production and storage, the question was raised, whether stress perturbations due to underground industrial activities could have induced or triggered the shocks. Following expert recommendations, in 2014, the Italian Oil & Gas Safety Authority (DGS-UNMIG, Ministry of Economic Development) published guidelines (ILG - Indirizzi e linee guida per il monitoraggio della sismicità, delle deformazioni del suolo e delle pressioni di poro nell’ambito delle attività antropiche), describing regula- tions regarding hydrocarbon extraction, waste-water in- jection and gas storage that could also be adapted to other technologies, such as dams, geothermal systems, CO2 storage, and mining. The ILG describe the frame- work for the different actors involved in monitoring activities, their relationship and responsibilities, the procedure to be followed in case of variations of mon- itored parameters, the need for in-depth scientific anal- yses, the definition of different alert levels, their mean- ing and the parameters to be used to activate such alerts. Four alert levels are defined, the transition among which follows a decision to be taken jointly by relevant au- thorities and industrial operator on the basis of evalua- tion of several monitored parameters (micro-seismicity, ground deformation, pore pressure) carried on by a scientific-technical agency. Only in the case of liquid reinjection, the alert levels are automatically activated on the basis of exceedance of thresholds for earthquake magnitude and ground shaking – in what is generally known as a Traffic Light System (TLS). Istituto Nazionale di Geofisica e Vulcanologia has been charged by the Italian oil and gas safety authority (DGS- UNMIG) to apply the ILG in three test cases (two oil extraction and one gas storage plants). The ILG indeed represent a very important and positive innovation, as they constitute official guidelines to coherently regulate monitoring activity on a national scale. While pilot studies are still mostly under way, we may point out merits of the whole framework, and a few possible critical issues, requiring special care in the implementa- tion. Attention areas of adjacent reservoirs, possibly licenced to different operators, may overlap, hence mak- ing the point for joint monitoring, also in view of the possible interaction between stress changes related to the different reservoirs. The prescribed initial blank- level monitoring stage, aimed at assessing background seismicity, may lose significance in case of nearby ac- tive production. Magnitude – a critical parameter used to define a possible step-up in activation levels – has inherent uncertainty and can be evaluated using differ- ent scales. A final comment considers the fact that relevance of TLS, most frequently used in hydraulic fracturing operations, may not be high in case of trig- gered tectonic events.
    Description: Published
    Description: 1015–1028
    Description: 1IT. Reti di monitoraggio e sorveglianza
    Description: JCR Journal
    Keywords: Anthropogenic seismicity ; Alert system ; Monitoring guidelines ; 04.06. Seismology ; 05.04. Instrumentation and techniques of general interest
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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