ISSN:
1432-2048
Keywords:
Cell culture (cytokinin content)
;
Cytokinin isolation and quantitation
;
Pimpinella
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Using three different techniques, isopentenyladenosine was identified and quantitated in an anise cell line (Pimpinella anisum L.), growing on a medium without cytokinin. A method to quantitate cytokinins was developed which utilizes extraction of cytokinins in the presence of a deuterated reference compound and subsequent quantitation of the cytokinins by single-ion monitoring on a gas chromatography-mass spectrometry system. The results were compared with those obtained by radioimmunoassay as well as a bioassay. Quantitations by gas chromatography-mass spectrometry and radioimmunoassay correlated well, whereas the estimates by the bioassay gave considerably lower values.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00397527
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